treatments including treatment with invasive media, PBS
washes, and gentamicin treatment, without invasion using
E. coli.
Acknowledgments
This work was supported by a MOSTI eScience grant 02-02-10SF0252 from the Ministry of Science, Technology and Innovation,
Malaysia, to K.N. A.N.O. was supported by a Higher Degree by
Research Scholarships from Monash University Malaysia.
References
1. Celec P, Gardlik R (2017) Gene therapy using
bacterial vectors. Front Biosci (Landmark Ed)
22:81–95
2. Guzman-Herrador DL, Steiner S, Alperi A,
Gonzalez-Prieto C, Roy CR, Llosa M (2017)
DNA delivery and genomic integration into
mammalian target cells through type IV a and
B secretion systems of human pathogens. Front
Microbiol 8:1503. https://doi.org/10.3389/
fmicb.2017.01503
3. Cronin M, Stanton RM, Francis KP, Tangney
M (2012) Bacterial vectors for imaging and
cancer gene therapy: a review. Cancer Gene
Ther 19(11):731–740. https://doi.org/10.
1038/cgt.2012.59
4. Dalby B, Cates S, Harris A, Ohki EC, Tilkins
ML, Price PJ, Ciccarone VC (2004) Advanced
transfection with Lipofectamine 2000 reagent:
primary neurons, siRNA, and high-throughput
applications. Methods 33(2):95–103. https://
doi.org/10.1016/j.ymeth.2003.11.023
5. Huh SH, Do HJ, Lim HY, Kim DK, Choi SJ,
Song H, Kim NH, Park JK, Chang WK, Chung
HM, Kim JH (2007) Optimization of 25 kDa
linear polyethylenimine for efficient gene delivery. Biologicals 35(3):165–171. https://doi.
org/10.1016/j.biologicals.2006.08.004
6. Palffy R, Gardlik R, Hodosy J, Behuliak M,
Resko P, Radvansky J, Celec P (2006) Bacteria
in gene therapy: bactofection versus alternative
gene therapy. Gene Ther 13(2):101–105.
https://doi.org/10.1038/sj.gt.3302635
7. Lehouritis P, Springer C, Tangney M (2013)
Bacterial-directed enzyme prodrug therapy. J
Control Release 170(1):120–131. https://
doi.org/10.1016/j.jconrel.2013.05.005
8. da Silva AJ, Zangirolami TC, Novo-Mansur
MT, Giordano Rde C, Martins EA (2014)
Live bacterial vaccine vectors: an overview.
Braz J Microbiol 45(4):1117–1129
9. Souders NC, Verch T, Paterson Y (2006) In
vivo bactofection: listeria can function as a
DNA-cancer vaccine. DNA Cell Biol 25
(3):142–151. https://doi.org/10.1089/dna.
2006.25.142
10. Jones CH, Rane S, Patt E, Ravikrishnan A,
Chen CK, Cheng C, Pfeifer BA (2013) Polymyxin B treatment improves bactofection efficacy and reduces cytotoxicity. Mol Pharm 10
(11):4301–4308. https://doi.org/10.1021/
mp4003927
11. Narayanan K, Lee CW, Radu A, Sim EU
(2013) Escherichia coli bactofection using lipofectamine. Anal Biochem 439(2):142–144.
https://doi.org/10.1016/j.ab.2013.04.010
12. Hill AB, Chen M, Chen CK, Pfeifer BA, Jones
CH (2016) Overcoming gene-delivery hurdles: physiological considerations for nonviral
vectors. Trends Biotechnol 34(2):91–105.
https://doi.org/10.1016/j.tibtech.2015.11.
004
13. Hart SL, Arancibia-Carcamo CV, Wolfert MA,
Mailhos C, O’Reilly NJ, Ali RR, Coutelle C,
George AJ, Harbottle RP, Knight AM, Larkin
DF, Levinsky RJ, Seymour LW, Thrasher AJ,
Kinnon C (1998) Lipid-mediated enhancement of transfection by a nonviral integrintargeting vector. Hum Gene Ther 9
(4):575–585. https://doi.org/10.1089/hum.
1998.9.4-575
14. Debaisieux S, Rayne F, Yezid H, Beaumelle B
(2012) The ins and outs of HIV-1 tat. Traffic
13(3):355–363. https://doi.org/10.1111/j.
1600-0854.2011.01286.x
15. Pieper GM, Olds CL, Bub JD, Lindholm PF
(2002) Transfection of human endothelial cells
with HIV-1 tat gene activates NF-kappa B and
enhances monocyte adhesion. Am J Physiol
Heart Circ Physiol 283(6):H2315–H2321.
https://doi.org/10.1152/ajpheart.00469.
