Chapter 6
Fabrication of Robust Biomolecular
Patterns by Reactive Microcontact
Printing on NHS Ester Containing
Polymer Films
In this chapter, the fabrication of robust biomolecule microarrays by reactive microcontact printing (μCP) on spin-coated thin films of poly(N-hydroxysuccinimidylmethacrylate) (PNHSMA) on oxidized silicon and glass is described. The approach
combines the advantages of activated polymer thin films as coupling layers, characterized by high reactivity and high molecular loading (see Chaps. 3 and 4), with the
versatility and flexibility of soft lithography. In this chapter, the fabrication of robust
biomolecule microarrays by reactive microcontact printing (μCP) on spin-coated thin
films of poly(N-hydroxysuccinimidyl-methacrylate) (PNHSMA) on oxidized silicon
and glass is described. The approach combines the advantages of activated polymer
thin films as coupling layers, characterized by high reactivity and high molecular
loading (see Chaps. 3 and 4), with the versatility and flexibility of soft lithography.
The transfer of amino end-functionalized poly(ethylene glycol) (PEG 500 -NH 2 ) from
oxidized poly(dimethyl siloxane) (PDMS) elastomer stamps to PNHSMA films was
shown by FTIR spectroscopy, X-ray photoelectron spectroscopy (XPS), fluorescence
microscopy, and ellipsometry measurements, which resulted in covalent coupling
and identical grafting densities as reported in Chap. 4 for coupling from solution.
The PEG-protected areas effectively inhibited the adsorption of fluoresceinamine,
bovine serum albumin (BSA), as well as 25mer DNA, while the unreacted NHS ester
groups retained their reactivity toward primary amino groups. Biomolecule microarrays were thus conveniently fabricated in a two-step procedure. The hybridization of
target DNA to immobilized probe DNA in micropatterns proved the concept of reactive μCP on activated polymer films for obtaining robust platforms for biomolecule
immobilization and screening.
© Shanghai Jiao Tong University Press 2021
C. Feng et al., Microfabrication of Stimuli-Responsive Polymers,
https://doi.org/10.1007/978-981-33-6869-9_6
109
Fabrication of Robust Biomolecular
Patterns by Reactive Microcontact
Printing on NHS Ester Containing
Polymer Films
In this chapter, the fabrication of robust biomolecule microarrays by reactive microcontact printing (μCP) on spin-coated thin films of poly(N-hydroxysuccinimidylmethacrylate) (PNHSMA) on oxidized silicon and glass is described. The approach
combines the advantages of activated polymer thin films as coupling layers, characterized by high reactivity and high molecular loading (see Chaps. 3 and 4), with the
versatility and flexibility of soft lithography. In this chapter, the fabrication of robust
biomolecule microarrays by reactive microcontact printing (μCP) on spin-coated thin
films of poly(N-hydroxysuccinimidyl-methacrylate) (PNHSMA) on oxidized silicon
and glass is described. The approach combines the advantages of activated polymer
thin films as coupling layers, characterized by high reactivity and high molecular
loading (see Chaps. 3 and 4), with the versatility and flexibility of soft lithography.
The transfer of amino end-functionalized poly(ethylene glycol) (PEG 500 -NH 2 ) from
oxidized poly(dimethyl siloxane) (PDMS) elastomer stamps to PNHSMA films was
shown by FTIR spectroscopy, X-ray photoelectron spectroscopy (XPS), fluorescence
microscopy, and ellipsometry measurements, which resulted in covalent coupling
and identical grafting densities as reported in Chap. 4 for coupling from solution.
The PEG-protected areas effectively inhibited the adsorption of fluoresceinamine,
bovine serum albumin (BSA), as well as 25mer DNA, while the unreacted NHS ester
groups retained their reactivity toward primary amino groups. Biomolecule microarrays were thus conveniently fabricated in a two-step procedure. The hybridization of
target DNA to immobilized probe DNA in micropatterns proved the concept of reactive μCP on activated polymer films for obtaining robust platforms for biomolecule
immobilization and screening.
© Shanghai Jiao Tong University Press 2021
C. Feng et al., Microfabrication of Stimuli-Responsive Polymers,
https://doi.org/10.1007/978-981-33-6869-9_6
109