28. Alternatively, if one does not want the cryomicroscope stage to
be permanently installed on the microscope, this step can be
performed at the beginning of the experimental setup procedure (i.e., before completing any of the steps in Subheading
3.3).
29. Alternatively, if one does not want the camera to be permanently installed on the microscope, this step can be performed
at the beginning of the experimental setup procedure (i.e.,
before completing any of the steps in Subheading 3.3).
30. If the camera body or the microscope camera port cannot be
configured to accept F-mount hardware, a C-mount adapter
can be used as an alternative. Nonetheless, because high-speed
imaging sensors typically have large dimensions, an F-mount is
preferable, because it provides a wider field of view (and thus
prevents vignetting).
31. Depending on the weight of the camera model used, the
mechanical strength of the microscope stand, and the configuration of the camera port and coupling adapter, it may be
necessary to support the weight of the high-speed camera
(to prevent undue mechanical stress on the microscope components). For example, a tripod or a scissor jack platform may
be used to counteract the forces and torques resulting from the
camera weight.
32. Confirm that there is sufficient storage space on the hard drive
to hold the video files that will be generated. If there is insufficient space on the computer hard drive, delete any videos from
previous experiments, after first backing up the files to a data
archive.
33. Before establishing a connection to the system controller the
first time, software settings should be configured as described
in the Linksys32 User Guide. In particular, go to “Temperature Controller” under the “Setup” menu, select the “TempControl” entry in the navigation tree, and then specify the
computer COM port number to which the controller unit’s
serial communication cable was connected (in step 6 of Subheading 3.2). In the same setup window, check the box labeled
“Use profile,” and then close the window. Next, right-click in
the Data Capture toolbar to bring up the “Capture Setup”
window, and set the option “Control from capture control
start stop,” and then close the setup window.
34. The sequence is important. The system controller must be
powered up after the LNP is turned on, or else the controller
will not recognize the pump. The “OK” button in the software
prompt must not be clicked before the hardware is powered up,
or else the software will not be able to establish communication
with the controller unit.
250
Jens O. M. Karlsson
be permanently installed on the microscope, this step can be
performed at the beginning of the experimental setup procedure (i.e., before completing any of the steps in Subheading
3.3).
29. Alternatively, if one does not want the camera to be permanently installed on the microscope, this step can be performed
at the beginning of the experimental setup procedure (i.e.,
before completing any of the steps in Subheading 3.3).
30. If the camera body or the microscope camera port cannot be
configured to accept F-mount hardware, a C-mount adapter
can be used as an alternative. Nonetheless, because high-speed
imaging sensors typically have large dimensions, an F-mount is
preferable, because it provides a wider field of view (and thus
prevents vignetting).
31. Depending on the weight of the camera model used, the
mechanical strength of the microscope stand, and the configuration of the camera port and coupling adapter, it may be
necessary to support the weight of the high-speed camera
(to prevent undue mechanical stress on the microscope components). For example, a tripod or a scissor jack platform may
be used to counteract the forces and torques resulting from the
camera weight.
32. Confirm that there is sufficient storage space on the hard drive
to hold the video files that will be generated. If there is insufficient space on the computer hard drive, delete any videos from
previous experiments, after first backing up the files to a data
archive.
33. Before establishing a connection to the system controller the
first time, software settings should be configured as described
in the Linksys32 User Guide. In particular, go to “Temperature Controller” under the “Setup” menu, select the “TempControl” entry in the navigation tree, and then specify the
computer COM port number to which the controller unit’s
serial communication cable was connected (in step 6 of Subheading 3.2). In the same setup window, check the box labeled
“Use profile,” and then close the window. Next, right-click in
the Data Capture toolbar to bring up the “Capture Setup”
window, and set the option “Control from capture control
start stop,” and then close the setup window.
34. The sequence is important. The system controller must be
powered up after the LNP is turned on, or else the controller
will not recognize the pump. The “OK” button in the software
prompt must not be clicked before the hardware is powered up,
or else the software will not be able to establish communication
with the controller unit.
250
Jens O. M. Karlsson