20. Ganeshan S, Rajasekharan PE, Shashikumar S,
Decruze W (2008) Cryopreservation of pollen.
In: Reed BM (ed) Plant cryopreservation: a
practical guide. Springer, New York, pp
443–464
21. Rajasekharan PE, Ravish BS, Kumar TV, Ganeshan S (2013) Pollen cryobanking for tropical
plant species. In: Normah MN, Chin HF, Reed
BM (eds) Conservation of tropical plant species. Springer, New York, pp 65–75
22. Philpott ME, Ballesteros D, Georgi LL,
Hebard FV, Culley TM, Pence VC (2015)
Seed production of American chestnut (Castanea dentata) using pollen stored 20 years in
liquid nitrogen. Poster presented at The American Chestnut Foundation 32nd annual
meeting. Pennsylvania State University, University Park, PA, 23–24 October, 2015
23. Ballesteros D, Nebot A, Pritchard HW (2019)
Cryobiotechnology for the long-term preservation of oak (Quercus sp.) genetic resources.
Acta Hortic 1234:37–46
24. Calonje M, Kay J, Griffith MP (2011) Propagation of cycad collections from seed: applied
reproductive biology for conservation. Sibbaldia 9:79–96
25. Nadarajan J, Benson EE, Xaba P, Harding K,
Lindstrom A, Donaldson J, Seal CE,
Kamoga D, Agoo EMG, Li N, King E, Pritchard HW (2018) Comparative biology of cycad
pollen, seed and tissue - a plant conservation
perspective. Bot Rev 84:295–314
26. Hoekstra FA (2002) Pollen and spores: desiccation tolerance in pollen and spores of lower
plants and fungi. In: Black M, Pritchard HW
(eds) Desiccation and survival in plants. Drying
without dying. CABI Publishing, Wallingford,
UK, pp 185–205
27. Nepi M, Franchi GG, Pacini E (2001) Pollen
hydration status at dispersal: cytophysiological
features
and
strategies.
Protoplasma
216:171–180
28. Franchi GG, Piotto B, Nepi M, Baskin CC,
Baskin JM, Pacini E (2011) Pollen and seed
desiccation tolerance in relation to degree of
developmental arrest, dispersal, and survival. J
Exp Bot 62:5267–5281
29. Alpert P, Oliver MJ (2002) Drying without
dying. In: Black M, Pritchard HW (eds) Desiccation and survival in plants. CABI Publishing,
Wallingford, UK, pp 3–43
30. Buitink J, Walters C, Hoekstra FA, Crane J
(1998) Storage behavior of Typha latifolia pollen at low water contents: interpretation on the
basis of water activity and glass concepts.
Physiol Plant 103:145–153
31. Nebot A, Pritchard HW, Ballesteros D (2018)
Desiccation tolerance and the hydration window for the cryopreservation of woody species’
pollen. Cryobiology 85:139
32. Franchi GG, Nepi M, Dafni A, Pacini E (2002)
Partially hydrated pollen: taxonomic distribution, ecological and evolutionary significance.
Plant Syst Evol 234:211–227
33. Barnabas B (1994) Preservation of maize pollen. In: Bajaj YPS (ed) Maize, Biotechnology in
agriculture and forestry, vol 25. Springer,
New York, pp 607–618
34. Luna V, Figueroa M, Baltazar M, Gomez L,
Townsend R, Schoper JB (2001) Maize pollen
longevity and distance isolation requirements
for effective pollen control. Crop Sci
41:1551–1557
35. Porter R (2019) Wild rice (Zizania L.) in
North America: genetic resources, conservation, and use. In: Greene S, Williams K,
Khoury C, Kantar M, Marek L (eds) North
American crop wild relatives, vol 2. Springer,
New York
36. Ballesteros D, Walters C (2007) Calorimetric
properties of water and triacylglycerols in fern
spores relating to storage at cryogenic temperatures. Cryobiology 55:1–9
37. Gold K, Hay F (2014) Technical Information
Sheet_09: Equilibrating seeds to specific moisture levels. Board of Trustees of the Royal
Botanic Gardens, Kew. http://brahmsonline.
kew.org/Content/Projects/msbp/resources/
Training/09-Seed-air-moisture-relations.pdf.
Accessed 28 July 2019
38. Ballesteros D, Narayan S, Varghese B, Sershen
(2018) Photo-oxidation modulates green fern
spore longevity during dry storage. Plant Cell
Tiss Org Cult 133:165–175
39. Vertucci CW, Roos EE, Crane J (1994) Theoretical basis of protocols for seed storage III.
Optimum moisture contents for pea seeds
stored at different temperatures. Ann Bot
74:531–540
40. Dafni A, Pacini E, Nepi M (2005) Pollen and
stigma biology. Pract Pollin Biol 3:111–121
41. Barnabas B, Rajki E (1976) Storage of maize
(Zea mays L.) pollen at À196
C in liquid nitrogen. Euphytica 25:747–752
42. Buitink J, Walters-Vertucci C, Hoekstra FA,
Leprince O (1996) Calorimetric properties of
dehydrating pollen (analysis of a desiccationtolerant and an intolerant species). Plant
Physiol 111:235–242
43. Yamaguchi E, Ishikawa M (2000) Cryopreservation of maize (Zea mays L.) pollen. In:
Engelmann F, Takagi H (eds) Cryopreservation
636
Anna Nebot et al.
