11. We recommend to carefully aspirate as much supernatant as
possible, to avoid diluting the bioink.
12. We recommend the following parameters to achieve the best
printing results: layer thickness ¼ 100 μm, printing
speed ¼ 235 mm/min, and fiber-to-fiber distance ¼ 400 μm.
13. According to the UV light intensity generated by the source
(generally a UV lamp), the crosslinking time may change. A
low-dose UV irradiation of 1.3 mW/cm
2 for 5 min at 365 nm
should be enough to crosslink the hydrogel while still not
affecting cell viability.
14. Alternatively, fixation can be performed in ice cold methanol
for 1 h at 4
C.
References
1. Gibson I, Rosen D, Stucker B (2015) Additive
manufacturing
technologies.
Springer,
New York, NY
2. Murphy SV, Atala A (2014) 3D bioprinting of
tissues and organs. Nat Biotechnol 32:773
3. Kang HW, Lee SJ, Ko IK, Kengla C, Yoo JJ,
Atala A (2016) A 3D bioprinting system to
produce human-scale tissue constructs with
structural
integrity.
Nat
Biotechnol
34:312–319
4. Ji S, Guvendiren M (2015) Recent advances in
bioink design for 3D bioprinting of tissues and
organs. Front Bioeng Biotechnol 5:23–31
5. Ho ¨lzl K, Lin S, Tytgat L, Van Vlierberghe S,
Gu L, Ovsianikov A (2016) Bioink properties
before, during and after 3D bioprinting. Biofabrication 8:32002
6. Chimene D, Lennox KK, Kaunas RR, Gaharwar AK (2016) Advanced bioinks for 3D printing: a materials science perspective. Ann
Biomed Eng 44:2090–2102
7. Panwar A, Tan L (2016) Current status of
bioinks for micro-extrusion-based 3D bioprinting. Molecules 21:E685
8. Armstrong JPK, Burke M, Carter BM, Davis
SA, Perriman AW (2016) 3D bioprinting using
a templated porous bioink. Adv Healthc Mater
5:1724
9. Axpe E, Oyen ML (2016) Applications of
alginate-based bioinks in 3D bioprinting. Int
J Mol Sci 17:1976
10. Xu T, Zhao W, Zhu JM, Albanna MZ, Yoo JJ,
Atala A (2013) Complex heterogeneous tissue
constructs containing multiple cell types
prepared by inkjet printing technology. Biomaterials 34:130–139
11. Xu T, Baicu C, Aho M, Zile M, Boland T
(2009) Fabrication and characterization of
bio-engineered cardiac pseudo tissues. Biofabrication 1:35001
12. Kosik-Kozioł A, Costantini M, Bolek T,
Szoke K, Barbetta A, Brinchmann JE, S ´ wie ˛szkowski W (2017) PLA short sub-micron fibers
reinforcement of 3D bioprinted alginate constructs for cartilage regeneration. Biofabrication 9:044105
13. Yeo MG, Lee JS, Chun W, Kim GH (2016) An
innovative collagen-based cell-printing method
for obtaining human adipose stem cell-laden
structures consisting of core-sheath structures
for tissue engineering. Biomacromolecules
17:1365–1375
14. Nishiyama Y, Nakamura M, Henmi C,
Yamaguchi K, Mochizuki S, Nakagawa H,
Takiura K (2007) Fabrication of 3D cell supporting structures with multi-materials using
the bio-printer. In: ASME 2007 International
manufacturing science and engineering conference, american society of mechanical engineers,
New York City
15. Khalil S, Sun W (2009) Bioprinting endothelial
cells with alginate for 3D tissue constructs. J
Biomech Eng 131:111002
16. Diogo GS, Gaspar VM, Serra IR, Fradique R,
Correia IJ (2014) Manufacture of β- TCP/alginate scaffolds through a Fab@home model
for application in bone tissue engineering. Biofabrication 6:25001
17. Costantini M, Idaszek J, Szo ¨ke K,
Jaroszewicz J, Dentini M, Barbetta A, Brinchmann JE, S ´ wie ˛szkowski W (2016) 3D bioprinting of BM-MSCs-loaded ECM biomimetic
hydrogels for in vitro neocartilage formation.
