92
3 In-Tether Chiral Center Induced Helical Peptide Modulators …
Table 3.2
MS characterization of peptide 1-12
Entry
Sequence
Calcu. MS Obsev. MS
1
FITC
βA L T F Q H Y
W A
Q
L T S
NH
2
1956.17
979.09
2
FITC
βA L T F C H Y
W S
5 (Me)
Q
L T S
NH
2
1999.29
1000.03
3
FITC
βA L T F C H Y
W S
5 (Ph)
Q
L T S
NH
2
2061.36
1030.2
4
FITC
βA L T F Q C Y
W A
S
5 (Me) L T S
NH
2
1933.23
967.63
5
FITC
βA L T F Q C Y
W A
S
5 (Ph)
L T S
NH
2
1995.3
999.02
6
FITC
βA L C F Q H S
5 (Me) W A
Q
L T S
NH
2
1934.22
967.96
7
FITC
βA
F C H Y
W S
5 (Me)
Q
L T S A A NH
2
1927.19
964.71
8
FITC
βA L T F C H Y
W S
5 (Me)
Q
L T S A A NH
2
2141.45
1071.75
9
FITC
βA L T F C H Y
W S
5 (Ph)
Q
L T S A A NH
2
2303.52
1152.5
10
FITC
βA L T F Q C Y
W A
S
5 (Me) L T S A A NH
2
2075.39
1038.99
11
FITC
βA L T F Q C Y
W A
S
5 (Ph)
L T S A A NH
2
2137.46
1070.13
12
FITC
βA L S
F C Q Y
W S
5 (Me)
Cba
L S P
NH
2
1970.29
986.35
MeR
Ac L T F C H Y
W S
5 (R-Me) Q
L T S
NH
2
789.8
790.1
PhR
Ac L T F C H Y
W S
5 (R-Ph)
Q
L T S
NH
2
821.3
821.4
PhR-Cy3
Cy3
βA L T F C H Y
W S
5 (R-Ph)
Q
L T S
NH
2
1056.1
1056.2
FITC-labeled peptides were used for FP and cellular uptake, Cy3 labeled peptides were used for in vivo imaging, acetyl peptides were used for all other
experiments