6. Allow the mixture to cool down to room temperature and rest
overnight.
7. Filter the resulting mixture and use 2-butanone to wash the
filtrate three times to remove the unreacted lithium bromide.
8. Remove the excess solvent by vacuum and leave LAP in a white
solid chunk state.
9. Pestle the chunk LAP into powder and store it at À80
C under
argon for future use.
3.4 Cell and Tissue
Cultures
1. Culture HUVECs in EGM-2 according to the protocol from
the vendor (see Note 10).
2. Culture 10T1/2 s in DMEM supplemented with 10% FBS
according to the protocol from the vendor.
3. Culture the bioprinted tissue constructs in EGM-2 (see Note
11).
3.5 μCOB
System Setup
1. The schematic of the μCOB is shown in Fig. 1.
2. The DMD chip is used to dictate the optical pattern projected
onto the prepolymer solution on the fabrication stage.
Fig. 1 Schematic of the μCOB platform (adapted from [17])
Bioprinting of Complex Vascularized Tissues
167
overnight.
7. Filter the resulting mixture and use 2-butanone to wash the
filtrate three times to remove the unreacted lithium bromide.
8. Remove the excess solvent by vacuum and leave LAP in a white
solid chunk state.
9. Pestle the chunk LAP into powder and store it at À80
C under
argon for future use.
3.4 Cell and Tissue
Cultures
1. Culture HUVECs in EGM-2 according to the protocol from
the vendor (see Note 10).
2. Culture 10T1/2 s in DMEM supplemented with 10% FBS
according to the protocol from the vendor.
3. Culture the bioprinted tissue constructs in EGM-2 (see Note
11).
3.5 μCOB
System Setup
1. The schematic of the μCOB is shown in Fig. 1.
2. The DMD chip is used to dictate the optical pattern projected
onto the prepolymer solution on the fabrication stage.
Fig. 1 Schematic of the μCOB platform (adapted from [17])
Bioprinting of Complex Vascularized Tissues
167