Biofabrication 8:35002
Photocurable Biopolymers for Coaxial Bioprinting
53
possible, to avoid diluting the bioink.
12. We recommend the following parameters to achieve the best
printing results: layer thickness ¼ 100 μm, printing
speed ¼ 235 mm/min, and fiber-to-fiber distance ¼ 400 μm.
13. According to the UV light intensity generated by the source
(generally a UV lamp), the crosslinking time may change. A
low-dose UV irradiation of 1.3 mW/cm
2 for 5 min at 365 nm
should be enough to crosslink the hydrogel while still not
affecting cell viability.
14. Alternatively, fixation can be performed in ice cold methanol
for 1 h at 4
C.
References
1. Gibson I, Rosen D, Stucker B (2015) Additive
manufacturing
technologies.
Springer,
New York, NY
2. Murphy SV, Atala A (2014) 3D bioprinting of
tissues and organs. Nat Biotechnol 32:773
3. Kang HW, Lee SJ, Ko IK, Kengla C, Yoo JJ,
Atala A (2016) A 3D bioprinting system to
produce human-scale tissue constructs with
structural
integrity.
Nat
Biotechnol
34:312–319
4. Ji S, Guvendiren M (2015) Recent advances in
bioink design for 3D bioprinting of tissues and
organs. Front Bioeng Biotechnol 5:23–31
5. Ho ¨lzl K, Lin S, Tytgat L, Van Vlierberghe S,
Gu L, Ovsianikov A (2016) Bioink properties
before, during and after 3D bioprinting. Biofabrication 8:32002
6. Chimene D, Lennox KK, Kaunas RR, Gaharwar AK (2016) Advanced bioinks for 3D printing: a materials science perspective. Ann
Biomed Eng 44:2090–2102
7. Panwar A, Tan L (2016) Current status of
bioinks for micro-extrusion-based 3D bioprinting. Molecules 21:E685
8. Armstrong JPK, Burke M, Carter BM, Davis
SA, Perriman AW (2016) 3D bioprinting using
a templated porous bioink. Adv Healthc Mater
5:1724
9. Axpe E, Oyen ML (2016) Applications of
alginate-based bioinks in 3D bioprinting. Int
J Mol Sci 17:1976
10. Xu T, Zhao W, Zhu JM, Albanna MZ, Yoo JJ,
Atala A (2013) Complex heterogeneous tissue
constructs containing multiple cell types
prepared by inkjet printing technology. Biomaterials 34:130–139
11. Xu T, Baicu C, Aho M, Zile M, Boland T
(2009) Fabrication and characterization of
bio-engineered cardiac pseudo tissues. Biofabrication 1:35001
12. Kosik-Kozioł A, Costantini M, Bolek T,
Szoke K, Barbetta A, Brinchmann JE, S ´ wie ˛szkowski W (2017) PLA short sub-micron fibers
reinforcement of 3D bioprinted alginate constructs for cartilage regeneration. Biofabrication 9:044105
13. Yeo MG, Lee JS, Chun W, Kim GH (2016) An
innovative collagen-based cell-printing method
for obtaining human adipose stem cell-laden
structures consisting of core-sheath structures
for tissue engineering. Biomacromolecules
17:1365–1375
14. Nishiyama Y, Nakamura M, Henmi C,
Yamaguchi K, Mochizuki S, Nakagawa H,
Takiura K (2007) Fabrication of 3D cell supporting structures with multi-materials using
the bio-printer. In: ASME 2007 International
manufacturing science and engineering conference, american society of mechanical engineers,
New York City
15. Khalil S, Sun W (2009) Bioprinting endothelial
cells with alginate for 3D tissue constructs. J
Biomech Eng 131:111002
16. Diogo GS, Gaspar VM, Serra IR, Fradique R,
Correia IJ (2014) Manufacture of β- TCP/alginate scaffolds through a Fab@home model
for application in bone tissue engineering. Biofabrication 6:25001
17. Costantini M, Idaszek J, Szo ¨ke K,
Jaroszewicz J, Dentini M, Barbetta A, Brinchmann JE, S ´ wie ˛szkowski W (2016) 3D bioprinting of BM-MSCs-loaded ECM biomimetic
hydrogels for in vitro neocartilage formation.
Biofabrication 8:35002
Photocurable Biopolymers for Coaxial Bioprinting
53