Acknowledgments
The research leading to these results has received funding from the
EEA Grants 2014-2021, within the GreenVac and SmartVac Projects, contracts no. 5/2014 and no. 1/2019.
References
1. Dalton AC, Barton WA (2014) Overexpression of secreted proteins from mammalian cell lines. Protein Sci 23(5):517–525
2. Herscovics A, Orlean P (1993) Glycoprotein
biosynthesis in yeast. FASEB J 7(6):540–550
3. Altmann F, Staudacher E, Wilson IB, Marz L
(1999) Insect cells as hosts for the expression
of recombinant glycoproteins. Glycoconj J 16
(2):109–123
4. Siegler VD, Bruss V (2013) Role of transmembrane domains of hepatitis B virus small surface
proteins in subviral-particle biogenesis. J Virol
87(3):1491–1496
5. Gilbert RJ, Beales L, Blond D, Simon MN, Lin
BY, Chisari FV, Stuart DI, Rowlands DJ
(2005) Hepatitis B small surface antigen particles are octahedral. Proc Natl Acad Sci U S A
102(41):14783–14788
6. Komatsu H (2014) Hepatitis B virus: where do
we stand and what is the next step for eradication?
World
J
Gastroenterol
20
(27):8998–9016
7. Lee IH, Kim CH, Ryu WS (1996) Presentation
of the hydrophilic domains of hepatitis C viral
E2 envelope glycoprotein on hepatitis B surface antigen particles. J Med Virol 50
(2):145–151
8. Netter HJ, Macnaughton TB, Woo WP,
Tindle R, Gowans EJ (2001) Antigenicity and
immunogenicity of novel chimeric hepatitis B
surface antigen particles with exposed hepatitis
C virus epitopes. J Virol 75(5):2130–2141
9. Patient R, Hourioux C, Vaudin P, Pages JC,
Roingeard P (2009) Chimeric hepatitis B and
C viruses envelope proteins can form subviral
particles: implications for the design of new
vaccine strategies. New Biotechnol 25
(4):226–234
10. Dobrica MO, Lazar C, Paruch L, Skomedal H,
Steen H, Haugslien S, Tucureanu C, Caras I,
Onu A, Ciulean S, Branzan A, Clarke JL,
Stavaru C, Branza-Nichita N (2017) A novel
chimeric Hepatitis B virus S/preS1 antigen
produced in mammalian and plant cells elicits
stronger humoral and cellular immune
response than the standard vaccineconstituent,
S
protein.
Antivir
Res
144:256–265
11. Dobrica MO, Lazar C, Paruch L, van Eerde A,
Clarke JL, Tucureanu C, Caras I, Ciulean S,
Onu A, Tofan V, Branzan A, Urban S,
Stavaru C, Branza-Nichita N (2018) Oral
administration of a chimeric Hepatitis B Virus
S/preS1 antigen produced in lettuce triggers
infection neutralizing antibodies in mice. Vaccine 36(38):5789–5795
12. Beaumont E, Roingeard P (2015) Chimeric
hepatitis B virus (HBV)/hepatitis C virus
(HCV) subviral envelope particles induce efficient anti-HCV antibody production in animals
pre-immunized with HBV vaccine. Vaccine 33
(8):973–976
Open Access This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International
License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution
and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the
source, provide a link to the Creative Commons license and indicate if changes were made.
The images or other third party material in this chapter are included in the chapter’s Creative Commons license,
unless indicated otherwise in a credit line to the material. If material is not included in the chapter’s Creative
Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use,
you will need to obtain permission directly from the copyright holder.
94
Mihaela-Olivia Dobrica et al.
The research leading to these results has received funding from the
EEA Grants 2014-2021, within the GreenVac and SmartVac Projects, contracts no. 5/2014 and no. 1/2019.
References
1. Dalton AC, Barton WA (2014) Overexpression of secreted proteins from mammalian cell lines. Protein Sci 23(5):517–525
2. Herscovics A, Orlean P (1993) Glycoprotein
biosynthesis in yeast. FASEB J 7(6):540–550
3. Altmann F, Staudacher E, Wilson IB, Marz L
(1999) Insect cells as hosts for the expression
of recombinant glycoproteins. Glycoconj J 16
(2):109–123
4. Siegler VD, Bruss V (2013) Role of transmembrane domains of hepatitis B virus small surface
proteins in subviral-particle biogenesis. J Virol
87(3):1491–1496
5. Gilbert RJ, Beales L, Blond D, Simon MN, Lin
BY, Chisari FV, Stuart DI, Rowlands DJ
(2005) Hepatitis B small surface antigen particles are octahedral. Proc Natl Acad Sci U S A
102(41):14783–14788
6. Komatsu H (2014) Hepatitis B virus: where do
we stand and what is the next step for eradication?
World
J
Gastroenterol
20
(27):8998–9016
7. Lee IH, Kim CH, Ryu WS (1996) Presentation
of the hydrophilic domains of hepatitis C viral
E2 envelope glycoprotein on hepatitis B surface antigen particles. J Med Virol 50
(2):145–151
8. Netter HJ, Macnaughton TB, Woo WP,
Tindle R, Gowans EJ (2001) Antigenicity and
immunogenicity of novel chimeric hepatitis B
surface antigen particles with exposed hepatitis
C virus epitopes. J Virol 75(5):2130–2141
9. Patient R, Hourioux C, Vaudin P, Pages JC,
Roingeard P (2009) Chimeric hepatitis B and
C viruses envelope proteins can form subviral
particles: implications for the design of new
vaccine strategies. New Biotechnol 25
(4):226–234
10. Dobrica MO, Lazar C, Paruch L, Skomedal H,
Steen H, Haugslien S, Tucureanu C, Caras I,
Onu A, Ciulean S, Branzan A, Clarke JL,
Stavaru C, Branza-Nichita N (2017) A novel
chimeric Hepatitis B virus S/preS1 antigen
produced in mammalian and plant cells elicits
stronger humoral and cellular immune
response than the standard vaccineconstituent,
S
protein.
Antivir
Res
144:256–265
11. Dobrica MO, Lazar C, Paruch L, van Eerde A,
Clarke JL, Tucureanu C, Caras I, Ciulean S,
Onu A, Tofan V, Branzan A, Urban S,
Stavaru C, Branza-Nichita N (2018) Oral
administration of a chimeric Hepatitis B Virus
S/preS1 antigen produced in lettuce triggers
infection neutralizing antibodies in mice. Vaccine 36(38):5789–5795
12. Beaumont E, Roingeard P (2015) Chimeric
hepatitis B virus (HBV)/hepatitis C virus
(HCV) subviral envelope particles induce efficient anti-HCV antibody production in animals
pre-immunized with HBV vaccine. Vaccine 33
(8):973–976
Open Access This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International
License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution
and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the
source, provide a link to the Creative Commons license and indicate if changes were made.
The images or other third party material in this chapter are included in the chapter’s Creative Commons license,
unless indicated otherwise in a credit line to the material. If material is not included in the chapter’s Creative
Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use,
you will need to obtain permission directly from the copyright holder.
94
Mihaela-Olivia Dobrica et al.