106
5 Tailored Biointerfaces via Derivatization of Polystyrene-b-Poly …
Contact Angles Measurement: The contact angles were measured by a contact
angle microscope (Data Physis, OCA 15Plus) with Millipore water as the probe
liquid. Advancing and receding contact angles were measured at room temperature.
Infrared Spectroscopy: The FTIR spectra were obtained using a BIO-RAD model
FTS575C FTIR spectrometer equipped with a liquid nitrogen-cooled cryogenic
mercury cadmium telluride (MCT) detector. Spectral resolution was maintained at
4 cm
−1 with 1024 scans. Transmission mode FTIR spectra were used for the ultrathin
polymer films on silicon wafers.
Fluorescence Experiment. Fluorescence micrographs were taken using a Zeiss LSM
510 confocal fluorescence microscope with a LP 650 filter for isothiocyanates, Cy5labeled target DNA, and BSA conjugated with Alexa Fluor
® 594 with a 633 nm
He–Ne laser. For excitation, an Ar laser (30 mW) was used. A 650 nm long-pass
filter was selected for isothiocyanates, Cy5-labeled target DNA, and BSA conjugated
with Alexa Fluor
® 594, respectively.
References
1. Cheng X, Wang Y, Hanein Y, Bohringer KF (2004) Ratner BD (2004) Novel cell patterning
using microheater-controlled thermoresponsive plasma films. J Biomed Mater Res Part A
70A:159–168
2. Kasemo B (2002) Biological surface science. Surf Sci 500:656–677
3. Biran I, Walt DR (2002) Optical imaging fiber-based single live cell arrays: a high-density cell
array platform. Anal Chem 74:3046–3054
4. Ocvirk G, Salimi-Moosavi H, Szarka RJ, Arriaga EA, Andersson PE, Smith R, Dovichi
NJ, Harrison DJ (2004) beta-galactosidase assays of single-cell lysates on a microchip: a
complementary method for enzymatic analysis of single cells. Proc IEEE 92:115–125
5. Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE (1997) Geometric control of cell
life and death. Science 276:1425–1428
6. Mrksich M (2000) A surface chemistry approach to studying cell adhesion. Chem Soc Rev
29:267–273
7. Feng CL, Zhang Z, Förch R, Knoll W, Vancso GJ, Schönherr H (2005) Reactive thin polymer
films as platforms for the immobilization of biomolecules. Biomacromol 6:3243–3251
8. Gong P, Grainger DW (2004) Comparison of DNA immobilization efficiency on new and
regenerated commercial amine-reactive polymer microarray surface. Sur Sci 570:67–77
9. Schönherr H, Vancso GJ (1998) Surface properties of oxidized LDPE by scanning force
microscopy with chemically modified probes. J Polym Sci B Polym Phys 36:2483–2492
10. Bates FS, Fredrickson GH (1990) Block copolymer thermodynamics-theory and experiment.
Annu Rev Phys Chem 41:525–557
11. Tanaka K, Takahara A, Kajiyama T (1996) Film thickness dependence of the surface structure
of immiscible polystyrene/poly(methyl methacrylate) blends. Macromolecules 29:3232–3239
12. Groll J, Ameringer T, Spatz J, Möller M (2005) Ultrathin coatings from isocyanate-terminated
star PEG prepolymers: Layer formation and characterization. Langmuir 21:1991–1999
13. Aqua T, Naaman R, Daube SS (2003) Controlling the adsorption and reactivity of DNA on
gold. Langmuir 19:10573–10580
14. Kuželová K, Grebe´ nová D, Marinov I, Hrkal ZJ (2005) Fast apotosis and erythroid differentiation induced by imatinib mesylate in JURL-MK1 cells. Cellular Biochem 95:268–280
5 Tailored Biointerfaces via Derivatization of Polystyrene-b-Poly …
Contact Angles Measurement: The contact angles were measured by a contact
angle microscope (Data Physis, OCA 15Plus) with Millipore water as the probe
liquid. Advancing and receding contact angles were measured at room temperature.
Infrared Spectroscopy: The FTIR spectra were obtained using a BIO-RAD model
FTS575C FTIR spectrometer equipped with a liquid nitrogen-cooled cryogenic
mercury cadmium telluride (MCT) detector. Spectral resolution was maintained at
4 cm
−1 with 1024 scans. Transmission mode FTIR spectra were used for the ultrathin
polymer films on silicon wafers.
Fluorescence Experiment. Fluorescence micrographs were taken using a Zeiss LSM
510 confocal fluorescence microscope with a LP 650 filter for isothiocyanates, Cy5labeled target DNA, and BSA conjugated with Alexa Fluor
® 594 with a 633 nm
He–Ne laser. For excitation, an Ar laser (30 mW) was used. A 650 nm long-pass
filter was selected for isothiocyanates, Cy5-labeled target DNA, and BSA conjugated
with Alexa Fluor
® 594, respectively.
References
1. Cheng X, Wang Y, Hanein Y, Bohringer KF (2004) Ratner BD (2004) Novel cell patterning
using microheater-controlled thermoresponsive plasma films. J Biomed Mater Res Part A
70A:159–168
2. Kasemo B (2002) Biological surface science. Surf Sci 500:656–677
3. Biran I, Walt DR (2002) Optical imaging fiber-based single live cell arrays: a high-density cell
array platform. Anal Chem 74:3046–3054
4. Ocvirk G, Salimi-Moosavi H, Szarka RJ, Arriaga EA, Andersson PE, Smith R, Dovichi
NJ, Harrison DJ (2004) beta-galactosidase assays of single-cell lysates on a microchip: a
complementary method for enzymatic analysis of single cells. Proc IEEE 92:115–125
5. Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE (1997) Geometric control of cell
life and death. Science 276:1425–1428
6. Mrksich M (2000) A surface chemistry approach to studying cell adhesion. Chem Soc Rev
29:267–273
7. Feng CL, Zhang Z, Förch R, Knoll W, Vancso GJ, Schönherr H (2005) Reactive thin polymer
films as platforms for the immobilization of biomolecules. Biomacromol 6:3243–3251
8. Gong P, Grainger DW (2004) Comparison of DNA immobilization efficiency on new and
regenerated commercial amine-reactive polymer microarray surface. Sur Sci 570:67–77
9. Schönherr H, Vancso GJ (1998) Surface properties of oxidized LDPE by scanning force
microscopy with chemically modified probes. J Polym Sci B Polym Phys 36:2483–2492
10. Bates FS, Fredrickson GH (1990) Block copolymer thermodynamics-theory and experiment.
Annu Rev Phys Chem 41:525–557
11. Tanaka K, Takahara A, Kajiyama T (1996) Film thickness dependence of the surface structure
of immiscible polystyrene/poly(methyl methacrylate) blends. Macromolecules 29:3232–3239
12. Groll J, Ameringer T, Spatz J, Möller M (2005) Ultrathin coatings from isocyanate-terminated
star PEG prepolymers: Layer formation and characterization. Langmuir 21:1991–1999
13. Aqua T, Naaman R, Daube SS (2003) Controlling the adsorption and reactivity of DNA on
gold. Langmuir 19:10573–10580
14. Kuželová K, Grebe´ nová D, Marinov I, Hrkal ZJ (2005) Fast apotosis and erythroid differentiation induced by imatinib mesylate in JURL-MK1 cells. Cellular Biochem 95:268–280