5. Coring device (e.g., 10-mm diameter sharp coring device,
custom-made by Argyll Innovations Inc., Edmonton, Alberta).
6. Coring puncher (e.g., Stiletto titanium 18-in. framer).
7. Plastic cooler (e.g., 28 quart cooler).
8. Dissection board (e.g., 18 Â 24 in. cutting board).
9. Absorbent underpads (e.g., 36 Â 20 in.).
10. 70% ethanol (prepared from 100% ethanol, anhydrous ethyl
alcohol).
11. Paper towels.
12. 4
C fridge.
2.2 Cryoprotective
Agent Permeation into
Tissue
and Osmometric
Measurement
1. Incubator shaker.
2. Orbital mini shaker (e.g., VWR
® Mini shaker).
3. Thermocouple thermometer (e.g., Cole-Parmer, thermocouple thermometer dual type JKTE).
4. Thermocouple probe (e.g., Cole-Parmer, Digi-Sense type K
high-temperature flexible thermocouple probe).
5. Digital timer.
6. Stirrer (e.g., VWR
® Mini stirrer).
7. Large lab beaker (e.g., 250 mL).
8. Small lab beaker (e.g., 30 mL).
9. Sterile pipettes (e.g., 5 and 25 mL sterile serological pipettes).
10. Pipette controller.
11. Adjustable pipette set (e.g., 30–300 μL Finnpipette™ manual
pipettes).
12. Sterile pipette tips.
13. Sterile
microcentrifuge
tubes
(e.g.,
1.5-mL
microcentrifuge tube).
14. Multi-well plate for CPA permeation (e.g., 6-well tissue culture
plate).
15. Parafilm for sealing the plates.
16. Balance (e.g., analytical balance).
17. Osmometer (Osmette™ Micro-Osmette osmometer, Precision Systems Inc.).
18. Osmometer test tube (50-μL disposable test tube, Precision
Systems Inc.).
19. Standard buffer for osmometer (Con-Trol 290, Osmometer
reference standards, Precision Systems Inc.).
20. Kimwipes.
Osmometric Measurements of Cryoprotective Agent Permeation
305
custom-made by Argyll Innovations Inc., Edmonton, Alberta).
6. Coring puncher (e.g., Stiletto titanium 18-in. framer).
7. Plastic cooler (e.g., 28 quart cooler).
8. Dissection board (e.g., 18 Â 24 in. cutting board).
9. Absorbent underpads (e.g., 36 Â 20 in.).
10. 70% ethanol (prepared from 100% ethanol, anhydrous ethyl
alcohol).
11. Paper towels.
12. 4
C fridge.
2.2 Cryoprotective
Agent Permeation into
Tissue
and Osmometric
Measurement
1. Incubator shaker.
2. Orbital mini shaker (e.g., VWR
® Mini shaker).
3. Thermocouple thermometer (e.g., Cole-Parmer, thermocouple thermometer dual type JKTE).
4. Thermocouple probe (e.g., Cole-Parmer, Digi-Sense type K
high-temperature flexible thermocouple probe).
5. Digital timer.
6. Stirrer (e.g., VWR
® Mini stirrer).
7. Large lab beaker (e.g., 250 mL).
8. Small lab beaker (e.g., 30 mL).
9. Sterile pipettes (e.g., 5 and 25 mL sterile serological pipettes).
10. Pipette controller.
11. Adjustable pipette set (e.g., 30–300 μL Finnpipette™ manual
pipettes).
12. Sterile pipette tips.
13. Sterile
microcentrifuge
tubes
(e.g.,
1.5-mL
microcentrifuge tube).
14. Multi-well plate for CPA permeation (e.g., 6-well tissue culture
plate).
15. Parafilm for sealing the plates.
16. Balance (e.g., analytical balance).
17. Osmometer (Osmette™ Micro-Osmette osmometer, Precision Systems Inc.).
18. Osmometer test tube (50-μL disposable test tube, Precision
Systems Inc.).
19. Standard buffer for osmometer (Con-Trol 290, Osmometer
reference standards, Precision Systems Inc.).
20. Kimwipes.
Osmometric Measurements of Cryoprotective Agent Permeation
305