11. Puissant N, Barreau C, Bourin P, Clavel C,
Corre J, Bousquet C, Taureau C, Cousin B,
Abbal M, Laharrague P, Penicaud L,
Casteilla L, Blancher A (2005) Immunomodulatory effect of human adipose tissue-derived
adult stem cells: comparison with bone marrow
mesenchymal stem cells. Br J Haematol
129:118–129
12. Niemeyer P, Vohrer J, Schmal H, Kasten P,
Fellenberg J, Suedkamp NP, Mehlhorn AT
(2008) Survival of human mesenchymal stromal cells from bone marrow and adipose tissue
after xenogenic transplantation in immunocompetent mice. Cytotherapy 10:784–795
13. Gonzalez-Rey E, Gonzalez MA, Varela N,
O’Valle F, Hernandez-Cortes P, Rico L,
Buscher D, Delgado M (2010) Human
adipose-derived mesenchymal stem cells reduce
inflammatory and T cell responses and induce
regulatory T cells in vitro in rheumatoid arthritis. Ann Rheum Dis 69:241–248
14. Garcia-Olmo
D,
Garcia-Arranz
M,
Herreros D, Pascual I, Peiro C, RodriguezMontes JA (2005) A phase I clinical trial of
the treatment of Crohn’s fistula by adipose
mesenchymal stem cell transplantation. Dis
Colon Rectum 48:1416–1423
15. Nambu M, Ishihara M, Nakamura S,
Mizuno H, Yanagibayashi S, Kanatani Y,
Hattori H, Takase B, Ishizuka T, Kishimoto S,
Amano Y, Yamamoto N, Azuma R, Kiyosawa T
(2007) Enhanced healing of mitomycin
C-treated wounds in rats using inbred adipose
tissue-derived stromal cells within an atelocollagen matrix. Wound Repair Regen
15:505–510
16. Cowan CM, Shi YY, Aalami OO, Chou YF,
Mari C, Thomas R, Quarto N, Contag CH,
Wu B, Longaker MT (2004) Adipose-derived
adult stromal cells heal critical-size mouse calvarial defects. Nature Biotechnol 22:560–567
17. Cui L, Liu B, Liu G, Zhang W, Cen L, Sun J,
Yin S, Liu W, Cao Y (2007) Repair of cranial
bone defects with adipose derived stem cells
and coral scaffold in a canine model. Biomaterials 28:5477–5486
18. Mizuno H, Tobita M, Uysal AC (2012) Concise review: Adipose-derived stem cells as a
novel tool for future regenerative medicine.
Stem Cells 30:804–810
19. Lopez M, Bollag RJ, Yu JC, Isales CM, Eroglu
A (2016) Chemically defined and xeno-free
cryopreservation of human adipose-derived
stem cells. PLoS One 11:e0152161
20. Szurek EA, Eroglu A (2011) Comparison and
avoidance of toxicity of penetrating cryoprotectants. PLoS One 6:e2760
21. Hubel A (2018) Preservation of cells: a practical manual. John Wiley & Sons, Inc., Hoboken, NJ, pp 131–139
22. Grisendi G, Anneren C, Cafarelli L, Sternieri R,
Veronesi E, Cervo GL, Luminari S, Maur M,
Frassoldati A, Palazzi G, Otsuru S, Bambi F,
Paolucci P, Pierfranco C, Horwitz E, Dominici
M (2010) GMP-manufactured density gradient media for optimized mesenchymal stromal/stem cell isolation and expansion.
Cytotherapy 12:466–477
23. Brooke G, Rossetti T, Pelekanos R, Ilic N,
Murray P, Hancock S, Antonenas V,
Huang G, Gottlieb D, Bradstock K, Atkinson
K (2009) Manufacturing of human placentaderived mesenchymal stem cells for clinical
trials. Br J Haem 144:571–579
Chemically Defined Cryopreservation of Human ASCs
567
Corre J, Bousquet C, Taureau C, Cousin B,
Abbal M, Laharrague P, Penicaud L,
Casteilla L, Blancher A (2005) Immunomodulatory effect of human adipose tissue-derived
adult stem cells: comparison with bone marrow
mesenchymal stem cells. Br J Haematol
129:118–129
12. Niemeyer P, Vohrer J, Schmal H, Kasten P,
Fellenberg J, Suedkamp NP, Mehlhorn AT
(2008) Survival of human mesenchymal stromal cells from bone marrow and adipose tissue
after xenogenic transplantation in immunocompetent mice. Cytotherapy 10:784–795
13. Gonzalez-Rey E, Gonzalez MA, Varela N,
O’Valle F, Hernandez-Cortes P, Rico L,
Buscher D, Delgado M (2010) Human
adipose-derived mesenchymal stem cells reduce
inflammatory and T cell responses and induce
regulatory T cells in vitro in rheumatoid arthritis. Ann Rheum Dis 69:241–248
14. Garcia-Olmo
D,
Garcia-Arranz
M,
Herreros D, Pascual I, Peiro C, RodriguezMontes JA (2005) A phase I clinical trial of
the treatment of Crohn’s fistula by adipose
mesenchymal stem cell transplantation. Dis
Colon Rectum 48:1416–1423
15. Nambu M, Ishihara M, Nakamura S,
Mizuno H, Yanagibayashi S, Kanatani Y,
Hattori H, Takase B, Ishizuka T, Kishimoto S,
Amano Y, Yamamoto N, Azuma R, Kiyosawa T
(2007) Enhanced healing of mitomycin
C-treated wounds in rats using inbred adipose
tissue-derived stromal cells within an atelocollagen matrix. Wound Repair Regen
15:505–510
16. Cowan CM, Shi YY, Aalami OO, Chou YF,
Mari C, Thomas R, Quarto N, Contag CH,
Wu B, Longaker MT (2004) Adipose-derived
adult stromal cells heal critical-size mouse calvarial defects. Nature Biotechnol 22:560–567
17. Cui L, Liu B, Liu G, Zhang W, Cen L, Sun J,
Yin S, Liu W, Cao Y (2007) Repair of cranial
bone defects with adipose derived stem cells
and coral scaffold in a canine model. Biomaterials 28:5477–5486
18. Mizuno H, Tobita M, Uysal AC (2012) Concise review: Adipose-derived stem cells as a
novel tool for future regenerative medicine.
Stem Cells 30:804–810
19. Lopez M, Bollag RJ, Yu JC, Isales CM, Eroglu
A (2016) Chemically defined and xeno-free
cryopreservation of human adipose-derived
stem cells. PLoS One 11:e0152161
20. Szurek EA, Eroglu A (2011) Comparison and
avoidance of toxicity of penetrating cryoprotectants. PLoS One 6:e2760
21. Hubel A (2018) Preservation of cells: a practical manual. John Wiley & Sons, Inc., Hoboken, NJ, pp 131–139
22. Grisendi G, Anneren C, Cafarelli L, Sternieri R,
Veronesi E, Cervo GL, Luminari S, Maur M,
Frassoldati A, Palazzi G, Otsuru S, Bambi F,
Paolucci P, Pierfranco C, Horwitz E, Dominici
M (2010) GMP-manufactured density gradient media for optimized mesenchymal stromal/stem cell isolation and expansion.
Cytotherapy 12:466–477
23. Brooke G, Rossetti T, Pelekanos R, Ilic N,
Murray P, Hancock S, Antonenas V,
Huang G, Gottlieb D, Bradstock K, Atkinson
K (2009) Manufacturing of human placentaderived mesenchymal stem cells for clinical
trials. Br J Haem 144:571–579
Chemically Defined Cryopreservation of Human ASCs
567