Decruze W (2008) Cryopreservation of pollen.
In: Reed BM (ed) Plant cryopreservation: a
practical guide. Springer, New York, pp
443–464
21. Rajasekharan PE, Ravish BS, Kumar TV, Ganeshan S (2013) Pollen cryobanking for tropical
plant species. In: Normah MN, Chin HF, Reed
BM (eds) Conservation of tropical plant species. Springer, New York, pp 65–75
22. Philpott ME, Ballesteros D, Georgi LL,
Hebard FV, Culley TM, Pence VC (2015)
Seed production of American chestnut (Castanea dentata) using pollen stored 20 years in
liquid nitrogen. Poster presented at The American Chestnut Foundation 32nd annual
meeting. Pennsylvania State University, University Park, PA, 23–24 October, 2015
23. Ballesteros D, Nebot A, Pritchard HW (2019)
Cryobiotechnology for the long-term preservation of oak (Quercus sp.) genetic resources.
Acta Hortic 1234:37–46
24. Calonje M, Kay J, Griffith MP (2011) Propagation of cycad collections from seed: applied
reproductive biology for conservation. Sibbaldia 9:79–96
25. Nadarajan J, Benson EE, Xaba P, Harding K,
Lindstrom A, Donaldson J, Seal CE,
Kamoga D, Agoo EMG, Li N, King E, Pritchard HW (2018) Comparative biology of cycad
pollen, seed and tissue - a plant conservation
perspective. Bot Rev 84:295–314
26. Hoekstra FA (2002) Pollen and spores: desiccation tolerance in pollen and spores of lower
plants and fungi. In: Black M, Pritchard HW
(eds) Desiccation and survival in plants. Drying
without dying. CABI Publishing, Wallingford,
UK, pp 185–205
27. Nepi M, Franchi GG, Pacini E (2001) Pollen
hydration status at dispersal: cytophysiological
features
and
strategies.
Protoplasma
216:171–180
28. Franchi GG, Piotto B, Nepi M, Baskin CC,
Baskin JM, Pacini E (2011) Pollen and seed
desiccation tolerance in relation to degree of
developmental arrest, dispersal, and survival. J
Exp Bot 62:5267–5281
29. Alpert P, Oliver MJ (2002) Drying without
dying. In: Black M, Pritchard HW (eds) Desiccation and survival in plants. CABI Publishing,
Wallingford, UK, pp 3–43
30. Buitink J, Walters C, Hoekstra FA, Crane J
(1998) Storage behavior of Typha latifolia pollen at low water contents: interpretation on the
basis of water activity and glass concepts.
Physiol Plant 103:145–153
31. Nebot A, Pritchard HW, Ballesteros D (2018)
Desiccation tolerance and the hydration window for the cryopreservation of woody species’
pollen. Cryobiology 85:139
32. Franchi GG, Nepi M, Dafni A, Pacini E (2002)
Partially hydrated pollen: taxonomic distribution, ecological and evolutionary significance.
Plant Syst Evol 234:211–227
33. Barnabas B (1994) Preservation of maize pollen. In: Bajaj YPS (ed) Maize, Biotechnology in
agriculture and forestry, vol 25. Springer,
New York, pp 607–618
34. Luna V, Figueroa M, Baltazar M, Gomez L,
Townsend R, Schoper JB (2001) Maize pollen
longevity and distance isolation requirements
for effective pollen control. Crop Sci
41:1551–1557
35. Porter R (2019) Wild rice (Zizania L.) in
North America: genetic resources, conservation, and use. In: Greene S, Williams K,
Khoury C, Kantar M, Marek L (eds) North
American crop wild relatives, vol 2. Springer,
New York
36. Ballesteros D, Walters C (2007) Calorimetric
properties of water and triacylglycerols in fern
spores relating to storage at cryogenic temperatures. Cryobiology 55:1–9
37. Gold K, Hay F (2014) Technical Information
Sheet_09: Equilibrating seeds to specific moisture levels. Board of Trustees of the Royal
Botanic Gardens, Kew. http://brahmsonline.
kew.org/Content/Projects/msbp/resources/
Training/09-Seed-air-moisture-relations.pdf.
Accessed 28 July 2019
38. Ballesteros D, Narayan S, Varghese B, Sershen
(2018) Photo-oxidation modulates green fern
spore longevity during dry storage. Plant Cell
Tiss Org Cult 133:165–175
39. Vertucci CW, Roos EE, Crane J (1994) Theoretical basis of protocols for seed storage III.
Optimum moisture contents for pea seeds
stored at different temperatures. Ann Bot
74:531–540
40. Dafni A, Pacini E, Nepi M (2005) Pollen and
stigma biology. Pract Pollin Biol 3:111–121
41. Barnabas B, Rajki E (1976) Storage of maize
(Zea mays L.) pollen at À196
C in liquid nitrogen. Euphytica 25:747–752
42. Buitink J, Walters-Vertucci C, Hoekstra FA,
Leprince O (1996) Calorimetric properties of
dehydrating pollen (analysis of a desiccationtolerant and an intolerant species). Plant
Physiol 111:235–242
43. Yamaguchi E, Ishikawa M (2000) Cryopreservation of maize (Zea mays L.) pollen. In:
Engelmann F, Takagi H (eds) Cryopreservation
636
Anna Nebot et al.