98
water- material ratio 30 (mL:g), and the greater recovery of A. bisporus polysaccharide was obtained at 6.02% (Tian et al. 2012). Moreover, the yield of polysaccharide
from L. edodes has been determined under the conditions of ultrasound power
340 W, water-material ratio 30:1 (mL:g), and ultrasound time 14 min was 14.39%,
which is significantly higher than water heating extraction (Ke 2014).
However, the utilization of ultrasounds for β-glucan extraction is very limited in
the literature. Izydorczyk et al. (2000) have employed sonication for physical disruption before the extraction (pre-treatment) showing that this pretreatment will
Grinding and sieving barley flour
Refluxing with ethanol 80 % (v/v) (3 h.)
Drying in oven at 40°C for 12 h.
Suspending in double distillated water at 50 °C (1:10 w/v)
Adjusting solution pH (5-9)
Ultrasound assisted extraction at 50 °C (amplitude:50-100, sonication time: 3-7 min)
Centrifuging at 15000 g for 10 min at 20 °C
Adjusting supernatant pH at 4 with HCI
Centrifuging at 20000 g for 20 min at 4 °C
Precipitate
Precipitate
Adjusting supernatant pH at 7 with NaOH
Mixing supernatant with ethanol 99 % (v/v)and kept overnight at 4°C
Filtering β-glucan and drying by freeze dryer
Fig. 1 Schematic outline for UAE method and purification procedures of barley β-D-glucan
N. Jan et al.
water- material ratio 30 (mL:g), and the greater recovery of A. bisporus polysaccharide was obtained at 6.02% (Tian et al. 2012). Moreover, the yield of polysaccharide
from L. edodes has been determined under the conditions of ultrasound power
340 W, water-material ratio 30:1 (mL:g), and ultrasound time 14 min was 14.39%,
which is significantly higher than water heating extraction (Ke 2014).
However, the utilization of ultrasounds for β-glucan extraction is very limited in
the literature. Izydorczyk et al. (2000) have employed sonication for physical disruption before the extraction (pre-treatment) showing that this pretreatment will
Grinding and sieving barley flour
Refluxing with ethanol 80 % (v/v) (3 h.)
Drying in oven at 40°C for 12 h.
Suspending in double distillated water at 50 °C (1:10 w/v)
Adjusting solution pH (5-9)
Ultrasound assisted extraction at 50 °C (amplitude:50-100, sonication time: 3-7 min)
Centrifuging at 15000 g for 10 min at 20 °C
Adjusting supernatant pH at 4 with HCI
Centrifuging at 20000 g for 20 min at 4 °C
Precipitate
Precipitate
Adjusting supernatant pH at 7 with NaOH
Mixing supernatant with ethanol 99 % (v/v)and kept overnight at 4°C
Filtering β-glucan and drying by freeze dryer
Fig. 1 Schematic outline for UAE method and purification procedures of barley β-D-glucan
N. Jan et al.