Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
ix
PART I BACTERIAL VECTORS
1 Improving E. coli Bactofection by Expression of Bacteriophage
ΦX174 Gene E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3
Dongwon Park, Andrew Hill, and Blaine A. Pfeifer
2 Improved DNA Delivery Efficiency of Bacterial Vectors
by Co-Delivery with Exogenous Lipid and Antimicrobial Reagents . . . . . . . . . . . 15
Andrew N. Osahor and Kumaran Narayanan
3 Visualization of Bacteria-Mediated Gene Delivery Using
High-Resolution Electron and Confocal Microscopy . . . . . . . . . . . . . . . . . . . . . . . . 29
Andrew N. Osahor, Allan Wee Ren Ng, and Kumaran Narayanan
PART II NANOPARTICLES
4 Assessing Nucleic Acid: Cationic Nanoparticle Interaction
for Gene Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Moganavelli Singh
5 Catanionic Hybrid Lipid Nanovesicles for Improved Bioavailability
and Efficacy of Chemotherapeutic Drugs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Xuemei Xie, Dan He, Yan Wu, Tingting Wang, Cailing Zhong,
and Jingqing Zhang
6 Magnetic Nanoparticles as a Carrier of dsRNA for Gene Therapy . . . . . . . . . . . . . 69
Małgorzata Grabowska, Bartosz F. Grzes ´kowiak, Katarzyna Rolle,
and Radosław Mr owczyn ´ ski
PART III PEPTIDE VEHICLES
7 Generation of Functional Insulin-Producing Cells from Mouse
Embryonic Stem Cells Through Protein Transduction
of Transcription Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Taku Kaitsuka and Kazuhito Tomizawa
8 Cardiac Targeting Peptide: From Identification to Validation
to Mechanism of Transduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Kyle S. Feldman, Maria P. Pavlou, and Maliha Zahid
9 Lactoferricin-Derived L5a Cell-Penetrating Peptide for Delivery
of DNA into Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Natalie J. Holl, Moumita Dey, Yue-Wern Huang, Shiow-Her Chiou,
and Han-Jung Lee
vii
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
ix
PART I BACTERIAL VECTORS
1 Improving E. coli Bactofection by Expression of Bacteriophage
ΦX174 Gene E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3
Dongwon Park, Andrew Hill, and Blaine A. Pfeifer
2 Improved DNA Delivery Efficiency of Bacterial Vectors
by Co-Delivery with Exogenous Lipid and Antimicrobial Reagents . . . . . . . . . . . 15
Andrew N. Osahor and Kumaran Narayanan
3 Visualization of Bacteria-Mediated Gene Delivery Using
High-Resolution Electron and Confocal Microscopy . . . . . . . . . . . . . . . . . . . . . . . . 29
Andrew N. Osahor, Allan Wee Ren Ng, and Kumaran Narayanan
PART II NANOPARTICLES
4 Assessing Nucleic Acid: Cationic Nanoparticle Interaction
for Gene Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Moganavelli Singh
5 Catanionic Hybrid Lipid Nanovesicles for Improved Bioavailability
and Efficacy of Chemotherapeutic Drugs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Xuemei Xie, Dan He, Yan Wu, Tingting Wang, Cailing Zhong,
and Jingqing Zhang
6 Magnetic Nanoparticles as a Carrier of dsRNA for Gene Therapy . . . . . . . . . . . . . 69
Małgorzata Grabowska, Bartosz F. Grzes ´kowiak, Katarzyna Rolle,
and Radosław Mr owczyn ´ ski
PART III PEPTIDE VEHICLES
7 Generation of Functional Insulin-Producing Cells from Mouse
Embryonic Stem Cells Through Protein Transduction
of Transcription Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Taku Kaitsuka and Kazuhito Tomizawa
8 Cardiac Targeting Peptide: From Identification to Validation
to Mechanism of Transduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Kyle S. Feldman, Maria P. Pavlou, and Maliha Zahid
9 Lactoferricin-Derived L5a Cell-Penetrating Peptide for Delivery
of DNA into Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Natalie J. Holl, Moumita Dey, Yue-Wern Huang, Shiow-Her Chiou,
and Han-Jung Lee
vii