225
Marchesi E, Rota C, Fann YC, Chignell CF, Mason RP (1999) Photoreduction of the fluorescent
dye 2′-7′-dichlorofluorescein: a spin trapping and direct electron spin resonance study with
implications for oxidative stress measurements. Free Radic Biol Med 26:148–161
Meloni M, Nicolay JF (2003) Dynamic monitoring of glutathione redox status in UV-B irradiated
reconstituted epidermis: effect of antioxidant activity on skin homeostasis. Toxicol in Vitro
17:609–613
Mendes R, Koch B, Bachmatiuk A, El-Gendy A, Krupskaya Y, Springer A, Klingeler R, Schmidt
O, Buchner B, Sanchez S, Rummeli M (2014) Synthesis and toxicity characterization of carbon coated iron oxide nanoparticles with highly defined size distributions. Biochimica Et
Biophysica Acta-General Subjects 1840:160–169
Mishra D, Hubenak JR, Mathur AB (2013) Nanoparticle systems as tools to improve drug delivery
and therapeutic efficacy. J Biomed Mater Res A 101:3646–3660
Moghimi SM, Hunter AC, Murray JC (2005) Nanomedicine: current status and future prospects.
FASEB J 19:311–330
Monteiro-Riviere NA, Inman AO, Zhang LW (2009) Limitations and relative utility of screening
assays to assess engineered nanoparticle toxicity in a human cell line. Toxicol Appl Pharmacol
234:222–235
Myerson J, He L, Lanza G, Tollefsen D, Wickline S (2011) Thrombin-inhibiting perfluorocarbon
nanoparticles provide a novel strategy for the treatment and magnetic resonance imaging of
acute thrombosis. J Thromb Haemost 9:1292–1300
Neun BW, Dobrovolskaia MA (2011) Qualitative analysis of total complement activation by
nanoparticles. Methods Mol Biol 697:237–245
Ng CT, Yung LY, Swa HL, Poh RW, Gunaratne J, Bay BH (2015) Altered protein expression profile
associated with phenotypic changes in lung fibroblasts co-cultured with gold nanoparticletreated small airway epithelial cells. Biomaterials 39:31–38
Oberdörster E (2004) Manufactured nanomaterials (fullerenes, C60) induce oxidative stress in the
brain of juvenile largemouth bass. Environ Health Perspect 112:1058–1062
Omar NA, Praveena SM, Aris AZ, Hashim Z (2015) Health risk assessment using in vitro digestion model in assessing bioavailability of heavy metal in rice: a preliminary study. Food Chem
188:46–50
Ong KJ, MacCormack TJ, Clark RJ, Ede JD, Ortega VA, Felix LC, Dang MK, Ma G, Fenniri
H, Veinot JG, Goss GG (2014) Widespread nanoparticle-assay interference: implications for
nanotoxicity testing. PLoS One 9:e90650
Paglia G, Kliman M, Claude E, Geromanos S, Astarita G (2015) Applications of ion-mobility mass
spectrometry for lipid analysis. Anal Bioanal Chem 407:4995–5007
Patel P, Shah J (2017) Safety and toxicological considerations of nanomedicines: the future directions. Curr Clin Pharmacol
Peters M, Bonowitz P, Bufe A (2017) A bioassay for the determination of lipopolysaccharides and
lipoproteins. Methods Mol Biol 1600:143–150
Powell JJ, Faria N, Thomas-McKay E, Pele LC (2010) Origin and fate of dietary nanoparticles and
microparticles in the gastrointestinal tract. J Autoimmun 34:J226–J233
Rasmussen K, González M, Kearns P, Sintes JR, Rossi F, Sayre P (2016) Review of achievements of the OECD Working Party on Manufactured nanomaterials’ testing and assessment
programme. From exploratory testing to test guidelines. Regul Toxicol Pharmacol 74:147–160
Riss TL, Moravec RA, Niles AL (2011) Cytotoxicity testing: measuring viable cells, dead cells,
and detecting mechanism of cell death. Methods Mol Biol 740:103–114
Rowland M, Noe CR, Smith DA, Tucker GT, Crommelin DJ, Peck CC, Rocci ML Jr, Besancon L,
Shah VP (2012) Impact of the pharmaceutical sciences on health care: a reflection over the past
50 years. J Pharm Sci 101:4075–4099
Seitz F, Rosenfeldt RR, Schneider S, Schulz R, Bundschuh M (2014) Size-, surface- and crystalline structure composition-related effects of titanium dioxide nanoparticles during their aquatic
life cycle. Sci Total Environ 493:891–897
8 In Vitro Methodologies for Toxicological Assessment of Drug Delivery Nanocarriers
Marchesi E, Rota C, Fann YC, Chignell CF, Mason RP (1999) Photoreduction of the fluorescent
dye 2′-7′-dichlorofluorescein: a spin trapping and direct electron spin resonance study with
implications for oxidative stress measurements. Free Radic Biol Med 26:148–161
Meloni M, Nicolay JF (2003) Dynamic monitoring of glutathione redox status in UV-B irradiated
reconstituted epidermis: effect of antioxidant activity on skin homeostasis. Toxicol in Vitro
17:609–613
Mendes R, Koch B, Bachmatiuk A, El-Gendy A, Krupskaya Y, Springer A, Klingeler R, Schmidt
O, Buchner B, Sanchez S, Rummeli M (2014) Synthesis and toxicity characterization of carbon coated iron oxide nanoparticles with highly defined size distributions. Biochimica Et
Biophysica Acta-General Subjects 1840:160–169
Mishra D, Hubenak JR, Mathur AB (2013) Nanoparticle systems as tools to improve drug delivery
and therapeutic efficacy. J Biomed Mater Res A 101:3646–3660
Moghimi SM, Hunter AC, Murray JC (2005) Nanomedicine: current status and future prospects.
