Fig. 2 Flow cytometry gating strategy for immune cell types and OVA-AF647
+ cells. (a) Live immune cells were
gated according to their forward scatter (FSC) and side scatter (SSC) properties, negative LIVE/DEAD™ cell
stain signal and high CD45 expression. Immune cell types were identified according to cell surface expression
of specific markers. (b) Representative dot plots showing the gating of OVA-AF647
+ populations in macrophages from the negative and antigen alone groups. Representative bar graphs showing total (c) and
OVA-AF647
+ (d) cell numbers per immune cell type