3.1.1 Preparation
of In-House Vaccine: In Situ
Adsorption of OVA onto
Alhydrogel
®
Alhydrogel
® is a commercially available 2% (w/v) aluminum
hydroxide wet gel. It is recommended to identify the optimal
binding efficiency between the antigen of interest and the aluminum (oxy)hydroxide particles in Alhydrogel
® to prepare a
vaccine [6].
1. Vortex Alhydrogel
® suspension briefly to break up the large
coagulates.
2. Use 5 N NaOH or 5 N acetic acid to adjust the pH to a desired
value, if needed.
Rotating
Filled with liquid nitrogen
Stainless steel blade
Lyophilization
Liquid nitrogen
a
b
Lyophilization
Fig. 2 Schematic of thin-film freezing of vaccine in liquid using a TFF apparatus
(a) or in a single vial (b) followed by lyophilization
Thin-Film Freeze-Drying Is a Viable Method to Convert Vaccines Containing. . .
493
of In-House Vaccine: In Situ
Adsorption of OVA onto
Alhydrogel
®
Alhydrogel
® is a commercially available 2% (w/v) aluminum
hydroxide wet gel. It is recommended to identify the optimal
binding efficiency between the antigen of interest and the aluminum (oxy)hydroxide particles in Alhydrogel
® to prepare a
vaccine [6].
1. Vortex Alhydrogel
® suspension briefly to break up the large
coagulates.
2. Use 5 N NaOH or 5 N acetic acid to adjust the pH to a desired
value, if needed.
Rotating
Filled with liquid nitrogen
Stainless steel blade
Lyophilization
Liquid nitrogen
a
b
Lyophilization
Fig. 2 Schematic of thin-film freezing of vaccine in liquid using a TFF apparatus
(a) or in a single vial (b) followed by lyophilization
Thin-Film Freeze-Drying Is a Viable Method to Convert Vaccines Containing. . .
493