11. Thermomixer (e.g., Thermomixer comfort, Eppendorf, Hamburg, Germany).
12. Incubator (e.g., INCU-Line
® IL 23, VWR, Radnor,
Pennsylvania, USA).
2.2.2 Materials
for Analysis
of the Recombinant Bacmid
Using LongAmp Taq
Polymerase
1. MAX Efficiency
® DH10-Bac™ colonies (e.g., Bac-to-Bac™
Baculovirus Expression System, Thermo Fisher Scientific, Waltham, MA, USA).
2. 1.5 mL centrifuge tubes (e.g., Sarstedt, Nu ¨mbrecht,
Germany).
3. 5Â LongAmp Taq Polymerase (e.g., New England Biolabs,
Ipswich, Massachusetts, USA).
4. LongAmp Taq Reaction buffer (e.g., New England Biolabs,
Ipswich, Massachusetts, USA).
5. Deoxyribonucleoside triphosphates (e.g., Deoxyribonucleoside (dNTP) Solution Mix, New England Biolabs, Ipswich,
Massachusetts, USA).
6. M13 forward (-40) primer and M13 reverse primer (Source:
e.g., Biomers.de, Ulm, Germany; Synthesis: e.g., SigmaAldrich, St. Louis, Missouri, USA).
7. Distilled water, cell-culture grade (e.g., Milli-Q Academic,
Millipore, Burlington, Massachusetts, USA).
8. Sterile single-use pipette tips (e.g., pipette tips, Sarstedt, Nu ¨mbrecht, Germany).
9. Stained agarose gel (1% (w/v) agarose with for example SYBR
Safe DNA Gel Stain, Invitrogen, Carlsbad, California, USA).
10. DNA loading buffer (e.g., Gel Loading Dye, Purple (6Â), New
England Biolabs, Ipswich, Massachusetts, USA).
11. DNA ladder (e.g., 2 Log DNA Ladder, New England Biolabs,
Ipswich, Massachusetts, USA).
12. PCR tubes (e.g., Strip tubes for PCR, Thermo Fisher Scientific, Waltham, MA, USA).
13. Piston pipettes (e.g., Research Plus, Eppendorf, Hamburg,
Germany).
14. Single-use pipette tips (e.g., pipette tips, Sarstedt, Nu ¨mbrecht,
Germany).
15. Thermocycler
(e.g.,
peqSTAR,
VWR,
Radnor,
Pennsylvania, USA).
16. Electrophoresis chamber (e.g., Biozym Scientific, Hessisch
Oldendorf, Germany).
17. Gel imager (e.g., ChemiDoc™XRS+ Imager, Bio-Rad Laboratories, Hercules, California, USA).
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