medium conditioned by teratocarcinoma stem
cells. Proc Natl Acad Sci U S A 78:7634–7638
5. Lo B, Parham L (2009) Ethical issues in stem
cell research. Endocr Rev 30:204–213
6. Takahashi K, Yamanaka S (2006) Induction of
pluripotent stem cells from mouse embryonic
and adult fibroblast cultures by defined factors.
Cell 126:663–676
7. Takahashi K, Tanabe K, Ohnuki M, Narita M,
Ichisaka T, Tomoda K, Yamanaka S (2007)
Induction of pluripotent stem cells from adult
human fibroblasts by defined factors. Cell
131:861–872
8. Yu J, Vodyanik MA, Smuga-Otto K,
Antosiewicz-Bourget J, Frane JL, Tian S,
Nie J, Jonsdottir GA, Ruotti V, Stewart R,
Slukvin II, Thomson JA (2007) Induced pluripotent stem cell lines derived from human
somatic cells. Science 318:1917–1920
9. Svendsen CN (2013) Back to the future: how
human induced pluripotent stem cells will
transform regenerative medicine. Hum Mol
Genet 22:R32–R38
10. Watanabe K, Ueno M, Kamiya D,
Nishiyama A, Matsumura M, Wataya T, Takahashi JB, Nishikawa S, Nishikawa S,
Muguruma K, Sasai Y (2007) A ROCK inhibitor permits survival of dissociated human
embryonic stem cells. Nat Biotechnol
25:681–686
11. Ha SY, Jee BC, Suh CS, Kim HS, Oh SK, Kim
SH, Moon SY (2005) Cryopreservation of
human embryonic stem cells without the use
of a programmable freezer. Hum Reprod
20:1779–1785
12. Martin-Ibanez R, Unger C, Stromberg A,
Baker D, Canals JM, Hovatta O (2008) Novel
cryopreservation method for dissociated
human embryonic stem cells in the presence
of a ROCK inhibitor. Hum Reprod
23:2744–2754
13. Li X, Meng G, Krawetz R, Liu S, Rancourt DE
(2008) The ROCK inhibitor Y-27632
enhances the survival rate of human embryonic
stem cells following cryopreservation. Stem
Cells Dev 17:1079–1085
14. Claassen DA, Desler MM, Rizzino A (2009)
ROCK inhibition enhances the recovery and
growth of cryopreserved human embryonic
stem cells and human induced pluripotent
stem cells. Mol Reprod Dev 76:722–732
15. Lopez M, Bollag RJ, Yu JC, Isales CM, Eroglu
A (2016) Chemically defined and xeno-free
cryopreservation of human adipose-derived
stem cells. PLoS One 11:e0152161
16. Szurek EA, Eroglu A (2011) Comparison and
avoidance of toxicity of penetrating cryoprotectants. PLoS One 6:e27604
17. Chen G, Gulbranson DR, Hou Z, Bolin JM,
Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee
GO, Antosiewicz-Bourget J, Teng JM, Thomson JA (2011) Chemically defined conditions
for human iPSC derivation and culture. Nat
Methods 8:424–429
18. Rajala K, Lindroos B, Hussein SM, Lappalainen RS, Pekkanen-Mattila M, Inzunza J,
Rozell B, Miettinen S, Narkilahti S, Kerkela E,
Aalto-Setala K, Otonkoski T, Suuronen R,
Hovatta O, Skottman H (2010) A defined
and xeno-free culture method enabling the
establishment of clinical-grade human embryonic, induced pluripotent and adipose stem
cells. PLoS One 5:e10246
19. Yasuda SY, Ikeda T, Shahsavarani H,
Yoshida N, Nayer B, Hino M, Vartak-SharmaN, Suemori H, Hasegawa K (2018) Chemically
defined and growth-factor-free culture system
for the expansion and derivation of human
pluripotent stem cells. Nat Biomed Eng
2:173–182
Chemically Defined Cryopreservation of hiPSCs
579
cells. Proc Natl Acad Sci U S A 78:7634–7638
5. Lo B, Parham L (2009) Ethical issues in stem
cell research. Endocr Rev 30:204–213
6. Takahashi K, Yamanaka S (2006) Induction of
pluripotent stem cells from mouse embryonic
and adult fibroblast cultures by defined factors.
