46
2 Synthesis of In-Tether Chiral Center Peptides …
# cells
control
ER-1a
ER-1b
FITC
control
PDI-2a
PDI-2b
control
PDI-1a
PDI-1b
a)
b)
c)
d)
e)
Fig. 2.17 Cell permeability and in vitro serum stability of PDI peptides. (a) Fluorescent confocal
microscopy images of MCF-7 cells incubated with FITC-labeled peptides PDI-1a/1b (5 μM) at
37°C. (b) Fluorescent confocal microscopy images of MCF-7 cells incubated with FITC-labeled
peptides PDI-1b (5 μM) at 4°C or treated with NaN 3 + 2-Deoxyglucose for 1 h (DNA, blue (DAPI)
peptides, green (FITC). (c) FACS measurements of MCF-7 cells treated with ER-1a/1b, PDI-1a/1b,
and PDI-2a/2b (5 μM) at 37°C. For separated flowcytometry figures, see Figure S10. (d) In vitro
serum digestion assay of PDI-linear, PDI-1b, and PDI-2b peptides
could be useful for various applications. Studies on the chiral center effect and further
biological applications are currently underway and will be reported in due time.
2 Synthesis of In-Tether Chiral Center Peptides …
# cells
control
ER-1a
ER-1b
FITC
control
PDI-2a
PDI-2b
control
PDI-1a
PDI-1b
a)
b)
c)
d)
e)
Fig. 2.17 Cell permeability and in vitro serum stability of PDI peptides. (a) Fluorescent confocal
microscopy images of MCF-7 cells incubated with FITC-labeled peptides PDI-1a/1b (5 μM) at
37°C. (b) Fluorescent confocal microscopy images of MCF-7 cells incubated with FITC-labeled
peptides PDI-1b (5 μM) at 4°C or treated with NaN 3 + 2-Deoxyglucose for 1 h (DNA, blue (DAPI)
peptides, green (FITC). (c) FACS measurements of MCF-7 cells treated with ER-1a/1b, PDI-1a/1b,
and PDI-2a/2b (5 μM) at 37°C. For separated flowcytometry figures, see Figure S10. (d) In vitro
serum digestion assay of PDI-linear, PDI-1b, and PDI-2b peptides
could be useful for various applications. Studies on the chiral center effect and further
biological applications are currently underway and will be reported in due time.