FASEB J 19:311–330
Monteiro-Riviere NA, Inman AO, Zhang LW (2009) Limitations and relative utility of screening
assays to assess engineered nanoparticle toxicity in a human cell line. Toxicol Appl Pharmacol
234:222–235
Myerson J, He L, Lanza G, Tollefsen D, Wickline S (2011) Thrombin-inhibiting perfluorocarbon
nanoparticles provide a novel strategy for the treatment and magnetic resonance imaging of
acute thrombosis. J Thromb Haemost 9:1292–1300
Neun BW, Dobrovolskaia MA (2011) Qualitative analysis of total complement activation by
nanoparticles. Methods Mol Biol 697:237–245
Ng CT, Yung LY, Swa HL, Poh RW, Gunaratne J, Bay BH (2015) Altered protein expression profile
associated with phenotypic changes in lung fibroblasts co-cultured with gold nanoparticletreated small airway epithelial cells. Biomaterials 39:31–38
Oberdörster E (2004) Manufactured nanomaterials (fullerenes, C60) induce oxidative stress in the
brain of juvenile largemouth bass. Environ Health Perspect 112:1058–1062
Omar NA, Praveena SM, Aris AZ, Hashim Z (2015) Health risk assessment using in vitro digestion model in assessing bioavailability of heavy metal in rice: a preliminary study. Food Chem
188:46–50
Ong KJ, MacCormack TJ, Clark RJ, Ede JD, Ortega VA, Felix LC, Dang MK, Ma G, Fenniri
H, Veinot JG, Goss GG (2014) Widespread nanoparticle-assay interference: implications for
nanotoxicity testing. PLoS One 9:e90650
Paglia G, Kliman M, Claude E, Geromanos S, Astarita G (2015) Applications of ion-mobility mass
spectrometry for lipid analysis. Anal Bioanal Chem 407:4995–5007
Patel P, Shah J (2017) Safety and toxicological considerations of nanomedicines: the future directions. Curr Clin Pharmacol
Peters M, Bonowitz P, Bufe A (2017) A bioassay for the determination of lipopolysaccharides and
lipoproteins. Methods Mol Biol 1600:143–150
Powell JJ, Faria N, Thomas-McKay E, Pele LC (2010) Origin and fate of dietary nanoparticles and
microparticles in the gastrointestinal tract. J Autoimmun 34:J226–J233
Rasmussen K, González M, Kearns P, Sintes JR, Rossi F, Sayre P (2016) Review of achievements of the OECD Working Party on Manufactured nanomaterials’ testing and assessment
programme. From exploratory testing to test guidelines. Regul Toxicol Pharmacol 74:147–160
Riss TL, Moravec RA, Niles AL (2011) Cytotoxicity testing: measuring viable cells, dead cells,
and detecting mechanism of cell death. Methods Mol Biol 740:103–114
Rowland M, Noe CR, Smith DA, Tucker GT, Crommelin DJ, Peck CC, Rocci ML Jr, Besancon L,
Shah VP (2012) Impact of the pharmaceutical sciences on health care: a reflection over the past
50 years. J Pharm Sci 101:4075–4099
Seitz F, Rosenfeldt RR, Schneider S, Schulz R, Bundschuh M (2014) Size-, surface- and crystalline structure composition-related effects of titanium dioxide nanoparticles during their aquatic
life cycle. Sci Total Environ 493:891–897
8 In Vitro Methodologies for Toxicological Assessment of Drug Delivery Nanocarriers