spermatozoa. Proc Natl Acad Sci U S A
98:13501–13506
37. Kaneko T, Nakagata N (2006) Improvement in
the long-term stability of freeze-dried mouse
spermatozoa by adding of a chelating agent.
Cryobiology 53:279–282
38. Nakai M, Kashiwazaki N, Takizawa A,
Maedomari N, Ozawa M, Noguchi J,
Kaneko H, Shino M, Kikuchi K (2007) Effects
of chelating agents during freeze-drying of
boar spermatozoa on DNA fragmentation and
on developmental ability in vitro and in vivo
after intracytoplasmic sperm head injection.
Zygote 15:15–24
39. Olaciregui M, Luno V, Marti JI, Aramayona J,
Gil L (2016) Freeze-dried stallion spermatozoa: evaluation of two chelating agents and
comparative analysis of three sperm DNA damage assays. Andrologia 48:900–906
40. Olaciregui M, Luno V, Gonzalez N,
Domingo P, de Blas I, Gil L (2017) Chelating
agents in combination with rosmarinic acid for
boar sperm freeze-drying. Reprod Biol
17:193–198
41. Domingo P, Olaciregui M, Gonzalez N, De
Blas I, Gil L (2018) Long-term preservation
of freeze-dried rabbit sperm by adding rosmarinic acid and different chelating agents. Cryobiology 81:174–177
42. Kaneko T, Whittingham DG, Yanagimachi R
(2003) Effect of pH value of freeze-drying
solution on the chromosome integrity and
developmental ability of mouse spermatozoa.
Biol Reprod 68:136–139
43. Kusakabe H, Tateno H (2017) Prevention of
high-temperature-induced chromosome damage in mouse spermatozoa freeze-dried using
Ca(2+) chelator-containing buffer alkalinized
with NaOH or KOH. Cryobiology 79:71–77
44. Olaciregui M, Luno V, Domingo P,
Gonzalez N, Gil L (2017) In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried
spermatozoa. Sci Rep 7:1096
45. Kaneko T, Whittingham DG, Overstreet JW,
Yanagimachi R (2003) Tolerance of the
mouse sperm nuclei to freeze-drying depends
on their disulfide status. Biol Reprod
69:1859–1862
46. Kawase Y, Araya H, Kamada N, Jishage K,
Suzuki H (2005) Possibility of long-term
preservation of freeze-dried mouse spermatozoa. Biol Reprod 72:568–573
47. Kaneko T, Nakagata N (2005) Relation
between storage temperature and fertilizing
ability of freeze-dried mouse spermatozoa.
Comp Med 55:140–144
48. Martins CF, Dode MN, Bao SN, Rumpf R
(2007) The use of the acridine orange test
and the TUNEL assay to assess the integrity
of freeze-dried bovine spermatozoa DNA.
Genet Mol Res 6:94–104
49. Oldenhof H, Zhang M, Narten K, Bigalk J,
Sydykov B, Wolkers WF, Sieme H (2017)
Freezing-induced uptake of disaccharides for
preservation of chromatin in freeze-dried stallion sperm during accelerated aging. Biol
Reprod 97:892–901
50. Ito D, Wakayama S, Kamada Y, Shibasaki I,
Kamimura S, Ooga M, Wakayama T (2019)
Effect of trehalose on the preservation of
freeze-dried mice spermatozoa at room temperature. J Reprod Dev. https://doi.org/10.
1262/jrd.2019-058
51. Ward MA, Kaneko T, Kusakabe H, Biggers JD,
Whittingham DG, Yanagimachi R (2003)
Long-term preservation of mouse spermatozoa
after freeze-drying and freezing without cryoprotection. Biol Reprod 69:2100–2108
52. Kawase Y, Tachibe T, Jishage K, Suzuki H
(2007) Transportation of freeze-dried mouse
spermatozoa under different preservation conditions. J Reprod Dev 53:1169–1174
53. Kaneko T, Serikawa T (2012) Long-term preservation of freeze-dried mouse spermatozoa.
Cryobiology 64:211–214
54. Kamada Y, Wakayama S, Shibasaki I, Ito D,
Kamimura S, Ooga M, Wakayama T (2018)
Assessing the tolerance to room temperature
and viability of freeze-dried mice spermatozoa
over long-term storage at room temperature
under vacuum. Sci Rep 8:10602
55. Hochi S, Watanabe K, Kato M, Hirabayashi M
(2008) Live rats resulting from injection of
oocytes with spermatozoa freeze-dried and
stored for one year. Mol Reprod Dev
75:890–894
56. Kaneko T, Kimura S, Nakagata N (2009)
Importance of primary culture conditions for
the development of rat ICSI embryos and
long-term preservation of freeze-dried sperm.