2002
26
Andrew N. Osahor and Kumaran Narayanan
washes, and gentamicin treatment, without invasion using
E. coli.
Acknowledgments
This work was supported by a MOSTI eScience grant 02-02-10SF0252 from the Ministry of Science, Technology and Innovation,
Malaysia, to K.N. A.N.O. was supported by a Higher Degree by
Research Scholarships from Monash University Malaysia.
References
1. Celec P, Gardlik R (2017) Gene therapy using
bacterial vectors. Front Biosci (Landmark Ed)
22:81–95
2. Guzman-Herrador DL, Steiner S, Alperi A,
Gonzalez-Prieto C, Roy CR, Llosa M (2017)
DNA delivery and genomic integration into
mammalian target cells through type IV a and
B secretion systems of human pathogens. Front
Microbiol 8:1503. https://doi.org/10.3389/
fmicb.2017.01503
3. Cronin M, Stanton RM, Francis KP, Tangney
M (2012) Bacterial vectors for imaging and
cancer gene therapy: a review. Cancer Gene
Ther 19(11):731–740. https://doi.org/10.
1038/cgt.2012.59
4. Dalby B, Cates S, Harris A, Ohki EC, Tilkins
ML, Price PJ, Ciccarone VC (2004) Advanced
transfection with Lipofectamine 2000 reagent:
primary neurons, siRNA, and high-throughput
applications. Methods 33(2):95–103. https://
doi.org/10.1016/j.ymeth.2003.11.023
5. Huh SH, Do HJ, Lim HY, Kim DK, Choi SJ,
Song H, Kim NH, Park JK, Chang WK, Chung
HM, Kim JH (2007) Optimization of 25 kDa
linear polyethylenimine for efficient gene delivery. Biologicals 35(3):165–171. https://doi.
org/10.1016/j.biologicals.2006.08.004
6. Palffy R, Gardlik R, Hodosy J, Behuliak M,
Resko P, Radvansky J, Celec P (2006) Bacteria
in gene therapy: bactofection versus alternative
gene therapy. Gene Ther 13(2):101–105.
https://doi.org/10.1038/sj.gt.3302635
7. Lehouritis P, Springer C, Tangney M (2013)
Bacterial-directed enzyme prodrug therapy. J
Control Release 170(1):120–131. https://
doi.org/10.1016/j.jconrel.2013.05.005
8. da Silva AJ, Zangirolami TC, Novo-Mansur
MT, Giordano Rde C, Martins EA (2014)
Live bacterial vaccine vectors: an overview.
Braz J Microbiol 45(4):1117–1129
9. Souders NC, Verch T, Paterson Y (2006) In
vivo bactofection: listeria can function as a
DNA-cancer vaccine. DNA Cell Biol 25
(3):142–151. https://doi.org/10.1089/dna.
2006.25.142
10. Jones CH, Rane S, Patt E, Ravikrishnan A,
Chen CK, Cheng C, Pfeifer BA (2013) Polymyxin B treatment improves bactofection efficacy and reduces cytotoxicity. Mol Pharm 10
(11):4301–4308. https://doi.org/10.1021/
mp4003927
11. Narayanan K, Lee CW, Radu A, Sim EU
(2013) Escherichia coli bactofection using lipofectamine. Anal Biochem 439(2):142–144.
https://doi.org/10.1016/j.ab.2013.04.010
12. Hill AB, Chen M, Chen CK, Pfeifer BA, Jones
CH (2016) Overcoming gene-delivery hurdles: physiological considerations for nonviral
vectors. Trends Biotechnol 34(2):91–105.
https://doi.org/10.1016/j.tibtech.2015.11.
004
13. Hart SL, Arancibia-Carcamo CV, Wolfert MA,
Mailhos C, O’Reilly NJ, Ali RR, Coutelle C,
George AJ, Harbottle RP, Knight AM, Larkin
DF, Levinsky RJ, Seymour LW, Thrasher AJ,
Kinnon C (1998) Lipid-mediated enhancement of transfection by a nonviral integrintargeting vector. Hum Gene Ther 9
(4):575–585. https://doi.org/10.1089/hum.
1998.9.4-575
14. Debaisieux S, Rayne F, Yezid H, Beaumelle B
(2012) The ins and outs of HIV-1 tat. Traffic
13(3):355–363. https://doi.org/10.1111/j.
1600-0854.2011.01286.x
15. Pieper GM, Olds CL, Bub JD, Lindholm PF
(2002) Transfection of human endothelial cells
with HIV-1 tat gene activates NF-kappa B and
enhances monocyte adhesion. Am J Physiol
Heart Circ Physiol 283(6):H2315–H2321.
https://doi.org/10.1152/ajpheart.00469.
2002
26
Andrew N. Osahor and Kumaran Narayanan