5. The time necessary to induce bioink gelation is generally
between 10 and 15 min.
6. Depending on the rheological properties of the printing solution, the extrusion pressure can be varied between 1 and 3 bar.
7. Pluronic F127 is rapidly eluted from the constructs during the
first hours in culture and can be removed by frequent medium
changes.
Acknowledgment
This work was partially supported by FIRB 2010 Project “Bioartificial materials and biomimetic scaffolds for a stem cells-based
therapy for myocardial regeneration” (grant no. RBFR10L0GK)
financed by MIUR (Italian Ministry of Education University and
Research).
References
1. Ozbolat IT (2015) Bioprinting scale-up tissue
and organ constructs for transplantation.
Trends Biotechnol 33:395–400. https://doi.
org/10.1016/j.tibtech.2015.04.005
2. Zhao Y, Yao R, Ouyang L et al (2014) Threedimensional printing of Hela cells for cervical
tumor model in vitro. Biofabrication 6:35001.
https://doi.org/10.1088/1758-5082/6/3/
035001
3. Vanderburgh J, Sterling JA, Guelcher SA
(2017) 3D printing of tissue engineered constructs for in vitro modeling of disease progression and drug screening. Ann Biomed Eng
45:164–179.
https://doi.org/10.1007/
s10439-016-1640-4
4. Yoo S-S (2009) 3-dimensional multi-layered
hydrogels and methods of making the same,
US Patent 13/063,502
5. Hasan SK (2009) Three dimensional tissue
generation, US Patent 13/130,740
6. Melchels FPW, Klein TJ, Malda J et al (2012)
Additive manufacturing of tissues and organs.
Prog Polym Sci 37:1079–1104. https://doi.
org/10.1016/J.PROGPOLYMSCI.2011.11.
007
7. Chiono V, Sartori S, Boffito M et al (2016)
Method for preparing cellularized constructs
based on heat-sensitive hydro-gels. Italian Patent IT102015000020718
8. Gioffredi E, Boffito M, Calzone S et al (2016)
Pluronic F127 hydrogel characterization and
biofabrication in cellularized constructs for tissue engineering applications. Procedia CIRP
49:125–132.
https://doi.org/10.1016/j.
procir.2015.11.001
9. Mozetic P, Giannitelli SM, Gori M et al (2017)
Engineering muscle cell alignment through 3D
bioprinting. J Biomed Mater Res A
105:2582–2588. https://doi.org/10.1002/
jbm.a.36117
10. Giannitelli SM, Mozetic P, Trombetta M et al
(2015) Additive manufacturing of pluronic/
alginate composite thermogels for drug and
cell delivery. In: Srivatsan TS, Sudarshan TS
(eds) Additive manufacturing: innovations,
advances and applications. CRC Press, Boca
Raton
11. Schmolka IR (1977) A review of block polymer
surfactants. J Am Oil Chem Soc 54:110–116.
https://doi.org/10.1007/BF02894385
12. Rainer A, Giannitelli SM, Accoto D et al
(2012) Load-adaptive scaffold architecturing:
a bioinspired approach to the design of porous
additively manufactured scaffolds with optimized mechanical properties. Ann Biomed
Eng 40:966–975. https://doi.org/10.1007/
s10439-011-0465-4
142
Sara Maria Giannitelli et al.
between 10 and 15 min.
6. Depending on the rheological properties of the printing solution, the extrusion pressure can be varied between 1 and 3 bar.
7. Pluronic F127 is rapidly eluted from the constructs during the
first hours in culture and can be removed by frequent medium
changes.
Acknowledgment
This work was partially supported by FIRB 2010 Project “Bioartificial materials and biomimetic scaffolds for a stem cells-based
therapy for myocardial regeneration” (grant no. RBFR10L0GK)
financed by MIUR (Italian Ministry of Education University and
Research).
References
1. Ozbolat IT (2015) Bioprinting scale-up tissue
and organ constructs for transplantation.
Trends Biotechnol 33:395–400. https://doi.
org/10.1016/j.tibtech.2015.04.005
2. Zhao Y, Yao R, Ouyang L et al (2014) Threedimensional printing of Hela cells for cervical
tumor model in vitro. Biofabrication 6:35001.
https://doi.org/10.1088/1758-5082/6/3/
035001
3. Vanderburgh J, Sterling JA, Guelcher SA
(2017) 3D printing of tissue engineered constructs for in vitro modeling of disease progression and drug screening. Ann Biomed Eng
45:164–179.
https://doi.org/10.1007/
s10439-016-1640-4
4. Yoo S-S (2009) 3-dimensional multi-layered
hydrogels and methods of making the same,
US Patent 13/063,502
5. Hasan SK (2009) Three dimensional tissue
generation, US Patent 13/130,740
6. Melchels FPW, Klein TJ, Malda J et al (2012)
Additive manufacturing of tissues and organs.
Prog Polym Sci 37:1079–1104. https://doi.
org/10.1016/J.PROGPOLYMSCI.2011.11.
007
7. Chiono V, Sartori S, Boffito M et al (2016)
Method for preparing cellularized constructs
based on heat-sensitive hydro-gels. Italian Patent IT102015000020718
8. Gioffredi E, Boffito M, Calzone S et al (2016)
Pluronic F127 hydrogel characterization and
biofabrication in cellularized constructs for tissue engineering applications. Procedia CIRP
49:125–132.
https://doi.org/10.1016/j.
procir.2015.11.001
9. Mozetic P, Giannitelli SM, Gori M et al (2017)
Engineering muscle cell alignment through 3D
bioprinting. J Biomed Mater Res A
105:2582–2588. https://doi.org/10.1002/
jbm.a.36117
10. Giannitelli SM, Mozetic P, Trombetta M et al
(2015) Additive manufacturing of pluronic/
alginate composite thermogels for drug and
cell delivery. In: Srivatsan TS, Sudarshan TS
(eds) Additive manufacturing: innovations,
advances and applications. CRC Press, Boca
Raton
11. Schmolka IR (1977) A review of block polymer
surfactants. J Am Oil Chem Soc 54:110–116.
https://doi.org/10.1007/BF02894385
12. Rainer A, Giannitelli SM, Accoto D et al
(2012) Load-adaptive scaffold architecturing:
a bioinspired approach to the design of porous
additively manufactured scaffolds with optimized mechanical properties. Ann Biomed
Eng 40:966–975. https://doi.org/10.1007/
s10439-011-0465-4
142
Sara Maria Giannitelli et al.