(c) Oxygen probe (VisiFerm DO, Hamilton, Bonaduz,
Switzerland).
(d) Biomass monitoring: online permittivity (ArcView,
Hamilton, Hoechst im Odenwald, Germany & Futura,
Aber Instruments, Aberystwyth, UK).
7. Water bath at 37
C (WNB 22, Memmert GmbH + Co. KG,
Schwabach, Germany).
8. Centrifuge tubes, 15- and 50-mL (Sarstedt, Nu ¨mbrecht,
Germany).
9. Silicon tubes 3–5 mm inner diameter (Carl Roth GmbH,
Karlsruhe, Germany).
10. Air filters, 0.2 μm poresize (Midisart 2000
® PTFE, Sartorius
AG, Goettingen, Germany).
11. Glass bottle with connector caps for base, harvest and bottom
drain (medium and inoculation).
12. Sterile syringe 10 mL, 20 mL (B. Braun, Melsungen,
Germany).
13. 15- and 50-mL centrifugation tubes (Sarstedt, Nu ¨mbrecht,
Germany).
14. 1.5 and 2 mL reaction tubes (Sarstedt, Nu ¨mbrecht, Germany).
15. Male and female luer lock adapter and lids (Sarstedt, Nu ¨mbrecht, Germany).
16. Y-tube connector (Bu ¨rkle GmbH, Bad Bellingen, Germany).
17. 1 M NaOH (Carl Roth GmbH, Karlsruhe, Germany)
18. Pressurized air, O 2 and CO 2 for aeration and pH control.
2.1.6 Cell Expansion
and Nanoplex Production
in the STR
1. Preculture of, for example, Vero cells (# CCL-81, ATCC,
Manassas, VA, USA).
2. Prewarmed (37
C) DMEM-HG growth medium, supplemented with 10% (v/v) FBS, 10 mM HEPES, and 4 mM L -glutamine (Biochrom, Berlin, Germany).
3. Sterile bottles (2 Â 0.5 L) with silicon tubes and Luer lock
adapters.
4. Microcarrier (Cytodex 1, GE Healthcare, Uppsala, Sweden).
5. Infectious recombinant Measles virus strain (e.g., MVvac2
GFP (P): Paul-Ehrlich-Institute, Langen, Germany).
6. 10-mL single-use syringes (B. Braun, Melsungen, Germany).
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Keven Lothert et al.
Switzerland).
(d) Biomass monitoring: online permittivity (ArcView,
Hamilton, Hoechst im Odenwald, Germany & Futura,
Aber Instruments, Aberystwyth, UK).
7. Water bath at 37
C (WNB 22, Memmert GmbH + Co. KG,
Schwabach, Germany).
8. Centrifuge tubes, 15- and 50-mL (Sarstedt, Nu ¨mbrecht,
Germany).
9. Silicon tubes 3–5 mm inner diameter (Carl Roth GmbH,
Karlsruhe, Germany).
10. Air filters, 0.2 μm poresize (Midisart 2000
® PTFE, Sartorius
AG, Goettingen, Germany).
11. Glass bottle with connector caps for base, harvest and bottom
drain (medium and inoculation).
12. Sterile syringe 10 mL, 20 mL (B. Braun, Melsungen,
Germany).
13. 15- and 50-mL centrifugation tubes (Sarstedt, Nu ¨mbrecht,
Germany).
14. 1.5 and 2 mL reaction tubes (Sarstedt, Nu ¨mbrecht, Germany).
15. Male and female luer lock adapter and lids (Sarstedt, Nu ¨mbrecht, Germany).
16. Y-tube connector (Bu ¨rkle GmbH, Bad Bellingen, Germany).
17. 1 M NaOH (Carl Roth GmbH, Karlsruhe, Germany)
18. Pressurized air, O 2 and CO 2 for aeration and pH control.
2.1.6 Cell Expansion
and Nanoplex Production
in the STR
1. Preculture of, for example, Vero cells (# CCL-81, ATCC,
Manassas, VA, USA).
2. Prewarmed (37
C) DMEM-HG growth medium, supplemented with 10% (v/v) FBS, 10 mM HEPES, and 4 mM L -glutamine (Biochrom, Berlin, Germany).
3. Sterile bottles (2 Â 0.5 L) with silicon tubes and Luer lock
adapters.
4. Microcarrier (Cytodex 1, GE Healthcare, Uppsala, Sweden).
5. Infectious recombinant Measles virus strain (e.g., MVvac2
GFP (P): Paul-Ehrlich-Institute, Langen, Germany).
6. 10-mL single-use syringes (B. Braun, Melsungen, Germany).
226
Keven Lothert et al.