Cryobiology 58:293–297
730
Levent Keskintepe and Ali Eroglu
98:13501–13506
37. Kaneko T, Nakagata N (2006) Improvement in
the long-term stability of freeze-dried mouse
spermatozoa by adding of a chelating agent.
Cryobiology 53:279–282
38. Nakai M, Kashiwazaki N, Takizawa A,
Maedomari N, Ozawa M, Noguchi J,
Kaneko H, Shino M, Kikuchi K (2007) Effects
of chelating agents during freeze-drying of
boar spermatozoa on DNA fragmentation and
on developmental ability in vitro and in vivo
after intracytoplasmic sperm head injection.
Zygote 15:15–24
39. Olaciregui M, Luno V, Marti JI, Aramayona J,
Gil L (2016) Freeze-dried stallion spermatozoa: evaluation of two chelating agents and
comparative analysis of three sperm DNA damage assays. Andrologia 48:900–906
40. Olaciregui M, Luno V, Gonzalez N,
Domingo P, de Blas I, Gil L (2017) Chelating
agents in combination with rosmarinic acid for
boar sperm freeze-drying. Reprod Biol
17:193–198
41. Domingo P, Olaciregui M, Gonzalez N, De
Blas I, Gil L (2018) Long-term preservation
of freeze-dried rabbit sperm by adding rosmarinic acid and different chelating agents. Cryobiology 81:174–177
42. Kaneko T, Whittingham DG, Yanagimachi R
(2003) Effect of pH value of freeze-drying
solution on the chromosome integrity and
developmental ability of mouse spermatozoa.
Biol Reprod 68:136–139
43. Kusakabe H, Tateno H (2017) Prevention of
high-temperature-induced chromosome damage in mouse spermatozoa freeze-dried using
Ca(2+) chelator-containing buffer alkalinized
with NaOH or KOH. Cryobiology 79:71–77
44. Olaciregui M, Luno V, Domingo P,
Gonzalez N, Gil L (2017) In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried
spermatozoa. Sci Rep 7:1096
45. Kaneko T, Whittingham DG, Overstreet JW,
Yanagimachi R (2003) Tolerance of the
mouse sperm nuclei to freeze-drying depends
on their disulfide status. Biol Reprod
69:1859–1862
46. Kawase Y, Araya H, Kamada N, Jishage K,
Suzuki H (2005) Possibility of long-term
preservation of freeze-dried mouse spermatozoa. Biol Reprod 72:568–573
47. Kaneko T, Nakagata N (2005) Relation
between storage temperature and fertilizing
ability of freeze-dried mouse spermatozoa.
Comp Med 55:140–144
48. Martins CF, Dode MN, Bao SN, Rumpf R
(2007) The use of the acridine orange test
and the TUNEL assay to assess the integrity
of freeze-dried bovine spermatozoa DNA.
Genet Mol Res 6:94–104
49. Oldenhof H, Zhang M, Narten K, Bigalk J,
Sydykov B, Wolkers WF, Sieme H (2017)
Freezing-induced uptake of disaccharides for
preservation of chromatin in freeze-dried stallion sperm during accelerated aging. Biol
Reprod 97:892–901
50. Ito D, Wakayama S, Kamada Y, Shibasaki I,
Kamimura S, Ooga M, Wakayama T (2019)
Effect of trehalose on the preservation of
freeze-dried mice spermatozoa at room temperature. J Reprod Dev. https://doi.org/10.
1262/jrd.2019-058
51. Ward MA, Kaneko T, Kusakabe H, Biggers JD,
Whittingham DG, Yanagimachi R (2003)
Long-term preservation of mouse spermatozoa
after freeze-drying and freezing without cryoprotection. Biol Reprod 69:2100–2108
52. Kawase Y, Tachibe T, Jishage K, Suzuki H
(2007) Transportation of freeze-dried mouse
spermatozoa under different preservation conditions. J Reprod Dev 53:1169–1174
53. Kaneko T, Serikawa T (2012) Long-term preservation of freeze-dried mouse spermatozoa.
Cryobiology 64:211–214
54. Kamada Y, Wakayama S, Shibasaki I, Ito D,
Kamimura S, Ooga M, Wakayama T (2018)
Assessing the tolerance to room temperature
and viability of freeze-dried mice spermatozoa
over long-term storage at room temperature
under vacuum. Sci Rep 8:10602
55. Hochi S, Watanabe K, Kato M, Hirabayashi M
(2008) Live rats resulting from injection of
oocytes with spermatozoa freeze-dried and
stored for one year. Mol Reprod Dev
75:890–894
56. Kaneko T, Kimura S, Nakagata N (2009)
Importance of primary culture conditions for
the development of rat ICSI embryos and
long-term preservation of freeze-dried sperm.
Cryobiology 58:293–297
730
Levent Keskintepe and Ali Eroglu