2 Materials
2.1 Liquid Jet
Injections
1. Spring powered liquid jet injectors.
2. DNA vaccine.
3. Anesthesia—Ketamine and Xylazine.
4. Microfine insulin syringe (29 G).
5. 70% ethanol.
6. 0.9% NaCl.
7. Fine capillary tubes for orbital puncture.
2.2 Particle
Bombardment of Skin
1. Particles of polystyrene, gold of known radii.
2. Spermidine for DNA coating.
3. Commercial gene gun.
2.3 Physical
Methods
1. Microprojection array.
2. 1%, 5%, and 20% solutions of OVA in phosphate buffered
saline.
3. Polyisobutylene adhesive.
4. Isoflurane 3–3.5%.
2.4 Microporation
1. Microporation prototype.
2. Vaccine sample.
2.5 Vaccine Delivery
Using Shock Waves
1. Shock wave device as described.
2. Vaccines to be injected.
3. 70% ethanol for skin surface sterilization
4. Hair removal cream/shaver to clear fur off animals.
2.6 Vaccine Delivery
by Microneedles
1. Microneedle assemble and array.
2. Vaccine sample.
3. 70% ethanol.
3 Methods
3.1 Liquid Jet
Injections
1. Spring powered jet injectors (Isajet™, Prolitec, Aouste/Sye,
France) are used for the intradermal administration of DNA
solutions [36].
2. A low force Isa10 injector is used in mice and the Isa40 injector, a jet-gun designed for human use, is used in monkeys.
3. Solutions to be injected are filled into the 5 ml glass chamber of
the Isa10 or into a syringe which adapts onto the Isa40 injector.
Methods of Needle-Free Injections
441
2.1 Liquid Jet
Injections
1. Spring powered liquid jet injectors.
2. DNA vaccine.
3. Anesthesia—Ketamine and Xylazine.
4. Microfine insulin syringe (29 G).
5. 70% ethanol.
6. 0.9% NaCl.
7. Fine capillary tubes for orbital puncture.
2.2 Particle
Bombardment of Skin
1. Particles of polystyrene, gold of known radii.
2. Spermidine for DNA coating.
3. Commercial gene gun.
2.3 Physical
Methods
1. Microprojection array.
2. 1%, 5%, and 20% solutions of OVA in phosphate buffered
saline.
3. Polyisobutylene adhesive.
4. Isoflurane 3–3.5%.
2.4 Microporation
1. Microporation prototype.
2. Vaccine sample.
2.5 Vaccine Delivery
Using Shock Waves
1. Shock wave device as described.
2. Vaccines to be injected.
3. 70% ethanol for skin surface sterilization
4. Hair removal cream/shaver to clear fur off animals.
2.6 Vaccine Delivery
by Microneedles
1. Microneedle assemble and array.
2. Vaccine sample.
3. 70% ethanol.
3 Methods
3.1 Liquid Jet
Injections
1. Spring powered jet injectors (Isajet™, Prolitec, Aouste/Sye,
France) are used for the intradermal administration of DNA
solutions [36].
2. A low force Isa10 injector is used in mice and the Isa40 injector, a jet-gun designed for human use, is used in monkeys.
3. Solutions to be injected are filled into the 5 ml glass chamber of
the Isa10 or into a syringe which adapts onto the Isa40 injector.
Methods of Needle-Free Injections
441