10. Thermomixer (Heidolph Instruments GmbH & Co. KG,
Schwabach, Germany).
11. 0.22
μm
bottle-top
filters
(Corning,
Corning,
New York, USA).
12. Vacuum pump (#181-0067P, VWR International, Radnor,
PA, USA).
13. 500-mL, 250-mL, and 100-mL glass bottles (Carl Roth
GmbH, Karlsruhe, Germany).
14. Ultrasonic bath (Ultrasonic cleaner (VWR International, Radnor, PA, USA).
15. Regenerated cellulose membranes, 1 μm pore size (Whatman
RC60, 50 mm circles, GE Healthcare Life Sciences, Uppsala,
Sweden).
16. 13 mm puncher (hardware store).
17. Rubber hammer (hardware store).
18. Petri dish (between 5 and 10 cm diameter, Sarstedt Nu ¨mbrecht, Germany).
2.2.3 Preparation
of the Sample
1. Clarified virus harvest (from Subheading 2.2.1).
2. PBS supplemented with 16% (w/v) PEG 8000 (from Subheading 2.2.2).
3. 50-mL centrifuge tubes (Sarstedt, Nu ¨mbrecht, Germany).
4. 2-mL reaction tubes (Sarstedt, Nu ¨mbrecht, Germany).
5. Sterile single-use pipette tips (Serological pipettes, Sarstedt,
Nu ¨mbrecht, Germany).
6. Pipette holder/rubber bulb (Pipetus, Hirschmann Laborger€ ate, Eberstadt, Germany).
7. 10-mL single-use syringes (B. Braun, Melsungen, Germany).
8. Single-use cannulas, blunt with Luer lock attachment
(B. Braun, Melsungen, Germany).
9. Luer lock syringe cap male/female (B. Braun, Melsungen,
Germany).
2.2.4 Performing
the SXC
1. 10-mL superloop (GE Healthcare Life Sciences, Uppsala,
Sweden)
2. FPLC system (e.g., A ¨ kta Pure 25; GE Healthcare Life Sciences,
Uppsala, Sweden).
3. Buffers prepared in Subheading 2.2.2.
4. 15-mL centrifuge tubes (Sarstedt, Nu ¨mbrecht, Germany).
5. Inline filter holder device 13 mm (Pall Life Sciences, Port
Washington, NY, USA).
228
Keven Lothert et al.
Schwabach, Germany).
11. 0.22
μm
bottle-top
filters
(Corning,
Corning,
New York, USA).
12. Vacuum pump (#181-0067P, VWR International, Radnor,
PA, USA).
13. 500-mL, 250-mL, and 100-mL glass bottles (Carl Roth
GmbH, Karlsruhe, Germany).
14. Ultrasonic bath (Ultrasonic cleaner (VWR International, Radnor, PA, USA).
15. Regenerated cellulose membranes, 1 μm pore size (Whatman
RC60, 50 mm circles, GE Healthcare Life Sciences, Uppsala,
Sweden).
16. 13 mm puncher (hardware store).
17. Rubber hammer (hardware store).
18. Petri dish (between 5 and 10 cm diameter, Sarstedt Nu ¨mbrecht, Germany).
2.2.3 Preparation
of the Sample
1. Clarified virus harvest (from Subheading 2.2.1).
2. PBS supplemented with 16% (w/v) PEG 8000 (from Subheading 2.2.2).
3. 50-mL centrifuge tubes (Sarstedt, Nu ¨mbrecht, Germany).
4. 2-mL reaction tubes (Sarstedt, Nu ¨mbrecht, Germany).
5. Sterile single-use pipette tips (Serological pipettes, Sarstedt,
Nu ¨mbrecht, Germany).
6. Pipette holder/rubber bulb (Pipetus, Hirschmann Laborger€ ate, Eberstadt, Germany).
7. 10-mL single-use syringes (B. Braun, Melsungen, Germany).
8. Single-use cannulas, blunt with Luer lock attachment
(B. Braun, Melsungen, Germany).
9. Luer lock syringe cap male/female (B. Braun, Melsungen,
Germany).
2.2.4 Performing
the SXC
1. 10-mL superloop (GE Healthcare Life Sciences, Uppsala,
Sweden)
2. FPLC system (e.g., A ¨ kta Pure 25; GE Healthcare Life Sciences,
Uppsala, Sweden).
3. Buffers prepared in Subheading 2.2.2.
4. 15-mL centrifuge tubes (Sarstedt, Nu ¨mbrecht, Germany).
5. Inline filter holder device 13 mm (Pall Life Sciences, Port
Washington, NY, USA).
228
Keven Lothert et al.