6. In order to avoid nucleating ice inside the cell monolayer,
touch the vial with the cooled metal forceps just a little bit
above the solution.
7. Be aware that the warming may cause any liquid nitrogen
trapped inside the vial to expand and increase pressure in the
vial which may cause explosion or the glass to break.
Acknowledgments
Development of this protocol and preparation of this book chapter
were funded by the Canadian Institutes of Health Research (CIHR
MOP: 133684). N. Eskandari received a scholarship from the
University of Alberta. J.A.W. Elliott holds a Canada Research
Chair in Thermodynamics.
Conflict disclosure: A patent is being sought.
References
1. Feletou M (2011) Multiple functions of the
endothelial cells. The Endothelium. https://
www.ncbi.nlm.nih.gov/books/NBK57149
2. Eghrari AO, Riazuddin SA, Gottsch JD (2015)
Overview of the cornea: structure, function,
and development. Prog Mol Biol Transl Sci
134:7–23
3. Levis H, Kureshi A, Massie I, Morgan L,
Vernon A, Daniels J (2015) Tissue engineering
the cornea: The evolution of RAFT. J Funct
Biomater 6:50–65
4. Mimura T, Yamagami S, Amano S (2013) Corneal endothelial regeneration and tissue engineering. Prog Retin Eye Res 35:1–17
5. Proulx S, Brunette I (2012) Methods being
developed for preparation, delivery and transplantation of a tissue-engineered corneal endothelium. Exp Eye Res 95:68–75
6. Zhang Z, Niu G, Choi JS, Giegengack M,
Atala A, Soker S (2015) Bioengineered multilayered human corneas from discarded human
corneal tissue. Biomed Mater 10:1–9
7. Okumura N, Kinoshita S, Koizumi N (2014)
Cell-based approach for treatment of corneal
endothelial dysfunction. Cornea 33:37–41
8. Koizumi N, Okumura N, Kinoshita S (2012)
Development of new therapeutic modalities for
corneal endothelial disease focused on the proliferation of corneal endothelial cells using animal models. Exp Eye Res 95:60–67
9. Lehle K, Hoenicka M, Jacobs VR, Schmid FX,
Birnbaum DE (2005) Cryopreservation of
human endothelial cells for vascular tissue
engineering. Cryobiology 50:154–161
10. Polchow B, Kebbel K, Schmiedeknecht G,
Reichardt A, Henrich W, Hetzer R, Lueders C
(2012) Cryopreservation of human vascular
umbilical cord cells under good manufacturing
practice conditions for future cell banks. J
Transl Med 10:98–114
11. Marquez-Curtis LA, McGann LE, Elliott JAW
(2017) Expansion and cryopreservation of porcine and human corneal endothelial cells.
Cryobiology 77:1–13
12. Sultani AB, Marquez-Curtis LA, Elliott JAW,
McGann LE (2016) Improved cryopreservation of human umbilical vein endothelial cells:
a systematic approach. Sci Rep 6:1–14
13. McGann LE (1979) Optimal temperature
ranges for control of cooling rate. Cryobiology
16:211–216
14. Ross-Rodriguez LU, Elliott JAW, McGann LE
(2010) Investigating cryoinjury using simulations and experiments: 2. TF-1 cells during
graded freezing (interrupted slow cooling
without hold time). Cryobiology 61:46–51
15. McGann LE (1978) Differing actions of
penetrating and non-penetrating cryoprotective agents. Cryobiology 15:382–390
16. Stolzing A, Naaldijk Y, Fedorova V, Sethe S
(2012) Hydroxyethyl starch in cryopreservation—mechanisms, benefits and problems.
Transfus Apher Sci 46:137–147
590
Leah A. Marquez-Curtis et al.
touch the vial with the cooled metal forceps just a little bit
above the solution.
7. Be aware that the warming may cause any liquid nitrogen
trapped inside the vial to expand and increase pressure in the
vial which may cause explosion or the glass to break.
Acknowledgments
Development of this protocol and preparation of this book chapter
were funded by the Canadian Institutes of Health Research (CIHR
MOP: 133684). N. Eskandari received a scholarship from the
University of Alberta. J.A.W. Elliott holds a Canada Research
Chair in Thermodynamics.
Conflict disclosure: A patent is being sought.
References
1. Feletou M (2011) Multiple functions of the
endothelial cells. The Endothelium. https://
www.ncbi.nlm.nih.gov/books/NBK57149
2. Eghrari AO, Riazuddin SA, Gottsch JD (2015)
Overview of the cornea: structure, function,
and development. Prog Mol Biol Transl Sci
134:7–23
3. Levis H, Kureshi A, Massie I, Morgan L,
Vernon A, Daniels J (2015) Tissue engineering
the cornea: The evolution of RAFT. J Funct
Biomater 6:50–65
4. Mimura T, Yamagami S, Amano S (2013) Corneal endothelial regeneration and tissue engineering. Prog Retin Eye Res 35:1–17
5. Proulx S, Brunette I (2012) Methods being
developed for preparation, delivery and transplantation of a tissue-engineered corneal endothelium. Exp Eye Res 95:68–75
6. Zhang Z, Niu G, Choi JS, Giegengack M,
Atala A, Soker S (2015) Bioengineered multilayered human corneas from discarded human
corneal tissue. Biomed Mater 10:1–9
7. Okumura N, Kinoshita S, Koizumi N (2014)
Cell-based approach for treatment of corneal
endothelial dysfunction. Cornea 33:37–41
8. Koizumi N, Okumura N, Kinoshita S (2012)
Development of new therapeutic modalities for
corneal endothelial disease focused on the proliferation of corneal endothelial cells using animal models. Exp Eye Res 95:60–67
9. Lehle K, Hoenicka M, Jacobs VR, Schmid FX,
Birnbaum DE (2005) Cryopreservation of
human endothelial cells for vascular tissue
engineering. Cryobiology 50:154–161
10. Polchow B, Kebbel K, Schmiedeknecht G,
Reichardt A, Henrich W, Hetzer R, Lueders C
(2012) Cryopreservation of human vascular
umbilical cord cells under good manufacturing
practice conditions for future cell banks. J
Transl Med 10:98–114
11. Marquez-Curtis LA, McGann LE, Elliott JAW
(2017) Expansion and cryopreservation of porcine and human corneal endothelial cells.
Cryobiology 77:1–13
12. Sultani AB, Marquez-Curtis LA, Elliott JAW,
McGann LE (2016) Improved cryopreservation of human umbilical vein endothelial cells:
a systematic approach. Sci Rep 6:1–14
13. McGann LE (1979) Optimal temperature
ranges for control of cooling rate. Cryobiology
16:211–216
14. Ross-Rodriguez LU, Elliott JAW, McGann LE
(2010) Investigating cryoinjury using simulations and experiments: 2. TF-1 cells during
graded freezing (interrupted slow cooling
without hold time). Cryobiology 61:46–51
15. McGann LE (1978) Differing actions of
penetrating and non-penetrating cryoprotective agents. Cryobiology 15:382–390
16. Stolzing A, Naaldijk Y, Fedorova V, Sethe S
(2012) Hydroxyethyl starch in cryopreservation—mechanisms, benefits and problems.
Transfus Apher Sci 46:137–147
590
Leah A. Marquez-Curtis et al.