Acknowledgments
This work was supported by the Nanotechnology Research Center
and the Human Health Therapeutics Research Center at the
National Research Council of Canada.
References
1. Dubochet J et al (1988) Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 21
(2):129–228
2. Bartesaghi A et al (2018) Atomic resolution
cryo-EM structure of β-galactosidase. Structure 26:848
3. Merk A et al (2016) Breaking cryo-EM resolution barriers to facilitate drug discovery. Cell
165:1698–1707
4. Kuntsche J et al (2011) Cryogenic transmission electron microscopy (cryo-TEM) for
studying the morphology of colloidal drug
delivery systems. Int J Pharm 417:120–137
5. Venkataraman S et al (2011) The effects of
polymeric nanostructure shape on drug delivery. Adv Drug Deliv Rev 63:1228–1246
6. Paul AL et al (2012) Immunoelectron microscopy: a reliable tool for the analysis of cellular
processes. In: Applications of immunocytochemistry. InTech, Rijeka, Croatia, pp 65–96
7. Jia Y et al (2019) A comparison of the immune
responses induced by antigens in three
different archaeosome-based vaccine formulations. Int J Pharm 561:187–196
8. Whitfield DM et al (2016) Sulfated-glycolipids
as adjuvants for vaccines. National Research
Council of Canada. Patent No. WO/2016/
004512
9. Aebi U et al (1987) A glow discharge unit to
render electron microscope grids and other
surfaces hydrophilic. J Electron Microsc Tech
7:29–33
10. Egerton RF (2013) Control of radiation damage
in
the
TEM.
Ultramicroscopy
127:100–108
11. Glaeser RM (1971) Limitations to significant
information in biological electron microscopy
as a result of radiation damage. J Ultrastruct
Res 36:466–482
12. Lawrence MC (1992) Least-squares methods
of alignment using markers. In: Frank J
(ed)
Electron
tomography.
Plenum,
New York, pp 197–204
Cryo-TEM for Vaccine Delivery Carrier Evaluation
511
This work was supported by the Nanotechnology Research Center
and the Human Health Therapeutics Research Center at the
National Research Council of Canada.
References
1. Dubochet J et al (1988) Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 21
(2):129–228
2. Bartesaghi A et al (2018) Atomic resolution
cryo-EM structure of β-galactosidase. Structure 26:848
3. Merk A et al (2016) Breaking cryo-EM resolution barriers to facilitate drug discovery. Cell
165:1698–1707
4. Kuntsche J et al (2011) Cryogenic transmission electron microscopy (cryo-TEM) for
studying the morphology of colloidal drug
delivery systems. Int J Pharm 417:120–137
5. Venkataraman S et al (2011) The effects of
polymeric nanostructure shape on drug delivery. Adv Drug Deliv Rev 63:1228–1246
6. Paul AL et al (2012) Immunoelectron microscopy: a reliable tool for the analysis of cellular
processes. In: Applications of immunocytochemistry. InTech, Rijeka, Croatia, pp 65–96
7. Jia Y et al (2019) A comparison of the immune
responses induced by antigens in three
different archaeosome-based vaccine formulations. Int J Pharm 561:187–196
8. Whitfield DM et al (2016) Sulfated-glycolipids
as adjuvants for vaccines. National Research
Council of Canada. Patent No. WO/2016/
004512
9. Aebi U et al (1987) A glow discharge unit to
render electron microscope grids and other
surfaces hydrophilic. J Electron Microsc Tech
7:29–33
10. Egerton RF (2013) Control of radiation damage
in
the
TEM.
Ultramicroscopy
127:100–108
11. Glaeser RM (1971) Limitations to significant
information in biological electron microscopy
as a result of radiation damage. J Ultrastruct
Res 36:466–482
12. Lawrence MC (1992) Least-squares methods
of alignment using markers. In: Frank J
(ed)
Electron
tomography.
Plenum,
New York, pp 197–204
Cryo-TEM for Vaccine Delivery Carrier Evaluation
511