Acknowledgments
This work was supported by the Science and Technology Research
Partnership for Sustainable Development (SATREPS) of Japan
Science and Technology Agency (JST)/Japan International Cooperation Agency (JICA).
References
1. Nagashima H, Kashiwazaki N, Ashman RJ,
Grupen CG, Seamark RF, Nottle MB (1994)
Removal of cytoplasmic lipid enhances the tolerance of porcine embryos to chilling. Biol
Reprod 51:618–622
2. Didion BA, Pomp D, Martin MJ, Homanics
GE, Markert CL (1990) Observations on the
cooling and cryopreservation of pig oocytes at
the germinal vesicle stage. J Anim Sci
68:2803–2810
3. Zhou GB, Li N (2009) Cryopreservation of
porcine oocytes: recent advances. Mol Hum
Reprod 15:279–285
4. Egerszegi I, Somfai T, Nakai M, Tanihara F,
Noguchi J, Kaneko H, Nagai T, Ra ´tky J, Kikuchi K (2013) Comparison of cytoskeletal integrity,
fertilization
and
developmental
competence of oocytes vitrified before or after
in vitro maturation in a porcine model. Cryobiology 67:287–292
5. Papis K, Shimizu M, Izaike Y (2000) Factors
affecting the survivability of bovine oocytes
vitrified
in
droplets.
Theriogenology
54:651–658
6. Somfai T, Yoshioka K, Tanihara F, Kaneko H,
Noguchi J, Kashiwazaki N, Nagai T, Kikuchi K
(2014) Generation of live piglets from cryopreserved oocytes for the first time using a defined
system for in vitro embryo production. PLoS
One 9:e97731
7. Somfai T, Ozawa M, Noguchi J, Kaneko H,
Nakai M, Maedomari N, Ito J, Kashiwazaki N,
Nagai T, Kikuchi K (2009) Live piglets derived
from in vitro-produced zygotes vitrified at the
pronuclear stage. Biol Reprod 80:42–49
8. Grupen CG, Somfai T, Kikuchi K (2016)
Effect of l-carnitine treatment during oocyte
maturation on the post-thaw development of
porcine embryos vitrified at the pronuclear
stage. Reprod Fert Dev 28:153–154 (abstract)
9. Nguyen VK, Vu HTT, Nguyen HT, Quan HX,
Pham LD, Kikuchi K, Nguyen ST, Somfai T
(2018) Comparison of the microdrop and
minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes
and blastocysts after equilibration in low concentrations of cryoprotectant agents. J Reprod
Dev 64:457–462
10. Somfai T, Nakai M, Tanihara F, Noguchi J,
Kaneko H, Kashiwazaki N, Egerszegi I,
Nagai T, Kikuchi K (2013) Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes. J Reprod
Dev 59:378–384
11. Somfai T, Men NT, Noguchi J, Kaneko H,
Kashiwazaki N, Kikuchi K (2015) Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed porcine
oocytes: comparison of sugars, combinations of
permeating cryoprotectants and equilibration
regimens. J Reprod Dev 61:571–579
12. Appeltant R, Somfai T, Kikuchi K (2018) Faster, cheaper, defined and efficient vitrification
for immature porcine oocytes through modification of exposure time, macromolecule source
and temperature. Cryobiology 85:87–94
13. Petters R, Wells K (1993) Culture of pig
embryos. J Reprod Fert Suppl 48:61–73
14. Somfai T, Hirao Y (2017) Synchronization of
in vitro maturation in porcine oocytes. In: Banfalvi G (ed) Cell cycle synchronization, Methods in molecular biology, vol 1524. Humana
Press, New York, NY, pp 255–264
15. Yoshioka K, Suzuki C, Onishi A (2008)
Defined system for in vitro production of porcine embryos using a single basic medium. J
Reprod Dev 54:208–213
16. Mohr LR, Trounson AO (1980) The use of
fluorescein diacetate to assess embryo viability
in the mouse. J Reprod Fert 58:189–196
17. Shi WQ, Zhu SE, Zhang D, Wang WH, Tang
GL, Hou YP, Tian SJ (2006) Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes.
