l
Wait 10 min keeping tubes at RT within a
chemical hood.
l
Vortex each tube for few seconds.
l
Wait 30 min keeping tubes at RT within a
chemical hood.
l
Start the analyses with instrument set as reported
below:
– Spectrophotometer wavelength set at 490 nm and
the lamp turned on at least 1 h prior of readings to
warm up.
Table 2
Dilutions for preparing the neutral sugars calibration curve for HPAEC-PAD analysis
μg/mL (each sugar)
11.25 μg/mL
standards solution (μL)
Water (μL)
0
0
450
0.5
20
430
1.0
40
410
2.5
100
350
5.0
200
250
7.5
300
150
10
400
50
Fig. 4 HPAEC-PAD profile of S. paratyphi A OAg sample compared to the monosaccharide standards
O-Antigen Purification and Conjugation
283
Wait 10 min keeping tubes at RT within a
chemical hood.
l
Vortex each tube for few seconds.
l
Wait 30 min keeping tubes at RT within a
chemical hood.
l
Start the analyses with instrument set as reported
below:
– Spectrophotometer wavelength set at 490 nm and
the lamp turned on at least 1 h prior of readings to
warm up.
Table 2
Dilutions for preparing the neutral sugars calibration curve for HPAEC-PAD analysis
μg/mL (each sugar)
11.25 μg/mL
standards solution (μL)
Water (μL)
0
0
450
0.5
20
430
1.0
40
410
2.5
100
350
5.0
200
250
7.5
300
150
10
400
50
Fig. 4 HPAEC-PAD profile of S. paratyphi A OAg sample compared to the monosaccharide standards
O-Antigen Purification and Conjugation
283