3. Glycerin monostearate and CTAB are used as positive-charged
surfactant, and SDS is used as negative-charged surfactant.
4. In the pharmacokinetic experiment, due to the low content of
CC, the addition of the internal standard nitrendipine during
the measurement can improve the accuracy and precision of the
measurement results. The basis of nitrendipine as the standard
of CC internal standard: Nitrendipine is similar to CC in
physical and chemical properties; it does not react with CC
sample; it has similar peak time with CC and can be completely
separated from components in CC sample.
5. Adding glacial acetic acid to the mobile phase can improve the
peak shape of CC.
6. Light-sensitive CC should be preserved in the dark with
aluminum foil.
7. Impurities and bubbles are removed by 0.22 μm organic filtration and sonication, respectively.
8. Encapsulation ratio and drug content of CC@CHLNV are
influenced by the ratios of amounts of glycerin monostearate,
isopropyl palmitate, Lipoid-S 75, and surfactant. In addition,
the size and zeta potential of the nanoparticles may be changed
by above materials. The ratios of materials in “Methods” are
the optimal conditions. The optimal prescription is optimized
by central composite design-response surface methodology to
achieve the maximum encapsulation rate and drug loading.
9. Rotating evaporation time is related to the temperature,
amounts of absolute ethanol and lipid materials. In general,
20 mL absolute ethanol and 200 mg lipid materials at 30
C
under hypobaric conditions for 40 min.
10. Under the above conditions, the peak times of CC and nitrendipine are 5.8 min and 7.6 min, respectively, and the peak shape
is good and there is no interference. Due to the complex
composition of the blood samples, the relatively high content
of impurities, and the low concentration of CC in the blood
samples, and the column is washed with mobile phase for 1–2 h
after 15–20 CC samples are measured (the specific rinsing time
is related to the sample and the column). This behavior may
improve the accuracy of CC content determination and
increase the accuracy of calculating CC bioavailability.
11. Oscillating for 10 min allow the drug to dissolve better.
12. The error is small when the absorbance is between 0.2 and 0.8.
If it is beyond this range, adjust the number of inoculated cells
and incubation time.
66
Xuemei Xie et al.
surfactant, and SDS is used as negative-charged surfactant.
4. In the pharmacokinetic experiment, due to the low content of
CC, the addition of the internal standard nitrendipine during
the measurement can improve the accuracy and precision of the
measurement results. The basis of nitrendipine as the standard
of CC internal standard: Nitrendipine is similar to CC in
physical and chemical properties; it does not react with CC
sample; it has similar peak time with CC and can be completely
separated from components in CC sample.
5. Adding glacial acetic acid to the mobile phase can improve the
peak shape of CC.
6. Light-sensitive CC should be preserved in the dark with
aluminum foil.
7. Impurities and bubbles are removed by 0.22 μm organic filtration and sonication, respectively.
8. Encapsulation ratio and drug content of CC@CHLNV are
influenced by the ratios of amounts of glycerin monostearate,
isopropyl palmitate, Lipoid-S 75, and surfactant. In addition,
the size and zeta potential of the nanoparticles may be changed
by above materials. The ratios of materials in “Methods” are
the optimal conditions. The optimal prescription is optimized
by central composite design-response surface methodology to
achieve the maximum encapsulation rate and drug loading.
9. Rotating evaporation time is related to the temperature,
amounts of absolute ethanol and lipid materials. In general,
20 mL absolute ethanol and 200 mg lipid materials at 30
C
under hypobaric conditions for 40 min.
10. Under the above conditions, the peak times of CC and nitrendipine are 5.8 min and 7.6 min, respectively, and the peak shape
is good and there is no interference. Due to the complex
composition of the blood samples, the relatively high content
of impurities, and the low concentration of CC in the blood
samples, and the column is washed with mobile phase for 1–2 h
after 15–20 CC samples are measured (the specific rinsing time
is related to the sample and the column). This behavior may
improve the accuracy of CC content determination and
increase the accuracy of calculating CC bioavailability.
11. Oscillating for 10 min allow the drug to dissolve better.
12. The error is small when the absorbance is between 0.2 and 0.8.
If it is beyond this range, adjust the number of inoculated cells
and incubation time.
66
Xuemei Xie et al.