F
Fabry ................................................................................ 37
FACS, see Fluorescence activated cell sorting (FACS)
Fibers .................................................88, 93, 94, 184–190
Fluorescence activated cell sorting (FACS) .................. 29,
65, 99, 105, 132, 136, 205, 215, 217, 218, 224,
225, 228
Fluorescent ............................. 29, 32, 33, 36, 37, 64, 65,
67, 80, 105, 116–120, 214
G
Gel retardation assay ................. 52, 71, 74, 75, 115–117,
119, 120
Gene delivery..................................... 4, 5, 11, 16, 17, 24,
29–40, 43–54
Gene expression .................................7–9, 22, 23, 25, 79,
86, 97, 114, 117, 118, 120, 124, 125, 163
Gene therapy .............................. 3, 69–80, 114, 115, 194
Genome editing ..................................183–190, 194, 214
Genomic cleavage.........................................186, 188–190
Gentamicin .......................... 5, 19, 22, 26, 30, 31, 36, 39
Glutaraldehyde ................................................... 32, 37, 38
Green fluorescence protein (GFP) ......................... 29, 37,
94, 118, 190, 213–228
Gum ...................................................................... 175, 181
H
Hemocytometer ................ 11, 20, 21, 33, 164, 215–217
Hemoglobin E ..................................................... 193–210
Hemolysis assays................................................................ 9
Homology-directed repair (HDR) .................... 183, 194,
214, 224
Hybrids ............................................................. 57–67, 157
Hydrodynamic............................153, 160, 162, 164, 166
Hydrogels ............................................172–174, 179, 181
I
Indels .................................................................... 190, 214
Induced pluripotent stem cells (IPSCs).............. 193–210
Invasion, see E. coli
Ionotropic............................................173, 175–179, 181
Irradiation............................................. 38, 134, 174–176,
180, 187
L
Lactoferricin .................................................................. 114
L-Dopa ................................................................. 185, 187
Lentiviral........................................................................ 194
Ligands ............................................................98, 99, 102,
107–109, 111, 148
Lipids ..............................................15–26, 43, 44, 57–67,
147–149, 153, 156, 157, 189
Liposomes...............................................43, 44, 125, 147,
148, 150, 157
Listeria monocytogenes ....................................................... 3
Listeriolysin O (LLO)...............................................3–5, 9
Live/dead assay .........................................................72, 77
Luciferase.....................................................................5, 11
LyE, see Gene E
Lysis .............................................4, 9, 25, 87, 89, 90, 98,
103, 108, 109, 214, 227, 233
M
Mammalian cells
Eβ-iPSCs ............................................... 194, 195, 198,
199, 204, 207
GM02775..................................................... 33, 35, 37
H9C2...............................................99, 104, 105, 110
HEK 293 .............................................................17, 21
HeLa ................................................... 16, 17, 21, 156,
157, 159
HT1080....................................................... 16–19, 21,
30, 33, 35
K562 ............................................................... 213–227
Lewis lung cancer cells........................................ 61–65
Mouse embryonic stem cells .............................. 85–95
NK-92................................................... 15, 17, 18, 20,
225–227
RAW 264.7.................................................................. 4
Raji .................................................................. 213–227
SK-N-SH ...................................................... 30, 33, 35
3T3 ................................................101, 104, 105, 110
U-118 MG ................................................... 72, 76, 77
U2OS.EGFP cells .......................................... 185, 190
U251......................................................126–129, 132,
133, 136–140
U937......................................................153–156, 160,
161, 163, 164
Mass spectrometry (MS)....................................... 98, 109,
135, 233–239
Matrices ......................................... 59, 70, 173, 178–181,
196, 234
Membrane disruption ............................................ 4, 9, 13
Micronization ................................................................ 180
MicroRNA (miRNAs) ............................... 124, 125, 127,
128, 133, 135, 137–139, 184
Microscopy
confocal.............................................. 30–32, 111, 190
electron .................................................. 31, 45, 49, 54
fluorescence .........................................................29, 45
inverted ...................................................209, 215–217
MTT ...............................................................7, 12, 60, 62
Multiplicity of infection (MOI) ............................. 11, 12,
14, 24
Mutations ..................................193, 194, 196, 197, 199,
201, 204, 206, 209
242
BIO-CARRIER VECTORS: METHODS AND PROTOCOLS
Index
