cells. Sensors (Basel) 18(3). https://doi.org/
10.3390/s18030900
6. Life Technologies (2015) Bac-to-Bac baculovirus expression system: an efficient site-specific
transposition system to generate baculovirus
for high-level expression of recombinant proteins. Life Technologies, Carlsbad, CA
7. Sung L-Y, Chen C-L, Lin S-Y et al (2014)
Efficient gene delivery into cell lines and stem
cells using baculovirus. Nat Protoc 9
(8):1882–1899. https://doi.org/10.1038/
nprot.2014.130
8. K€ aßer L, Zitzmann J, Grein TA et al (2019)
Turbidimetry and dielectric spectroscopy as
process analytical technologies for mammalian
and insect cell cultures. Methods Mol Biol
2095:335–364.
https://doi.org/10.1007/
978-1-0716-0191-4_20
9. Thermo Fisher Scientific PureLink Viral RNA
DNA Mini Kit User Guide (Pub.
No. MAN0000562 Rev. A.0)
10. €
Ustu ¨n-Aytekin O ¨ , Gu ¨rhan I ˙ D, Ohura K et al
(2013) Monitoring of the effects of transfection with baculovirus on Sf9 cell line and
expression of human dipeptidyl peptidase
IV. Cytotechnology 66(1):159–168. https://
doi.org/10.1007/s10616-013-9549-3
11. Folgueras-Flatschart AV, Flatschart RB,
Resende M et al (2000) Early detection of
productive baculovirus DNA transfection. Biotechniques 29(3):430–432., 434. https://doi.
org/10.2144/00293bm05
12. Wickham TJ, Davis T, Granados RR et al
(1991) Baculovirus defective interfering particles are responsible for variations in recombinant protein production as a function of
multiplicity of infection. Biotechnol Lett 13
(7):483–488.
https://doi.org/10.1007/
BF01049204
13. Licari P, Bailey JE (1991) Factors influencing
recombinant protein yields in an insect cellbacuiovirus expression system: multiplicity of
infection and intracellular protein degradation.
Biotechnol Bioeng 37(3):238–246. https://
doi.org/10.1002/bit.260370306
14. Maranga L, Braza ˜o TF, Carrondo MJT (2003)
Virus-like particle production at low multiplicities of infection with the baculovirus insect
cell
system.
Biotechnol
Bioeng
84
(2):245–253. https://doi.org/10.1002/bit.
10773
118
Julie Harnischfeger et al.
10.3390/s18030900
6. Life Technologies (2015) Bac-to-Bac baculovirus expression system: an efficient site-specific
transposition system to generate baculovirus
for high-level expression of recombinant proteins. Life Technologies, Carlsbad, CA
7. Sung L-Y, Chen C-L, Lin S-Y et al (2014)
Efficient gene delivery into cell lines and stem
cells using baculovirus. Nat Protoc 9
(8):1882–1899. https://doi.org/10.1038/
nprot.2014.130
8. K€ aßer L, Zitzmann J, Grein TA et al (2019)
Turbidimetry and dielectric spectroscopy as
process analytical technologies for mammalian
and insect cell cultures. Methods Mol Biol
2095:335–364.
https://doi.org/10.1007/
978-1-0716-0191-4_20
9. Thermo Fisher Scientific PureLink Viral RNA
DNA Mini Kit User Guide (Pub.
No. MAN0000562 Rev. A.0)
10. €
Ustu ¨n-Aytekin O ¨ , Gu ¨rhan I ˙ D, Ohura K et al
(2013) Monitoring of the effects of transfection with baculovirus on Sf9 cell line and
expression of human dipeptidyl peptidase
IV. Cytotechnology 66(1):159–168. https://
doi.org/10.1007/s10616-013-9549-3
11. Folgueras-Flatschart AV, Flatschart RB,
Resende M et al (2000) Early detection of
productive baculovirus DNA transfection. Biotechniques 29(3):430–432., 434. https://doi.
org/10.2144/00293bm05
12. Wickham TJ, Davis T, Granados RR et al
(1991) Baculovirus defective interfering particles are responsible for variations in recombinant protein production as a function of
multiplicity of infection. Biotechnol Lett 13
(7):483–488.
https://doi.org/10.1007/
BF01049204
13. Licari P, Bailey JE (1991) Factors influencing
recombinant protein yields in an insect cellbacuiovirus expression system: multiplicity of
infection and intracellular protein degradation.
Biotechnol Bioeng 37(3):238–246. https://
doi.org/10.1002/bit.260370306
14. Maranga L, Braza ˜o TF, Carrondo MJT (2003)
Virus-like particle production at low multiplicities of infection with the baculovirus insect
cell
system.
Biotechnol
Bioeng
84
(2):245–253. https://doi.org/10.1002/bit.
10773
118
Julie Harnischfeger et al.