consequences: beyond the earache. Lancet
Infect Dis 10(3):195–203. https://doi.org/
10.1016/S1473-3099(10)70012-8
3. Walker CLF, Rudan I, Liu L, Nair H,
Theodoratou E, Bhutta ZA, O’Brien KL,
Campbell H, Black RE (2013) Global burden
of childhood pneumonia and diarrhoea. Lancet
381(9875):1405–1416. https://doi.org/10.
1016/S0140-6736(13)60222-6
4. Adetifa IMO, Adamu AL, Karani A,
Waithaka M, Odeyemi KA, Okoromah CAN,
Bello MM, Abubakar IS, Inem V, Scott JAG
(2018) Nasopharyngeal pneumococcal carriage in Nigeria: a two-site, population-based
survey. Sci Rep 8(1):3509. https://doi.org/
10.1038/s41598-018-21837-5
5. Jones CH, Zhang G, Nayerhoda R,
Beitelshees M, Hill A, Rostami P, Li Y, Davidson BA, Knight P 3rd, Pfeifer BA (2017) Comprehensive vaccine design for commensal
disease progression. Sci Adv 3(10):e1701797.
https://doi.org/10.1126/sciadv.1701797
6. World Health Organization (2017, February
27) WHO publishes list of bacteria for which
new antibiotics are urgently needed. https://
2017-who-publishes-list-of-bacteria-forwhich-new-antibiotics-are-urgently-needed
7. Papadatou I, Spoulou V (2016) Pneumococcal
vaccination in high-risk individuals: are we
doing it right? Clin Vaccine Immunol 23
(5):388–395. https://doi.org/10.1128/CVI.
00721-15
8. Saokaew S, Rayanakorn A, Wu DB, Chaiyakunapruk N (2016) Cost effectiveness of pneumococcal vaccination in children in low- and
middle-income countries: a systematic review.
Pharmacoeconomics
34(12):1211–1225.
https://doi.org/10.1007/s40273-016-04393
9. Hill AB, Beitelshees M, Nayerhoda R, Pfeifer
BA, Jones CH (2018) Engineering a nextgeneration glycoconjugate-like Streptococcus
pneumoniae vaccine. ACS Infect Dis 4:1553.
https://doi.org/10.1021/acsinfecdis.
8b00100
10. Li Y, Hill A, Beitelshees M, Shao S, Lovell JF,
Davidson BA, Knight PR, Hakansson AP, Pfeifer BA, Jones CH (2016) Directed vaccination
against pneumococcal disease. Proc Natl Acad
Sci U S A 113(25):6898–6903
11. Foteini P, Pippa N, Naziris N, Demetzos C
(2019) Physicochemical study of the proteinliposome interactions: influence of liposome
composition and concentration on protein
binding. J Liposome Res 29:313–321.
https://doi.org/10.1080/08982104.2018.
1468774
12. Nele V, Holme MN, Kauscher U, Thomas MR,
Doutch JJ, Stevens MM (2019) Effect of formulation method, lipid composition, and
PEGylation on vesicle lamellarity: a smallangle neutron scattering study. Langmuir 35
(18):6064–6074. https://doi.org/10.1021/
acs.langmuir.8b04256
13. Kim JS, Laskowich ER, Arumugham RG, Kaiser RE, MacMichael GJ (2005) Determination
of saccharide content in pneumococcal polysaccharides and conjugate vaccines by GC-MSD.
Anal Biochem 347(2):262–274. https://doi.
org/10.1016/j.ab.2005.09.022
14. Masuko T, Minami A, Iwasaki N, Majima T,
Nishimura S, Lee YC (2005) Carbohydrate
analysis by a phenol-sulfuric acid method in
microplate format. Anal Biochem 339
(1):69–72.
https://doi.org/10.1016/j.ab.
2004.12.001
Liposomal Dual Antigen Delivery
487
Infect Dis 10(3):195–203. https://doi.org/
10.1016/S1473-3099(10)70012-8
3. Walker CLF, Rudan I, Liu L, Nair H,
Theodoratou E, Bhutta ZA, O’Brien KL,
Campbell H, Black RE (2013) Global burden
of childhood pneumonia and diarrhoea. Lancet
381(9875):1405–1416. https://doi.org/10.
1016/S0140-6736(13)60222-6
4. Adetifa IMO, Adamu AL, Karani A,
Waithaka M, Odeyemi KA, Okoromah CAN,
Bello MM, Abubakar IS, Inem V, Scott JAG
(2018) Nasopharyngeal pneumococcal carriage in Nigeria: a two-site, population-based
survey. Sci Rep 8(1):3509. https://doi.org/
10.1038/s41598-018-21837-5
5. Jones CH, Zhang G, Nayerhoda R,
Beitelshees M, Hill A, Rostami P, Li Y, Davidson BA, Knight P 3rd, Pfeifer BA (2017) Comprehensive vaccine design for commensal
disease progression. Sci Adv 3(10):e1701797.
https://doi.org/10.1126/sciadv.1701797
6. World Health Organization (2017, February
27) WHO publishes list of bacteria for which
new antibiotics are urgently needed. https://
2017-who-publishes-list-of-bacteria-forwhich-new-antibiotics-are-urgently-needed
7. Papadatou I, Spoulou V (2016) Pneumococcal
vaccination in high-risk individuals: are we
doing it right? Clin Vaccine Immunol 23
(5):388–395. https://doi.org/10.1128/CVI.
00721-15
8. Saokaew S, Rayanakorn A, Wu DB, Chaiyakunapruk N (2016) Cost effectiveness of pneumococcal vaccination in children in low- and
middle-income countries: a systematic review.
Pharmacoeconomics
34(12):1211–1225.
https://doi.org/10.1007/s40273-016-04393
9. Hill AB, Beitelshees M, Nayerhoda R, Pfeifer
BA, Jones CH (2018) Engineering a nextgeneration glycoconjugate-like Streptococcus
pneumoniae vaccine. ACS Infect Dis 4:1553.
https://doi.org/10.1021/acsinfecdis.
8b00100
10. Li Y, Hill A, Beitelshees M, Shao S, Lovell JF,
Davidson BA, Knight PR, Hakansson AP, Pfeifer BA, Jones CH (2016) Directed vaccination
against pneumococcal disease. Proc Natl Acad
Sci U S A 113(25):6898–6903
11. Foteini P, Pippa N, Naziris N, Demetzos C
(2019) Physicochemical study of the proteinliposome interactions: influence of liposome
composition and concentration on protein
binding. J Liposome Res 29:313–321.
https://doi.org/10.1080/08982104.2018.
1468774
12. Nele V, Holme MN, Kauscher U, Thomas MR,
Doutch JJ, Stevens MM (2019) Effect of formulation method, lipid composition, and
PEGylation on vesicle lamellarity: a smallangle neutron scattering study. Langmuir 35
(18):6064–6074. https://doi.org/10.1021/
acs.langmuir.8b04256
13. Kim JS, Laskowich ER, Arumugham RG, Kaiser RE, MacMichael GJ (2005) Determination
of saccharide content in pneumococcal polysaccharides and conjugate vaccines by GC-MSD.
Anal Biochem 347(2):262–274. https://doi.
org/10.1016/j.ab.2005.09.022
14. Masuko T, Minami A, Iwasaki N, Majima T,
Nishimura S, Lee YC (2005) Carbohydrate
analysis by a phenol-sulfuric acid method in
microplate format. Anal Biochem 339
(1):69–72.
https://doi.org/10.1016/j.ab.
2004.12.001
Liposomal Dual Antigen Delivery
487