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13. McIntire WS, Wemmer DE, Chistoserdov A, Lidstrom ME (1991) A new cofactor in a prokaryotic enzyme: tryptophan tryptophylquinone as the redox prosthetic group in methylamine
dehydrogenase. Science 252:817–824
14. Ikeda T, Kobayashi D, Matsushita F, Sagara T, Niki K (1993) Bioelectrocatalysis at electrodes
coated with alcohol dehydrogenase, a quinohemoprotein with heme c serving as a built-in
mediator. J Electroanal Chem 361:221–228
15. Milton RD, Minteer SD (2017) Direct enzymatic bioelectrocatalysis: differentiating between
myth and reality. J R Soc Interface 14:20170253
16. Paddock RM, Bowden EF (1989) Electrocatalytic reduction of hydrogen peroxide via direct
electron transfer from pyrolytic graphite electrodes to irreversibly adsorbed cytochrome c
peroxidase. J Electroanal Chem 172:487–494
17. Xia HQ, Kitazumi Y, Shirai O, Kano K (2017) Direct electron transfer-type bioelectrocatalysis
of peroxidase at mesoporous carbon electrodes and its application for glucose determination
based on bienzyme system. Anal Sci 33:839–844
18. Blanford CF, Heath RS, Armstrong FA (2007) A stable electrode for high-potential, electrocatalytic O 2 reduction based on rational attachment of a blue copper oxidase to a graphite surface.
Chem Commun 1710–1712
19. So K, Kitazumi Y, Shirai O, Kano K (2016) Analysis of factors governing direct electron
transfer-type bioelectrocatalysis of bilirubin oxidase at modified electrodes. J Electroanal Chem
783:316–323
20. Murata K, Kajiya K, Nakamura N, Ohno H (2009) Direct electrochemistry of bilirubin oxidase
on three-dimensional gold nanoparticle electrodes and its application in a biofuel cell. Energy
Environ. Sci. 2:1280–1285
21. Funabashi H, Takeuchi S, Tsujimura S (2017) Hierarchical meso/macro-porous carbon fabricated from dual MgO templates for direct electron transfer enzymatic electrodes. Sci Rep
7:45147
22. Tsujimura S, Miura Y, Kano K (2008) CueO-immobilized porous carbon electrode exhibiting
improved performance of electrochemical reduction of dioxygen to water. Electrochim Acta
53:5716–5720
23. Wijma HJ, Jeuken LJC, Verbee MP, Armstrong FA, Canters GW (2007) Protein film voltammetry of copper-containing nitrite reductase reveals reversible inactivation. J Am Chem Soc
129:8557–8565
24. Pershad HR, Duff JLC, Heering HA, Duin EC, Albracht SPJ, Armstrong FA (1999) Catalytic
electron transport in chromatium vinosum [NiFe]-hydrogenase: application of voltammetry in
detecting redox-active centers and establishing that hydrogen oxidation is very fast even at
potentials close to the reversible H + /H 2 value. Biochemistry 38:8992–8999
25. Xia H-Q, So K, Kitazumi Y, Shirai O, Nishikawa K, Higuchi Y, Kano K (2016) Dual gasdiffusion membrane- and mediatorless dihydrogen/air-breathing biofuel cell operating at room
temperature. J Power Sources 335:105–112
26. So K, Hamamoto R, Takeuchi R, Kitazumi Y, Shirai O, Endo R, Nishihara H, Higuchi Y,
Kano K (2016) Bioelectrochemical analysis of thermodynamics of the catalytic cycle and
kinetics of the oxidative inactivation of oxygen-tolerant [NiFe]-hydrogenase. J Electroanal
Chem 766:152–161
27. Goldet G, Brandmayr C, Stripp ST, Happe T, Cavazza C, Fontecilla-Camps JC, Armstrong
FA (2009) Electrochemical kinetic investigations of the reactions of [FeFe]-hydrogenases
with carbon monoxide and oxygen: comparing the importance of gas tunnels and active-site
electronic/redox effects. J Am Chem Soc 131:14979–14989
28. Shiraiwa S, Kitazumi Y, Shirai O, Higuchi Y, Kano K, Unpublished
29. Sakai K, Kitazumi Y, Shirai O, Takagi K, Kano K (2017) Direct electron transfer-type
four-way bioelectrocatalysis of CO 2 /formate and NAD + /NADH redox couples by tungstencontaining formate dehydrogenase adsorbed on gold nanoparticle-embedded mesoporous
carbon electrodes modified with 4-mercaptopyridine. Electrochem Commun 84:75–79
30. Reda T, Plugge CM, Abram NJ, Hirst J (2008) Reversible interconversion of carbon dioxide
and formate by an electroactive enzyme. Proc Natl Acad Sci USA 105:10654–10658
75
13. McIntire WS, Wemmer DE, Chistoserdov A, Lidstrom ME (1991) A new cofactor in a prokaryotic enzyme: tryptophan tryptophylquinone as the redox prosthetic group in methylamine
dehydrogenase. Science 252:817–824
14. Ikeda T, Kobayashi D, Matsushita F, Sagara T, Niki K (1993) Bioelectrocatalysis at electrodes
coated with alcohol dehydrogenase, a quinohemoprotein with heme c serving as a built-in
mediator. J Electroanal Chem 361:221–228
15. Milton RD, Minteer SD (2017) Direct enzymatic bioelectrocatalysis: differentiating between
myth and reality. J R Soc Interface 14:20170253
16. Paddock RM, Bowden EF (1989) Electrocatalytic reduction of hydrogen peroxide via direct
electron transfer from pyrolytic graphite electrodes to irreversibly adsorbed cytochrome c
peroxidase. J Electroanal Chem 172:487–494
17. Xia HQ, Kitazumi Y, Shirai O, Kano K (2017) Direct electron transfer-type bioelectrocatalysis
of peroxidase at mesoporous carbon electrodes and its application for glucose determination
based on bienzyme system. Anal Sci 33:839–844
18. Blanford CF, Heath RS, Armstrong FA (2007) A stable electrode for high-potential, electrocatalytic O 2 reduction based on rational attachment of a blue copper oxidase to a graphite surface.
