the additional options, and we will again use ABI3, At3g24650
to search for proteins with which it interacts. You can select to
search from multiple databases that store Arabidopsis interactions by ticking the appropriate boxes below the AGI ID input
field.
3. Click “Submit.”
4. On the output page, a network graph of ABI3 interactors
appears, plus a legend, some further options, and a table of
these interactors at the bottom of the page (see Fig. 23).
5. In the network graph, the smaller nodes represent the proteins
that interact with ABI3, and the edges denote the interactions
between the proteins. Node color indicates protein subcellular
localization, partitioned according to support for a given localization. Edges colored in green indicate interactions for which
experimental evidence was obtained.
6. The default output is for the nodes to be colored according to
their subcellular localization as documented in the SUBA4
database (see above). A useful feature is to color nodes according to their expression levels in a given tissue. Clicking the
Fig. 23 Output page of an Arabidopsis Interactions Viewer 2 query with At3g24650, ABI3
70
G. Alex Mason et al.
to search for proteins with which it interacts. You can select to
search from multiple databases that store Arabidopsis interactions by ticking the appropriate boxes below the AGI ID input
field.
3. Click “Submit.”
4. On the output page, a network graph of ABI3 interactors
appears, plus a legend, some further options, and a table of
these interactors at the bottom of the page (see Fig. 23).
5. In the network graph, the smaller nodes represent the proteins
that interact with ABI3, and the edges denote the interactions
between the proteins. Node color indicates protein subcellular
localization, partitioned according to support for a given localization. Edges colored in green indicate interactions for which
experimental evidence was obtained.
6. The default output is for the nodes to be colored according to
their subcellular localization as documented in the SUBA4
database (see above). A useful feature is to color nodes according to their expression levels in a given tissue. Clicking the
Fig. 23 Output page of an Arabidopsis Interactions Viewer 2 query with At3g24650, ABI3
70
G. Alex Mason et al.