Reproduction 131:795–804
468
Tamas Somfai and Kazuhiro Kikuchi
This work was supported by the Science and Technology Research
Partnership for Sustainable Development (SATREPS) of Japan
Science and Technology Agency (JST)/Japan International Cooperation Agency (JICA).
References
1. Nagashima H, Kashiwazaki N, Ashman RJ,
Grupen CG, Seamark RF, Nottle MB (1994)
Removal of cytoplasmic lipid enhances the tolerance of porcine embryos to chilling. Biol
Reprod 51:618–622
2. Didion BA, Pomp D, Martin MJ, Homanics
GE, Markert CL (1990) Observations on the
cooling and cryopreservation of pig oocytes at
the germinal vesicle stage. J Anim Sci
68:2803–2810
3. Zhou GB, Li N (2009) Cryopreservation of
porcine oocytes: recent advances. Mol Hum
Reprod 15:279–285
4. Egerszegi I, Somfai T, Nakai M, Tanihara F,
Noguchi J, Kaneko H, Nagai T, Ra ´tky J, Kikuchi K (2013) Comparison of cytoskeletal integrity,
fertilization
and
developmental
competence of oocytes vitrified before or after
in vitro maturation in a porcine model. Cryobiology 67:287–292
5. Papis K, Shimizu M, Izaike Y (2000) Factors
affecting the survivability of bovine oocytes
vitrified
in
droplets.
Theriogenology
54:651–658
6. Somfai T, Yoshioka K, Tanihara F, Kaneko H,
Noguchi J, Kashiwazaki N, Nagai T, Kikuchi K
(2014) Generation of live piglets from cryopreserved oocytes for the first time using a defined
system for in vitro embryo production. PLoS
One 9:e97731
7. Somfai T, Ozawa M, Noguchi J, Kaneko H,
Nakai M, Maedomari N, Ito J, Kashiwazaki N,
Nagai T, Kikuchi K (2009) Live piglets derived
from in vitro-produced zygotes vitrified at the
pronuclear stage. Biol Reprod 80:42–49
8. Grupen CG, Somfai T, Kikuchi K (2016)
Effect of l-carnitine treatment during oocyte
maturation on the post-thaw development of
porcine embryos vitrified at the pronuclear
stage. Reprod Fert Dev 28:153–154 (abstract)
9. Nguyen VK, Vu HTT, Nguyen HT, Quan HX,
Pham LD, Kikuchi K, Nguyen ST, Somfai T
(2018) Comparison of the microdrop and
minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes
and blastocysts after equilibration in low concentrations of cryoprotectant agents. J Reprod
Dev 64:457–462
10. Somfai T, Nakai M, Tanihara F, Noguchi J,
Kaneko H, Kashiwazaki N, Egerszegi I,
Nagai T, Kikuchi K (2013) Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes. J Reprod
Dev 59:378–384
11. Somfai T, Men NT, Noguchi J, Kaneko H,
Kashiwazaki N, Kikuchi K (2015) Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed porcine
oocytes: comparison of sugars, combinations of
permeating cryoprotectants and equilibration
regimens. J Reprod Dev 61:571–579
12. Appeltant R, Somfai T, Kikuchi K (2018) Faster, cheaper, defined and efficient vitrification
for immature porcine oocytes through modification of exposure time, macromolecule source
and temperature. Cryobiology 85:87–94
13. Petters R, Wells K (1993) Culture of pig
embryos. J Reprod Fert Suppl 48:61–73
14. Somfai T, Hirao Y (2017) Synchronization of
in vitro maturation in porcine oocytes. In: Banfalvi G (ed) Cell cycle synchronization, Methods in molecular biology, vol 1524. Humana
Press, New York, NY, pp 255–264
15. Yoshioka K, Suzuki C, Onishi A (2008)
Defined system for in vitro production of porcine embryos using a single basic medium. J
Reprod Dev 54:208–213
16. Mohr LR, Trounson AO (1980) The use of
fluorescein diacetate to assess embryo viability
in the mouse. J Reprod Fert 58:189–196
17. Shi WQ, Zhu SE, Zhang D, Wang WH, Tang
GL, Hou YP, Tian SJ (2006) Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes.
Reproduction 131:795–804
468
Tamas Somfai and Kazuhiro Kikuchi