7. Baena JR, Lendl B (2004) Raman spectroscopy
in chemical bioanalysis. Curr Opin Chem Biol
8:534–539
8. Schie IW, Huser T (2013) Methods and applications of Raman microspectroscopy to singlecell analysis. Appl Spectrosc 67:813–828
9. Swain R, Kemp S, Goldstraw P, Tetley TD,
Stevens MM (2010) Assessment of cell line
models of primary human cells by Raman spectral phenotyping. Biophys J 98:1703–1711
10. Nawaz H, Bonnier F, Knief P, Howe O, Lyng
FM, Meade AD, Byrne HJ (2010) Evaluation
of the potential of Raman microspectroscopy
for prediction of chemotherapeutic response to
cisplatin in lung adenocarcinoma. Analyst
135:3070–3076
11. Harkness L, Novikov SM, Beermann J, Bozhevolnyi SI, Kassem M (2012) Identification of
abnormal stem cells using Raman spectroscopy.
Stem Cells Dev 21:2152–2159
12. Li R, Yu G, Azarin SM, Hubel A (2018) Freezing responses in DMSO-based cryopreservation of human iPS cells: aggregates versus
single cells. Tissue Eng Part C Methods
24:289–299
13. Pollock K, Yu G, Moller-Trane R, Koran M,
Dosa PI, McKenna DH, Hubel A (2016)
Combinations of osmolytes, including monosaccharides, disaccharides, and sugar alcohols
act in concert during cryopreservation to
improve mesenchymal stromal cell survival.
Tissue Eng Part C Methods 22:999–1008
14. Yu G, Li R, Hubel A (2018) Interfacial interactions of sucrose during cryopreservation
detected by Raman spectroscopy. Langmuir
35:7388–7395
15. Bailey TL, Wang M, Solocinski J, Nathan BP,
Chakraborty N, Menze MA (2015) Protective
effects of osmolytes in cryopreserving adherent
neuroblastoma (Neuro-2a) cells. Cryobiology
71:472–480
16. Solocinski J, Osgood Q, Wang M, Connolly A,
Menze MA, Chakraborty N (2017) Effect of
trehalose as an additive to dimethyl sulfoxide
solutions on ice formation, cellular viability,
and metabolism. Cryobiology 75:134–143
17. Pi C, Yu G, Dosa PI, Hubel A (2019) Characterizing modes of action and interaction for
multicomponent osmolyte solutions on Jurkat
cells. Biotechnol Bioeng 116:631–643
18. Kreiner-Møller A, Stracke F, Zimmermann H
(2013) Confocal raman microscopy as a
non-invasive tool to investigate the phase composition of frozen complex cryopreservation
media. Cryo Letters 34:248–254
19. Kreiner-Møller A, Stracke F, Zimmermann H
(2014) Hydrohalite spatial distribution in frozen cell cultures measured using confocal
Raman microscopy. Cryobiology 69:41–47
20. Mathlouthi M, Luu DV (1980) Laser-raman
spectra of d-glucose and sucrose in aqueous
solution. Carbohydr Res 81:203–212
21. Brizuela AB, Bichara LC, Romano E,
Yurquina A, Locatelli S, Branda ´n SA (2012) A
complete characterization of the vibrational
spectra
of
sucrose.
Carbohydr
Res
361:212–218
22. Toner M (1993) Nucleation of ice crystals
inside biological cells. Adv Low Temperature
Biol 2:1–51
23. Kann B, Offerhaus HL, Windbergs M, Otto C
(2015) Raman microscopy for cellular investigations - from single cell imaging to drug carrier uptake visualization. Adv Drug Deliv Rev
89:71–90
Raman Cryomicroscopic Imaging
361
in chemical bioanalysis. Curr Opin Chem Biol
8:534–539
8. Schie IW, Huser T (2013) Methods and applications of Raman microspectroscopy to singlecell analysis. Appl Spectrosc 67:813–828
9. Swain R, Kemp S, Goldstraw P, Tetley TD,
Stevens MM (2010) Assessment of cell line
models of primary human cells by Raman spectral phenotyping. Biophys J 98:1703–1711
10. Nawaz H, Bonnier F, Knief P, Howe O, Lyng
FM, Meade AD, Byrne HJ (2010) Evaluation
of the potential of Raman microspectroscopy
for prediction of chemotherapeutic response to
cisplatin in lung adenocarcinoma. Analyst
135:3070–3076
11. Harkness L, Novikov SM, Beermann J, Bozhevolnyi SI, Kassem M (2012) Identification of
abnormal stem cells using Raman spectroscopy.
Stem Cells Dev 21:2152–2159
12. Li R, Yu G, Azarin SM, Hubel A (2018) Freezing responses in DMSO-based cryopreservation of human iPS cells: aggregates versus
single cells. Tissue Eng Part C Methods
24:289–299
13. Pollock K, Yu G, Moller-Trane R, Koran M,
Dosa PI, McKenna DH, Hubel A (2016)
Combinations of osmolytes, including monosaccharides, disaccharides, and sugar alcohols
act in concert during cryopreservation to
improve mesenchymal stromal cell survival.
Tissue Eng Part C Methods 22:999–1008
14. Yu G, Li R, Hubel A (2018) Interfacial interactions of sucrose during cryopreservation
detected by Raman spectroscopy. Langmuir
35:7388–7395
15. Bailey TL, Wang M, Solocinski J, Nathan BP,
Chakraborty N, Menze MA (2015) Protective
effects of osmolytes in cryopreserving adherent
neuroblastoma (Neuro-2a) cells. Cryobiology
71:472–480
16. Solocinski J, Osgood Q, Wang M, Connolly A,
Menze MA, Chakraborty N (2017) Effect of
trehalose as an additive to dimethyl sulfoxide
solutions on ice formation, cellular viability,
and metabolism. Cryobiology 75:134–143
17. Pi C, Yu G, Dosa PI, Hubel A (2019) Characterizing modes of action and interaction for
multicomponent osmolyte solutions on Jurkat
cells. Biotechnol Bioeng 116:631–643
18. Kreiner-Møller A, Stracke F, Zimmermann H
(2013) Confocal raman microscopy as a
non-invasive tool to investigate the phase composition of frozen complex cryopreservation
media. Cryo Letters 34:248–254
19. Kreiner-Møller A, Stracke F, Zimmermann H
(2014) Hydrohalite spatial distribution in frozen cell cultures measured using confocal
Raman microscopy. Cryobiology 69:41–47
20. Mathlouthi M, Luu DV (1980) Laser-raman
spectra of d-glucose and sucrose in aqueous
solution. Carbohydr Res 81:203–212
21. Brizuela AB, Bichara LC, Romano E,
Yurquina A, Locatelli S, Branda ´n SA (2012) A
complete characterization of the vibrational
spectra
of
sucrose.
Carbohydr
Res
361:212–218
22. Toner M (1993) Nucleation of ice crystals
inside biological cells. Adv Low Temperature
Biol 2:1–51
23. Kann B, Offerhaus HL, Windbergs M, Otto C
(2015) Raman microscopy for cellular investigations - from single cell imaging to drug carrier uptake visualization. Adv Drug Deliv Rev
89:71–90
Raman Cryomicroscopic Imaging
361