Chem Commun 1710–1712
19. So K, Kitazumi Y, Shirai O, Kano K (2016) Analysis of factors governing direct electron
transfer-type bioelectrocatalysis of bilirubin oxidase at modified electrodes. J Electroanal Chem
783:316–323
20. Murata K, Kajiya K, Nakamura N, Ohno H (2009) Direct electrochemistry of bilirubin oxidase
on three-dimensional gold nanoparticle electrodes and its application in a biofuel cell. Energy
Environ. Sci. 2:1280–1285
21. Funabashi H, Takeuchi S, Tsujimura S (2017) Hierarchical meso/macro-porous carbon fabricated from dual MgO templates for direct electron transfer enzymatic electrodes. Sci Rep
7:45147
22. Tsujimura S, Miura Y, Kano K (2008) CueO-immobilized porous carbon electrode exhibiting
improved performance of electrochemical reduction of dioxygen to water. Electrochim Acta
53:5716–5720
23. Wijma HJ, Jeuken LJC, Verbee MP, Armstrong FA, Canters GW (2007) Protein film voltammetry of copper-containing nitrite reductase reveals reversible inactivation. J Am Chem Soc
129:8557–8565
24. Pershad HR, Duff JLC, Heering HA, Duin EC, Albracht SPJ, Armstrong FA (1999) Catalytic
electron transport in chromatium vinosum [NiFe]-hydrogenase: application of voltammetry in
detecting redox-active centers and establishing that hydrogen oxidation is very fast even at
potentials close to the reversible H + /H 2 value. Biochemistry 38:8992–8999
25. Xia H-Q, So K, Kitazumi Y, Shirai O, Nishikawa K, Higuchi Y, Kano K (2016) Dual gasdiffusion membrane- and mediatorless dihydrogen/air-breathing biofuel cell operating at room
temperature. J Power Sources 335:105–112
26. So K, Hamamoto R, Takeuchi R, Kitazumi Y, Shirai O, Endo R, Nishihara H, Higuchi Y,
Kano K (2016) Bioelectrochemical analysis of thermodynamics of the catalytic cycle and
kinetics of the oxidative inactivation of oxygen-tolerant [NiFe]-hydrogenase. J Electroanal
Chem 766:152–161
27. Goldet G, Brandmayr C, Stripp ST, Happe T, Cavazza C, Fontecilla-Camps JC, Armstrong
FA (2009) Electrochemical kinetic investigations of the reactions of [FeFe]-hydrogenases
with carbon monoxide and oxygen: comparing the importance of gas tunnels and active-site
electronic/redox effects. J Am Chem Soc 131:14979–14989
28. Shiraiwa S, Kitazumi Y, Shirai O, Higuchi Y, Kano K, Unpublished
29. Sakai K, Kitazumi Y, Shirai O, Takagi K, Kano K (2017) Direct electron transfer-type
four-way bioelectrocatalysis of CO 2 /formate and NAD + /NADH redox couples by tungstencontaining formate dehydrogenase adsorbed on gold nanoparticle-embedded mesoporous
carbon electrodes modified with 4-mercaptopyridine. Electrochem Commun 84:75–79
30. Reda T, Plugge CM, Abram NJ, Hirst J (2008) Reversible interconversion of carbon dioxide
and formate by an electroactive enzyme. Proc Natl Acad Sci USA 105:10654–10658