2. A special glass graduated funnel-shaped tube may be used for
sperm collection or just a standard graduated tube. Preferably,
the semen extender (ASG poultry extender (ASG-PE)) and the
collection tubes are placed in a 25
C incubator in the barn.
This is only necessary at low barn temperatures.
3. A cock is fixed by one person between the arm and body with
the tail pointing forward, holding the legs between the fingers,
while a second person collects the semen. This person strokes a
few times with his hand over the back of the cock from
shoulders toward the tail, exerting some pressure. This will
cause the tail to be raised. The behavior of the animal indicates
its readiness to ejaculate, and the semen collector will hold the
prewarmed tube underneath the cloaca while pinching the
cloaca with two or three fingers. Try to collect the semen
without feces or other contaminations. It is advised to withdraw feed prior to planned semen collection.
Table 2
Medium composition of “Solution 1” [59]
g/100 mL
mmol/L
Sodium-L-glutamate.H 2 O
1.92
102.6
Magnesium acetate.4H 2 O
0.08
3.73
Fructose
0.8
44.4
Potassium acetate
0.5
50.9
Polyvinylpyrrolidone
0.3
0.08
Pure water (e.g., Milli-Q)
To 100 mL
pH ¼ 6.9
Table 3
Preparation of “three times concentrated” CPA medium. This medium (cooled) can be added as one
volume to two volumes of prediluted cooled semen to obtain the desired final CPA concentrations
(see Notes 1 and 2)
3Â concentrated Final concentration
g/100 mL Mol/L g/100 mL Mol/L
Weigh all chemicals for the diluent as given in Tables 1 or 2 15.7
1.8
5.2
0.6
Dissolve in pure water (e.g., Milli-Q)
Also add 15.68 g DMA
Or
33
3.6
11
1.2
Also add 33 g glycerol
Fill with pure water to 100 mL
390
Henri Woelders