4. Tuck the tail between the third and fourth finger of the nondominant hand [15].
3.10 Sublingual
Administration
Animal sedation is necessary for this procedure [16].
1. Sedate the animal.
2. To promote sedation of the animal, use a mixture of xylazine
hydrochloride (10 mg/kg of body weight) and ketamine
(100 mg/kg of body weight), or any other anesthetic authorized by the ethic committee.
3. With the animal restrained, inject the anesthetic mixture intraperitonially (volume calculated according to animal’s weight)
in the animal’s right low quadrant of the abdomen (Fig. 4) (see
Note 12).
Fig. 2 One-hand mice restraint. Pick the mouse by the base of the tail allocating it in a rough surface (a), hold
the tail with the index finger and thumb while tucking the base of the tail between third and fourth finger (b),
press the mouse gently against the surface and, with the thumb and index finger, hold the skin of the back of
its neck (c) and the restraining is completed (d)
Lactic Acid Bacteria for Therapeutic Applications
453
3.10 Sublingual
Administration
Animal sedation is necessary for this procedure [16].
1. Sedate the animal.
2. To promote sedation of the animal, use a mixture of xylazine
hydrochloride (10 mg/kg of body weight) and ketamine
(100 mg/kg of body weight), or any other anesthetic authorized by the ethic committee.
3. With the animal restrained, inject the anesthetic mixture intraperitonially (volume calculated according to animal’s weight)
in the animal’s right low quadrant of the abdomen (Fig. 4) (see
Note 12).
Fig. 2 One-hand mice restraint. Pick the mouse by the base of the tail allocating it in a rough surface (a), hold
the tail with the index finger and thumb while tucking the base of the tail between third and fourth finger (b),
press the mouse gently against the surface and, with the thumb and index finger, hold the skin of the back of
its neck (c) and the restraining is completed (d)
Lactic Acid Bacteria for Therapeutic Applications
453