6. Murashige and Skoog (MS) [20] basal medium, standardized
powder containing macro- and microelements (Duchefa Biochemie BV, Haarlem, the Netherlands) (see Note 1).
7. Murashige and Skoog [20] vitamin medium (MS with vitamin), standardized powder containing macro- and microelements and vitamins (Duchefa Biochemie BV, Haarlem, the
Netherlands).
8. Agar Agar Kobe I (SERVA Electrophoresis GmbH, Heidelberg, Germany).
9. For potato: MS medium with 2% sucrose, 1% agar at pH 5.8.
10. For mint, garlic and shallot: MS medium with vitamin, 3%
sucrose and 1% agar at pH 5.8.
2.2 Cryopreservation
2.2.1 Equipment
for Cryopreservation
1. Cryotank (MVE CryoSystem 6000; Cryotherm GmbH &
Co. KG, Kirchen/Siegen, Germany).
2. Boxes for cryovials.
3. Dewar vessel for liquid nitrogen.
4. Water bath.
5. Label printer.
2.2.2 Plant Preparation
and Cryopreservation
Treatment
1. Dissection binocular.
2. Sterile instruments: scalpel, forceps, pipette, counter, syringe,
and needle.
3. Cryovials 1.8 mL NUNC CryoTubes™ with internal thread
and starfoot (Fisher Scientific GmbH Schwerte, Germany).
4. Labels resistant to liquid nitrogen (Laser CRYO-BABIES,
Diversified Biotech, Dedham, USA).
5. Petri dishes (60 mm).
6. Round filter paper, grad 391, 45 mm.
7. Parafilm.
8. Ethanol 70% (v/v) for surface sterilization.
9. Liquid nitrogen.
2.2.3 Media
and Solutions
for Cryopreservation
1. Murashige and Skoog (MS) [20] basal medium (Duchefa Biochemie BV, Haarlem, the Netherlands) (see Note 1).
2. Murashige and Skoog [20] vitamin medium (MS with vitamin), standardized powder containing macro- and microelements and vitamins.
3. Preculture solution: MS medium with vitamin, 10% sucrose, at
pH 5.8 (liquid or solid).
4. Preculture medium for garlic inflorescences: MS medium with
vitamin, 10% sucrose, 2.0 mg/L 6-(y,y-Dimethylallylamino)
650
Angelika Senula and Manuela Nagel
powder containing macro- and microelements (Duchefa Biochemie BV, Haarlem, the Netherlands) (see Note 1).
7. Murashige and Skoog [20] vitamin medium (MS with vitamin), standardized powder containing macro- and microelements and vitamins (Duchefa Biochemie BV, Haarlem, the
Netherlands).
8. Agar Agar Kobe I (SERVA Electrophoresis GmbH, Heidelberg, Germany).
9. For potato: MS medium with 2% sucrose, 1% agar at pH 5.8.
10. For mint, garlic and shallot: MS medium with vitamin, 3%
sucrose and 1% agar at pH 5.8.
2.2 Cryopreservation
2.2.1 Equipment
for Cryopreservation
1. Cryotank (MVE CryoSystem 6000; Cryotherm GmbH &
Co. KG, Kirchen/Siegen, Germany).
2. Boxes for cryovials.
3. Dewar vessel for liquid nitrogen.
4. Water bath.
5. Label printer.
2.2.2 Plant Preparation
and Cryopreservation
Treatment
1. Dissection binocular.
2. Sterile instruments: scalpel, forceps, pipette, counter, syringe,
and needle.
3. Cryovials 1.8 mL NUNC CryoTubes™ with internal thread
and starfoot (Fisher Scientific GmbH Schwerte, Germany).
4. Labels resistant to liquid nitrogen (Laser CRYO-BABIES,
Diversified Biotech, Dedham, USA).
5. Petri dishes (60 mm).
6. Round filter paper, grad 391, 45 mm.
7. Parafilm.
8. Ethanol 70% (v/v) for surface sterilization.
9. Liquid nitrogen.
2.2.3 Media
and Solutions
for Cryopreservation
1. Murashige and Skoog (MS) [20] basal medium (Duchefa Biochemie BV, Haarlem, the Netherlands) (see Note 1).
2. Murashige and Skoog [20] vitamin medium (MS with vitamin), standardized powder containing macro- and microelements and vitamins.
3. Preculture solution: MS medium with vitamin, 10% sucrose, at
pH 5.8 (liquid or solid).
4. Preculture medium for garlic inflorescences: MS medium with
vitamin, 10% sucrose, 2.0 mg/L 6-(y,y-Dimethylallylamino)
650
Angelika Senula and Manuela Nagel