2.2 E. coli Invasion
Enhancing Reagents
1. Lipofectamine 2000: Store at 4
C.
2. PULSin: Store at À20
C.
3. 10 mM Amantadine hydrochloride: Dissolve 18.7 mg in
10 mL dH 2 O (stock), working concentration of 50 μM (store
at À20
C).
Fig. 2 Attachment of invasive E. coli–lipid complexes to the membranes of HT1080 cells. Cells invaded with
E. coli complexed with Lipofectamine and PULSin observed using fluorescence microscopy at 20X magnification. Invasive E. coli (fluorescent red) expressing pUltraRFP-KM [18] uniformly adhere to cell membranes and
are distributed throughout the invaded cell population in E. coli–lipid complexes, but not when invasive E. coli
is used alone
18
Andrew N. Osahor and Kumaran Narayanan
Enhancing Reagents
1. Lipofectamine 2000: Store at 4
C.
2. PULSin: Store at À20
C.
3. 10 mM Amantadine hydrochloride: Dissolve 18.7 mg in
10 mL dH 2 O (stock), working concentration of 50 μM (store
at À20
C).
Fig. 2 Attachment of invasive E. coli–lipid complexes to the membranes of HT1080 cells. Cells invaded with
E. coli complexed with Lipofectamine and PULSin observed using fluorescence microscopy at 20X magnification. Invasive E. coli (fluorescent red) expressing pUltraRFP-KM [18] uniformly adhere to cell membranes and
are distributed throughout the invaded cell population in E. coli–lipid complexes, but not when invasive E. coli
is used alone
18
Andrew N. Osahor and Kumaran Narayanan