Cell 126:663–676
7. Takahashi K, Tanabe K, Ohnuki M, Narita M,
Ichisaka T, Tomoda K, Yamanaka S (2007)
Induction of pluripotent stem cells from adult
human fibroblasts by defined factors. Cell
131:861–872
8. Yu J, Vodyanik MA, Smuga-Otto K,
Antosiewicz-Bourget J, Frane JL, Tian S,
Nie J, Jonsdottir GA, Ruotti V, Stewart R,
Slukvin II, Thomson JA (2007) Induced pluripotent stem cell lines derived from human
somatic cells. Science 318:1917–1920
9. Svendsen CN (2013) Back to the future: how
human induced pluripotent stem cells will
transform regenerative medicine. Hum Mol
Genet 22:R32–R38
10. Watanabe K, Ueno M, Kamiya D,
Nishiyama A, Matsumura M, Wataya T, Takahashi JB, Nishikawa S, Nishikawa S,
Muguruma K, Sasai Y (2007) A ROCK inhibitor permits survival of dissociated human
embryonic stem cells. Nat Biotechnol
25:681–686
11. Ha SY, Jee BC, Suh CS, Kim HS, Oh SK, Kim
SH, Moon SY (2005) Cryopreservation of
human embryonic stem cells without the use
of a programmable freezer. Hum Reprod
20:1779–1785
12. Martin-Ibanez R, Unger C, Stromberg A,
Baker D, Canals JM, Hovatta O (2008) Novel
cryopreservation method for dissociated
human embryonic stem cells in the presence
of a ROCK inhibitor. Hum Reprod
23:2744–2754
13. Li X, Meng G, Krawetz R, Liu S, Rancourt DE
(2008) The ROCK inhibitor Y-27632
enhances the survival rate of human embryonic
stem cells following cryopreservation. Stem
Cells Dev 17:1079–1085
14. Claassen DA, Desler MM, Rizzino A (2009)
ROCK inhibition enhances the recovery and
growth of cryopreserved human embryonic
stem cells and human induced pluripotent
stem cells. Mol Reprod Dev 76:722–732
15. Lopez M, Bollag RJ, Yu JC, Isales CM, Eroglu
A (2016) Chemically defined and xeno-free
cryopreservation of human adipose-derived
stem cells. PLoS One 11:e0152161
16. Szurek EA, Eroglu A (2011) Comparison and
avoidance of toxicity of penetrating cryoprotectants. PLoS One 6:e27604
17. Chen G, Gulbranson DR, Hou Z, Bolin JM,
Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee
GO, Antosiewicz-Bourget J, Teng JM, Thomson JA (2011) Chemically defined conditions
for human iPSC derivation and culture. Nat
Methods 8:424–429
18. Rajala K, Lindroos B, Hussein SM, Lappalainen RS, Pekkanen-Mattila M, Inzunza J,
Rozell B, Miettinen S, Narkilahti S, Kerkela E,
Aalto-Setala K, Otonkoski T, Suuronen R,
Hovatta O, Skottman H (2010) A defined
and xeno-free culture method enabling the
establishment of clinical-grade human embryonic, induced pluripotent and adipose stem
cells. PLoS One 5:e10246
19. Yasuda SY, Ikeda T, Shahsavarani H,
Yoshida N, Nayer B, Hino M, Vartak-SharmaN, Suemori H, Hasegawa K (2018) Chemically
defined and growth-factor-free culture system
for the expansion and derivation of human
pluripotent stem cells. Nat Biomed Eng
2:173–182
Chemically Defined Cryopreservation of hiPSCs
579