96-well plates. This involves the end-user specification of up to
eight Buffer A/B pairs (Fig. 1b), the duration of the gradients in
column volumes, the %B at the beginning and end of the gradients,
and the desired volume for preparing each buffer/step in the
gradients. Here, gradients for up to eight miniature columns are
prepared at a time by mixing together pairs from up to 16 buffer
stocks at different ratios in order to obtain the desired step compositions per gradient and column. In each of these pairs one stock
plays the role of Buffer A whereas the other the role of Buffer
B. Upon input definition, the tools result in the generation of
robotic instructions to prepare miniature column gradients in an
automated fashion through generic Tecan Freedom EVOware
scripts (see Notes 12 and 13).
Fig. 3 (a, b) Depiction of first 12 fraction collections using an automated liquid handler and the Te-Shuttle
module which ensures that fraction collection occurs in a different column of a Collection plate for each new
fraction
HTPD Purification of Viral Antigens
125