3. Once 14 days have passed for mice challenged with the pneumonia challenge model or 21 days have passed for the sepsis
challenge model, mice should be considered to have demonstrated complete survival.
4 Notes
1. It is more time efficient if preparation of the plasmid backbone
is conducted in parallel with preparation of the insert. Ideally,
the E. coli culture used to prepare the backbone should be
inoculated the night before the antigen is PCR amplified.
Fig. 3 Murine immunization and disease challenge timeline
Fig. 2 Preparation of S. pneumoniae for murine challenge models of vaccination effectiveness
Hybrid Biological-Biomaterial Vector
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