7. Tissot AC, Renhofa R, Schmitz N, Cielens I,
Meijerink E, Ose V, Jennings GT, Saudan P,
Pumpens P, Bachmann MF (2010) Versatile
virus-like particle carrier for epitope based vaccines. PLoS One 5(3):e9809
8. Shishovs M, Rumnieks J, Diebolder C,
Jaudzems K, Andreas LB, Stanek J, Kazaks A,
Kotelovica S, Akopjana I, Pintacuda G, Koning
RI, Tars K (2016) Structure of AP205 coat
protein reveals circular permutation in ssRNA
bacteriophages.
J
Mol
Biol
428
(21):4267–4279
9. Brune KD, Leneghan DB, Brian IJ, Ishizuka
AS, Bachmann MF, Draper SJ, Biswas S,
Howarth M (2016) Plug-and-Display: decoration of virus-like particles via isopeptide bonds
for modular immunization. Sci Rep 6:19234
10. Deuschle U, Kammerer W, Gentz R, Bujard H
(1986) Promoters of Escherichia coli: a
hierarchy of in vivo strength indicates alternate
structures. EMBO J 5(11):2987–2994
11. Schmidt TG, Skerra A (2007) The Strep-tag
system for one-step purification and highaffinity detection or capturing of proteins. Nat
Protoc 2(6):1528–1535
12. Porterfield JZ, Zlotnick A (2010) A simple and
general method for determining the protein
and nucleic acid content of viruses by UV
absorbance. Virology 407(2):281–288
13. Kang D, Gho YS, Suh M, Kang C (2002)
Highly sensitive and fast protein detection
with coomassie brilliant blue in sodium dodecyl
sulfate-polyacrylamide gel electrophoresis. Bull
Kor Chem Soc 23(11):1511–1512
14. Sch€ agger H (2006) Tricine-SDS-PAGE. Nat
Protoc 1(1):16–22
Biosynthesis of Glycoconjugate VLPs
215