Acknowledgments
This work is supported by funding from the German Research
Foundation (DFG, Deutsche Forschungsgemeinschaft) via grants
WO1735/6-2 and SI1462/4-2.
References
1. Wolkers WF, Hoekstra FA (2003) In situ FTIR
assessment of desiccation-tolerant tissues.
Spectroscopy 17:297–313
2. Wolkers WF, Oldenhof H (2010) In situ FTIR
studies on mammalian cells. Spectroscopy
24:525–534
3. Wolkers WF, Balasubramanian SK, Ongstad
EL, Zec H, Bischof JC (2007) Effects of freezing on membranes and proteins in LNCaP
prostate tumor cells. Biochim Biophys Acta
1768:728–736
4. Oldenhof H, Friedel K, Sieme H,
Glasmacher B, Wolkers WF (2010) Freezinginduced membrane phase changes and water
transport in Stallion sperm: a Fourier transform
infrared spectroscopy study. Cryobiology
61:115–122
5. Sydykov B, Oldenhof H, Sieme H, Wolkers WF
(2017) Hydrogen bonding interactions and
enthalpy relaxation in sugar/protein glasses. J
Pharm Sci 106:761–769
6. Wang S, Oldenhof H, Dai X, Haverich A,
Hilfiker A, Harder M, Wolkers WF (2014)
Protein stability in stored decellularized heart
valve scaffolds and diffusion kinetics of protective molecules. Biochim Biophys Acta
1844:430–438
7. Wang S, Oldenhof H, Goecke T, Ramm R,
Harder M, Haverich A, Hilfiker A, Wolkers
WF (2015) Sucrose diffusion in decellularized
heart valves for freeze-drying. Tissue Eng Part
C Methods 21:922–931
8. Va ´squez-Rivera A, Sommer KK, Oldenhof H,
Higgins AZ, Brockbank KGM, Hilfiker A,
Wolkers WF (2018) Simultaneous monitoring
of different vitrification solution components
permeating into tissues. Analyst 43:420–428
9. Wolkers WF, Oldenhof H, Glasmacher B
(2010) Effect of trehalose on dehydration
kinetics of phospholipid vesicles, as measured
in real time using ATR infrared spectroscopy.
Cryobiology 61:108–114
10. Mantsch HH, McElhaney RN (1991) Phospholipid phase transitions in model and
biological membranes as studied by infrared
spectroscopy. Chem Phys Lipids 57:213–226
11. Goormaghtigh E, Cabiaux V, Ruysschaert JM
(1994) Determination of soluble and membrane protein structure by Fourier transform
infrared spectroscopy. I. Assignments and
model
compounds.
Subcell
Biochem
23:329–362
12. Wolkers WF, Oldenhof H, Alberda M, Hoekstra FA (1998) A Fourier transform infrared
microspectroscopy study of sugar glasses: application to anhydrobiotic higher plant cells. Biochim Biophys Acta 1379:83–96
13. O’Brien FEM (1948) The control of humidity
by saturated salt solutions. J Sci Instrum
25:73–76
14. Akhoondi M, Oldenhof H, Sieme H, Wolkers
WF (2012) Freezing-induced removal of water
from phospholipid head groups in biomembranes. Biomed Spectrosc Imaging 1:293–302
15. Oldenhof H, Akhoondi M, Sieme H, Wolkers
WF (2013) Use of Fourier transform infrared
spectroscopy to study membrane properties of
cells at subzero temperatures. Biomed Spectrosc Imaging 2:83–90
In situ Infrared Spectroscopy
349
This work is supported by funding from the German Research
Foundation (DFG, Deutsche Forschungsgemeinschaft) via grants
WO1735/6-2 and SI1462/4-2.
References
1. Wolkers WF, Hoekstra FA (2003) In situ FTIR
assessment of desiccation-tolerant tissues.
Spectroscopy 17:297–313
2. Wolkers WF, Oldenhof H (2010) In situ FTIR
studies on mammalian cells. Spectroscopy
24:525–534
3. Wolkers WF, Balasubramanian SK, Ongstad
EL, Zec H, Bischof JC (2007) Effects of freezing on membranes and proteins in LNCaP
prostate tumor cells. Biochim Biophys Acta
1768:728–736
4. Oldenhof H, Friedel K, Sieme H,
Glasmacher B, Wolkers WF (2010) Freezinginduced membrane phase changes and water
transport in Stallion sperm: a Fourier transform
infrared spectroscopy study. Cryobiology
61:115–122
5. Sydykov B, Oldenhof H, Sieme H, Wolkers WF
(2017) Hydrogen bonding interactions and
enthalpy relaxation in sugar/protein glasses. J
Pharm Sci 106:761–769
6. Wang S, Oldenhof H, Dai X, Haverich A,
Hilfiker A, Harder M, Wolkers WF (2014)
Protein stability in stored decellularized heart
valve scaffolds and diffusion kinetics of protective molecules. Biochim Biophys Acta
1844:430–438
7. Wang S, Oldenhof H, Goecke T, Ramm R,
Harder M, Haverich A, Hilfiker A, Wolkers
WF (2015) Sucrose diffusion in decellularized
heart valves for freeze-drying. Tissue Eng Part
C Methods 21:922–931
8. Va ´squez-Rivera A, Sommer KK, Oldenhof H,
Higgins AZ, Brockbank KGM, Hilfiker A,
Wolkers WF (2018) Simultaneous monitoring
of different vitrification solution components
permeating into tissues. Analyst 43:420–428
9. Wolkers WF, Oldenhof H, Glasmacher B
(2010) Effect of trehalose on dehydration
kinetics of phospholipid vesicles, as measured
in real time using ATR infrared spectroscopy.
Cryobiology 61:108–114
10. Mantsch HH, McElhaney RN (1991) Phospholipid phase transitions in model and
biological membranes as studied by infrared
spectroscopy. Chem Phys Lipids 57:213–226
11. Goormaghtigh E, Cabiaux V, Ruysschaert JM
(1994) Determination of soluble and membrane protein structure by Fourier transform
infrared spectroscopy. I. Assignments and
model
compounds.
Subcell
Biochem
23:329–362
12. Wolkers WF, Oldenhof H, Alberda M, Hoekstra FA (1998) A Fourier transform infrared
microspectroscopy study of sugar glasses: application to anhydrobiotic higher plant cells. Biochim Biophys Acta 1379:83–96
13. O’Brien FEM (1948) The control of humidity
by saturated salt solutions. J Sci Instrum
25:73–76
14. Akhoondi M, Oldenhof H, Sieme H, Wolkers
WF (2012) Freezing-induced removal of water
from phospholipid head groups in biomembranes. Biomed Spectrosc Imaging 1:293–302
15. Oldenhof H, Akhoondi M, Sieme H, Wolkers
WF (2013) Use of Fourier transform infrared
spectroscopy to study membrane properties of
cells at subzero temperatures. Biomed Spectrosc Imaging 2:83–90
In situ Infrared Spectroscopy
349