78
T. Okutsu
Fig. 4.6 Results of
crystallization experiments
on a sputtered gold substrate
using ribonucleaseA.
Crystals appeared under
conditions of both gold
sputtering and light
irradiation
4.6 Future Prospective
Our recent experiments have found that crystallization is induced by a mechanism
other than photochemical reactions. The enhanced electric field induced by surface
plasmon resonance is said to have the same effect as optical tweezers. It has been
suggested that the effect of optical tweezers may trap proteins and concentrate
them locally, causing crystallization. We plan to study the phenomenon that protein
crystallization is induced by this photophysical mechanism.
References
1. McPherson, A.: Preparation and Analysis of Protein Crystals. Krieger Publishing Company,
Malabar, Florioda (1989)
2. Sugiyama, S., Maruyama, M., Sazaki, G., Hirose, M., Adachi, H., Takano, K., Murakami, S.,
Inoue, T., Mori, Y., Matsumura, H.: Growth of protein crystals in hydrogels prevents osmotic
shock. J. Am. Chem. Soc. 134, 5786–5789 (2012)
3. Sugiyama, S., Kashiwagi, K., Kakinouchi, K., Tomitori, H., Kanai, K., Murata, M., Adachi, H.,
Matsumura, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Igarashi, K.: Crystallization and
preliminary crystallographicstudies of PotA, a membrane-associated ATPase of the spermidinepreferential uptake system in Thermotoga maritima. Acta Cryst. F70, 738–741 (2014)
4. Okutsu, T.: Light-induced crystal growth. J. Jpn. Assoc. Crystal Growth 38, 160–160 (2011a)
5. Yoshikawa, H., Murai, Y., Sugiyama, S., Adachi, H., Matsumura, H., Inoue, T., Murakami, S.,
Takano, K., Mori, Y.: J. Jpn. Assoc. Crystal Growth 38, 161–168 (2011)
6. Okutsu, T.: J. Jpn. Assoc. Crystal Growth 38, 177–182 (2011b)
7. Grossweiner, L.I., Kaluskar, A.G., Baugher, J.F.: Flash photolysis of enzymes. Int. J. Radiat.
Biol. 29, 1–16 (1976)
8. Grossweiner, L.I., Usui, Y.: Sensitivity of chick embryos to ultraviolet radiation during early
embryogenesis. Photochem. Photobiol. 13,195–214 (1971)
9. Hashimoto, S.: Lysozyme dimer formation on lysozyme oxidation with Br 2 as studied by
fluorescence evolution. Int. J. Radiat. Biol. 41, 303–314 (1982)
10. Furuta, K., Horiuchi, H., Hiratsuka, H., Okutsu, T.: Photochemically induced nucleation of
ribonucleaseA enhanced by a stable protein dimer produced from the photochemical reaction
of Tyr residual groups. Crystal Growth Des. 8, 1886–1889 (2008)
T. Okutsu
Fig. 4.6 Results of
crystallization experiments
on a sputtered gold substrate
using ribonucleaseA.
Crystals appeared under
conditions of both gold
sputtering and light
irradiation
4.6 Future Prospective
Our recent experiments have found that crystallization is induced by a mechanism
other than photochemical reactions. The enhanced electric field induced by surface
plasmon resonance is said to have the same effect as optical tweezers. It has been
suggested that the effect of optical tweezers may trap proteins and concentrate
them locally, causing crystallization. We plan to study the phenomenon that protein
crystallization is induced by this photophysical mechanism.
References
1. McPherson, A.: Preparation and Analysis of Protein Crystals. Krieger Publishing Company,
Malabar, Florioda (1989)
2. Sugiyama, S., Maruyama, M., Sazaki, G., Hirose, M., Adachi, H., Takano, K., Murakami, S.,
Inoue, T., Mori, Y., Matsumura, H.: Growth of protein crystals in hydrogels prevents osmotic
shock. J. Am. Chem. Soc. 134, 5786–5789 (2012)
3. Sugiyama, S., Kashiwagi, K., Kakinouchi, K., Tomitori, H., Kanai, K., Murata, M., Adachi, H.,
Matsumura, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Igarashi, K.: Crystallization and
preliminary crystallographicstudies of PotA, a membrane-associated ATPase of the spermidinepreferential uptake system in Thermotoga maritima. Acta Cryst. F70, 738–741 (2014)
4. Okutsu, T.: Light-induced crystal growth. J. Jpn. Assoc. Crystal Growth 38, 160–160 (2011a)
5. Yoshikawa, H., Murai, Y., Sugiyama, S., Adachi, H., Matsumura, H., Inoue, T., Murakami, S.,
Takano, K., Mori, Y.: J. Jpn. Assoc. Crystal Growth 38, 161–168 (2011)
6. Okutsu, T.: J. Jpn. Assoc. Crystal Growth 38, 177–182 (2011b)
7. Grossweiner, L.I., Kaluskar, A.G., Baugher, J.F.: Flash photolysis of enzymes. Int. J. Radiat.
Biol. 29, 1–16 (1976)
8. Grossweiner, L.I., Usui, Y.: Sensitivity of chick embryos to ultraviolet radiation during early
embryogenesis. Photochem. Photobiol. 13,195–214 (1971)
9. Hashimoto, S.: Lysozyme dimer formation on lysozyme oxidation with Br 2 as studied by
fluorescence evolution. Int. J. Radiat. Biol. 41, 303–314 (1982)
10. Furuta, K., Horiuchi, H., Hiratsuka, H., Okutsu, T.: Photochemically induced nucleation of
ribonucleaseA enhanced by a stable protein dimer produced from the photochemical reaction
of Tyr residual groups. Crystal Growth Des. 8, 1886–1889 (2008)