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carbon electrodes modified with 4-mercaptopyridine. Electrochem Commun 84:75
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and electrode by immobilization of directly oriented enzyme molecules. J Fiber Sci Technol
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29. Kawai S, Goda-Tsutsumi M, Yakushi T, Kano K, Matsushita K (2013) Heterologous overexpression and characterization of a flavoprotein-cytochrome c complex fructose dehydrogenase
of Gluconobacter japonicus NBRC3260. Appl Environ Microbiol 79:1654–1660
30. Kawai S, Yakushi T, Matsushita K, Kitazumi Y, Shirai O, Kano K (2014) The electron transfer
pathway in direct electrochemical communication of fructose dehydrogenase with electrodes.
Electrochem Commun 38:28–31
31. Hibino Y, Kawai S, Kitazumi Y, Shirai O, Kano K (2017) Construction of a protein-engineered
variant of D-fructose dehydrogenase for direct electron transfer-type bioelectrocatalysis.
Electrochem Commun 77:112–115
32. Kaida Y, Hibino Y, Kitazumi Y, Shirai O, Kano K (2019) Ultimate downsizing of Dfructose dehydrogenase for improving the performance of direct electron transfer-type
bioelectrocatalysis. Electrochem Commun 98:101–105
33. Miura Y, Tsujimura S, Kurose S, Kamitaka Y, Kataoka K, Sakurai T, Kano K (2009) Direct
electrochemistry of CueO and its mutants at residues to and near type I Cu for oxygen-reducing
biocathode. Fuel Cells 9:70–78
34. Kamitaka Y, Tsujimura S, Kataoka K, Sakurai T, Ikeda T, Kano K (2007) Effects of axial
ligand mutation of the type I copper site in bilirubin oxidase on direct electron transfer-type
bioelectrocatalytic reduction of dioxygen. J Electroanal Chem 601:119–124
35. Zhang L, Cui H, Zou Z, Mirzaeigarakani T, Novoa-Henriquez C, Jooyeh B, Schwaneberg U
(2019) Directed evolution of a bacterial laccase (CueO) for enzymatic biofuel cells. Angew
Chem Int Ed 58:4562–4565
36. Hibino Y, Kawai S, Kitazumi Y, SHirai O, Kano K (2019) Protein-engineering improvement
of direct electron transfer-type bioelectrocatalytic properties of D-fructose dehydrogenase.
Electrochemistry 87:47–51
37. Kaida Y, Hibino Y, Kitazumi Y, Shirai O, Kano K (2020) Discussion on direct electron transfertype bioelectrocatalysis of downsized and axial-ligand exchanged variants of D-fructose
dehydrogenase. Electrochemistry 88:195–199
38. Sakai K, Xia H-Q, Kitazumi Y, Shirai O, Kano K (2018) Assembly of direct-electron-transfertype bioelectrodes with high performance. Electrochim Acta 271:305
39. Xia H-Q, Kitazumi Y, Shirai O, Kano K (2016) Enhanced direct electron transfer-type bioelectrocatalysis of bilirubin oxidase on negatively charged aromatic compound-modified carbon
electrode. J Electroanal Chem 763:104–109
40. So K, Kawai S, Hamano Y, Kitazumi Y, Shirai O, Hibi M, Ogawa J, Kano K (2014) Improvement of a direct electron transfer-type fructose/dioxygen biofuel cell with a substrate-modified
biocathode. Phys Chem Chem Phys 16:4823–4829
41. Xia H-Q, So K, Kitazumi Y, Shirai O, Nishikawa K, Higuchi Y, Kano K (2016) Dual gasdiffusion membrane- and mediatorless dihydrogen/air-breathing biofuel cell operating at room
temperature. J Power Sources 335:105–112
42. Xia H-Q, Hibino Y, Kitazumi Y, Shirai O, Kano K (2016) Interaction between d-fructose
dehydrogenase and methoxy-substituent-functionalized carbon surface to increase productive
orientations. Electrochim Acta 218:41–46
43. Sakai K, Kitazumi Y, Shirai O, Takagi K, Kano K (2017) Direct electron transfer-type
four-way bioelectrocatalysis of CO 2 /formate and NAD+/NADH redox couples by tungstencontaining formate dehydrogenase adsorbed on gold nanoparticle-embedded mesoporous
carbon electrodes modified with 4-mercaptopyridine. Electrochem Commun 84:75
44. Ferapontova E, Schmengler K, Borchers T, Ruzgas T, Gorton L (2002) Effect of cysteine
mutations on direct electron transfer of horseradish peroxidase on gold. Biosens Bioelectron
17:953–963
45. Takamura E, Suzuki H, Sakamoto H, Satomura T, Nakamura T, Sakuraba H, Ohshima T,
Suye S-I (2019) Improvement in electron transfer efficiency between multicopper oxidase
and electrode by immobilization of directly oriented enzyme molecules. J Fiber Sci Technol
75:47–51
46. Al-Lolage FA, Bartlett PN, Gounel Sb, Staigre P, Mano N (2019) Site-directed immobilization
of bilirubin oxidase for electrocatalytic oxygen reduction. ACS Catal 9:2068–2078