Fabry ................................................................................ 37
FACS, see Fluorescence activated cell sorting (FACS)
Fibers .................................................88, 93, 94, 184–190
Fluorescence activated cell sorting (FACS) .................. 29,
65, 99, 105, 132, 136, 205, 215, 217, 218, 224,
225, 228
Fluorescent ............................. 29, 32, 33, 36, 37, 64, 65,
67, 80, 105, 116–120, 214
G
Gel retardation assay ................. 52, 71, 74, 75, 115–117,
119, 120
Gene delivery..................................... 4, 5, 11, 16, 17, 24,
29–40, 43–54
Gene expression .................................7–9, 22, 23, 25, 79,
86, 97, 114, 117, 118, 120, 124, 125, 163
Gene therapy .............................. 3, 69–80, 114, 115, 194
Genome editing ..................................183–190, 194, 214
Genomic cleavage.........................................186, 188–190
Gentamicin .......................... 5, 19, 22, 26, 30, 31, 36, 39
Glutaraldehyde ................................................... 32, 37, 38
Green fluorescence protein (GFP) ......................... 29, 37,
94, 118, 190, 213–228
Gum ...................................................................... 175, 181
H
Hemocytometer ................ 11, 20, 21, 33, 164, 215–217
Hemoglobin E ..................................................... 193–210
Hemolysis assays................................................................ 9
Homology-directed repair (HDR) .................... 183, 194,
214, 224
Hybrids ............................................................. 57–67, 157
Hydrodynamic............................153, 160, 162, 164, 166
Hydrogels ............................................172–174, 179, 181
I
Indels .................................................................... 190, 214
Induced pluripotent stem cells (IPSCs).............. 193–210
Invasion, see E. coli
Ionotropic............................................173, 175–179, 181
Irradiation............................................. 38, 134, 174–176,
180, 187
L
Lactoferricin .................................................................. 114
L-Dopa ................................................................. 185, 187
Lentiviral........................................................................ 194
Ligands ............................................................98, 99, 102,
107–109, 111, 148
Lipids ..............................................15–26, 43, 44, 57–67,
147–149, 153, 156, 157, 189
Liposomes...............................................43, 44, 125, 147,
148, 150, 157
Listeria monocytogenes ....................................................... 3
Listeriolysin O (LLO)...............................................3–5, 9
Live/dead assay .........................................................72, 77
Luciferase.....................................................................5, 11
LyE, see Gene E
Lysis .............................................4, 9, 25, 87, 89, 90, 98,
103, 108, 109, 214, 227, 233
M
Mammalian cells
Eβ-iPSCs ............................................... 194, 195, 198,
199, 204, 207
GM02775..................................................... 33, 35, 37
H9C2...............................................99, 104, 105, 110
HEK 293 .............................................................17, 21
HeLa ................................................... 16, 17, 21, 156,
157, 159
HT1080....................................................... 16–19, 21,
30, 33, 35
K562 ............................................................... 213–227
Lewis lung cancer cells........................................ 61–65
Mouse embryonic stem cells .............................. 85–95
NK-92................................................... 15, 17, 18, 20,
225–227
RAW 264.7.................................................................. 4
Raji .................................................................. 213–227
SK-N-SH ...................................................... 30, 33, 35
3T3 ................................................101, 104, 105, 110
U-118 MG ................................................... 72, 76, 77
U2OS.EGFP cells .......................................... 185, 190
U251......................................................126–129, 132,
133, 136–140
U937......................................................153–156, 160,
161, 163, 164
Mass spectrometry (MS)....................................... 98, 109,
135, 233–239
Matrices ......................................... 59, 70, 173, 178–181,
196, 234
Membrane disruption ............................................ 4, 9, 13
Micronization ................................................................ 180
MicroRNA (miRNAs) ............................... 124, 125, 127,
128, 133, 135, 137–139, 184
Microscopy
confocal.............................................. 30–32, 111, 190
electron .................................................. 31, 45, 49, 54
fluorescence .........................................................29, 45
inverted ...................................................209, 215–217
MTT ...............................................................7, 12, 60, 62
Multiplicity of infection (MOI) ............................. 11, 12,
14, 24
Mutations ..................................193, 194, 196, 197, 199,
201, 204, 206, 209
242
BIO-CARRIER VECTORS: METHODS AND PROTOCOLS
Index