554
O. Lavrynenko et al.
to 25 A · m
2 · kg
−1 when the initial argentum’s concentration was increased from
0.5 to 5.0 mg · dm
−3 [8]. But such a trend is not confirmed for Fe 3 O 4 and Au
0
nanocomposites.
So, analysis of the results of magnetic study let to confirm the association of
relative high saturation magnetization for the nanopowders formed under conditions
when magnetite was formed as a dominated phase. The increase in the lepidocrocite
part in the phase composition of the surface structures caused a decrease in the values
of M s .
3.5 Biomedical Application of Magnetite Nanoparticles
Doped with Argentum and Aurum
As prepared sols of nanocomposites based on magnetite doped with argentum
obtained when the St3 surface was contacting with AgNO 3 water solutions at c(Ag
+ )
0.5 and 5 mg/dm
3 were used to study of the lipid peroxygenation in the lecithincontaining model system under the conditions of oxidative stress. The choice of
such composites was conditional on the well-known antimicrobial and antifungal
activity of silver and capability of argentum species to interact with cell membranes
via toxin inactivation. So, the nanoparticles FeFe 2 O 4 and Ag
0 were added into an
incubating medium containing 1.5 mg/ml of lecithin dissolved in 20 mM Tris-HCl
buffer at pH value 7.4. Incubation was performed in 1 h at T = 37 °C. The content of
lipid peroxyl radicals was measured by chemoluminescence enhanced by luminol.
The results show an insignificant decreasing of the chemoluminescence intensity of
the composite particles in comparison with nyllovo ppobo due to the antioxidative activity of silver. Our preliminary investigation permits mare conclusion about
the perspective of FeFe 2 O 4 and Ag
0 nanocomposite sol’s application to target supply
of pharmaceuticals and therapy of various pathological states accompanied by the
oxidative injuries of cells and bio-tissues.
The testing of antimicrobial activity of Fe 3 O 4 and Ag
0 nanoparticles was
performed for Gram-positive and Gram-negative bacterial cultures S. Aureus and E.
Coli containing 4 × 10
5 CFU. Incubation lasted 24 h at T = 37 °C. The results showed
the effectiveness of composite particles with respect to both kinds of species. Hence,
the composite structures based on magnetite and silver were admitted as viable to
the creation of new medical products directed on the medical treatment of infectious
complications of the patients of resuscitation and intensive care units, especially at
the development of nosocomial diseases.
At the same time, the core and shell type nanocomposite sols obtained via RCD
method are capable to initiate the lipid peroxygenation in the phosphatide dispersions
that accompanied by the burst of chemoluminescence even when EDTA overmeasure
was added into water medium. Because the appending of chelating agents decreased
the free radical generation, the obvious mechanism of the interaction in the system
O. Lavrynenko et al.
to 25 A · m
2 · kg
−1 when the initial argentum’s concentration was increased from
0.5 to 5.0 mg · dm
−3 [8]. But such a trend is not confirmed for Fe 3 O 4 and Au
0
nanocomposites.
So, analysis of the results of magnetic study let to confirm the association of
relative high saturation magnetization for the nanopowders formed under conditions
when magnetite was formed as a dominated phase. The increase in the lepidocrocite
part in the phase composition of the surface structures caused a decrease in the values
of M s .
3.5 Biomedical Application of Magnetite Nanoparticles
Doped with Argentum and Aurum
As prepared sols of nanocomposites based on magnetite doped with argentum
obtained when the St3 surface was contacting with AgNO 3 water solutions at c(Ag
+ )
0.5 and 5 mg/dm
3 were used to study of the lipid peroxygenation in the lecithincontaining model system under the conditions of oxidative stress. The choice of
such composites was conditional on the well-known antimicrobial and antifungal
activity of silver and capability of argentum species to interact with cell membranes
via toxin inactivation. So, the nanoparticles FeFe 2 O 4 and Ag
0 were added into an
incubating medium containing 1.5 mg/ml of lecithin dissolved in 20 mM Tris-HCl
buffer at pH value 7.4. Incubation was performed in 1 h at T = 37 °C. The content of
lipid peroxyl radicals was measured by chemoluminescence enhanced by luminol.
The results show an insignificant decreasing of the chemoluminescence intensity of
the composite particles in comparison with nyllovo ppobo due to the antioxidative activity of silver. Our preliminary investigation permits mare conclusion about
the perspective of FeFe 2 O 4 and Ag
0 nanocomposite sol’s application to target supply
of pharmaceuticals and therapy of various pathological states accompanied by the
oxidative injuries of cells and bio-tissues.
The testing of antimicrobial activity of Fe 3 O 4 and Ag
0 nanoparticles was
performed for Gram-positive and Gram-negative bacterial cultures S. Aureus and E.
Coli containing 4 × 10
5 CFU. Incubation lasted 24 h at T = 37 °C. The results showed
the effectiveness of composite particles with respect to both kinds of species. Hence,
the composite structures based on magnetite and silver were admitted as viable to
the creation of new medical products directed on the medical treatment of infectious
complications of the patients of resuscitation and intensive care units, especially at
the development of nosocomial diseases.
At the same time, the core and shell type nanocomposite sols obtained via RCD
method are capable to initiate the lipid peroxygenation in the phosphatide dispersions
that accompanied by the burst of chemoluminescence even when EDTA overmeasure
was added into water medium. Because the appending of chelating agents decreased
the free radical generation, the obvious mechanism of the interaction in the system