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(ed) Handbook of ELISPOT: methods and
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B-H, Komarovskaya M, Bersenev A, Hurwitz
ME, Krause DS, Weissman SM, Katz SG
(2019) A versatile flow-based assay for
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viability assay in a multiparametric configuration. Molecules 23(7):1575. https://doi.org/
10.3390/molecules23071575
14. Jinek M, Chylinski K, Fonfara I, Hauer M,
Doudna JA, Charpentier E (2012) A programmable dual-RNA–guided DNA endonuclease
in adaptive bacterial immunity. Science 337
(6096):816.
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ence.1225829
15. Thurtle-Schmidt DM, Lo T-W (2018) Molecular biology at the cutting edge: a review on
CRISPR/CAS9 gene editing for undergraduates. Biochem Mol Biol Educ 46(2):195–205.
https://doi.org/10.1002/bmb.21108
16. Wang H, Russa ML, Qi LS (2016) CRISPR/
Cas9 in Genome editing and beyond. Annu
Rev Biochem 85(1):227–264. https://doi.
org/10.1146/annurev-biochem-060815014607
17. Ran FA, Hsu PD, Wright J, Agarwala V, Scott
DA, Zhang F (2013) Genome engineering
using the CRISPR-Cas9 system. Nat Protoc 8
(11):2281–2308. https://doi.org/10.1038/
nprot.2013.143
18. Ceccaldi R, Rondinelli B, D’Andrea AD
(2016) Repair pathway choices and consequences at the double-Strand break. Trends
Cell Biol 26(1):52–64. https://doi.org/10.
1016/j.tcb.2015.07.009
CRISPR/Cas9-Mediated GFP Knock-in in K562 and Raji Cell Lines
229
Facchini A (2001) Calcein-acetyoxymethyl
cytotoxicity assay: standardization of a method
allowing additional analyses on recovered effector cells and supernatants. Clin Diagn Lab
Immunol 8(6):1131. https://doi.org/10.
1128/CDLI.8.6.1131-1135.2001
9. Zaritskaya L, Shurin MR, Sayers TJ, Malyguine
AM (2010) New flow cytometric assays for
monitoring cell-mediated cytotoxicity. Expert
Rev Vaccines 9(6):601–616. https://doi.org/
10.1586/erv.10.49
10. Kalyuzhny AE (2005) Chemistry and biology
of the ELISPOT assay. In: Kalyuzhny AE
(ed) Handbook of ELISPOT: methods and
protocols. Humana Press, Totowa, NJ, pp
15–31. https://doi.org/10.1385/1-59259903-6:015
11. Kandarian F, Sunga GM, Arango-Saenz D,
Rossetti M (2017) A flow cytometry-based
cytotoxicity assay for the assessment of human
NK cell activity. J Vis Exp 126:56191. https://
doi.org/10.3791/56191
12. Rabinovich PM, Zhang J, Kerr SR, Cheng
B-H, Komarovskaya M, Bersenev A, Hurwitz
ME, Krause DS, Weissman SM, Katz SG
(2019) A versatile flow-based assay for
immunocyte-mediated cytotoxicity. J Immunol
Methods 474:112668. https://doi.org/10.
1016/j.jim.2019.112668
13. Csepregi R, Temesfo ˝i V, Poo ´ r M, Faust Z, Ko ˝szegi T (2018) Green fluorescent protein-based
viability assay in a multiparametric configuration. Molecules 23(7):1575. https://doi.org/
10.3390/molecules23071575
14. Jinek M, Chylinski K, Fonfara I, Hauer M,
Doudna JA, Charpentier E (2012) A programmable dual-RNA–guided DNA endonuclease
in adaptive bacterial immunity. Science 337
(6096):816.
https://doi.org/10.1126/sci
ence.1225829
15. Thurtle-Schmidt DM, Lo T-W (2018) Molecular biology at the cutting edge: a review on
CRISPR/CAS9 gene editing for undergraduates. Biochem Mol Biol Educ 46(2):195–205.
https://doi.org/10.1002/bmb.21108
16. Wang H, Russa ML, Qi LS (2016) CRISPR/
Cas9 in Genome editing and beyond. Annu
Rev Biochem 85(1):227–264. https://doi.
org/10.1146/annurev-biochem-060815014607
17. Ran FA, Hsu PD, Wright J, Agarwala V, Scott
DA, Zhang F (2013) Genome engineering
using the CRISPR-Cas9 system. Nat Protoc 8
(11):2281–2308. https://doi.org/10.1038/
nprot.2013.143
18. Ceccaldi R, Rondinelli B, D’Andrea AD
(2016) Repair pathway choices and consequences at the double-Strand break. Trends
Cell Biol 26(1):52–64. https://doi.org/10.
1016/j.tcb.2015.07.009
CRISPR/Cas9-Mediated GFP Knock-in in K562 and Raji Cell Lines
229