PART III MOLECULAR AND CELL BIOLOGICAL TECHNIQUES IN ARABIDOPSIS
10 Visualizing and Measuring Single Locus Dynamics
in Arabidopsis thaliana . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
Anis Meschichi and Stefanie Rosa
11 Identification and Quantification of Small RNAs. . . . . . . . . . . . . . . . . . . . . . . . . . . . 225
Di Sun, Zeyang Ma, Jiaying Zhu, and Xiuren Zhang
12 Flow Cytometry and Sorting in Arabidopsis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
David W. Galbraith and Guiling Sun
13 Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development. . . . . . 295
Monica Pia Caggiano, Xiulian Yu, Carolyn Ohno,
Pia Sappl, and Marcus G. Heisler
14 Using Genetically Encoded Fluorescent Biosensors for Quantitative
In Vivo Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 303
Akira Yoshinari, Jacob Moe-Lange, Thomas J. Kleist,
Heather N. Cartwright, David A. Quint, David W. Ehrhardt,
Wolf B. Frommer, and Masayoshi Nakamura
15 Using Tripartite Split-sfGFP for the Study of Membrane
Protein–Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 323
Tzu-Yin Liu
16 High-Pressure Freezing and Freeze Substitution for Transmission
Electron Microscopy Imaging and Immunogold-Labeling . . . . . . . . . . . . . . . . . . . 337
Marisa S. Otegui
17 Atomic Force Microscopy to Study Cell Wall Mechanics in Plants . . . . . . . . . . . . 349
Mateusz Majda
18 Identification and Characterization of Reproductive
Mutations in Arabidopsis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 371
Marı ´a Flores-Tornero, Stefanie Sprunck, and Thomas Dresselhaus
PART IV BIOCHEMICAL AND PHYSIOLOGICAL TECHNIQUES IN ARABIDOPSIS
19 Metabolomic Analysis of Natural Variation in Arabidopsis . . . . . . . . . . . . . . . . . . . 393
Si Wu, Saleh Alseekh, Yariv Brotman, and Alisdair R. Fernie
20 Untargeted Metabolomics of Arabidopsis Stomatal Immunity . . . . . . . . . . . . . . . . 413
Lisa David, Jianing Kang, and Sixue Chen
21 Biotin-Based Proximity Labeling of Protein Complexes in Planta . . . . . . . . . . . . . 425
Madiha Khan, Rajagopal Subramaniam, and Darrell Desveaux
22 Phosphoproteomic Analysis of Plant Membranes . . . . . . . . . . . . . . . . . . . . . . . . . . . 441
Lin Xi, Waltraud X. Schulze, and Xu Na Wu
23 RNA-Binding Protein Immunoprecipitation and High-Throughput
Sequencing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 453
Tino Ko ¨ster and Dorothee Staiger
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 463
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Contents
10 Visualizing and Measuring Single Locus Dynamics
in Arabidopsis thaliana . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
Anis Meschichi and Stefanie Rosa
11 Identification and Quantification of Small RNAs. . . . . . . . . . . . . . . . . . . . . . . . . . . . 225
Di Sun, Zeyang Ma, Jiaying Zhu, and Xiuren Zhang
12 Flow Cytometry and Sorting in Arabidopsis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
David W. Galbraith and Guiling Sun
13 Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development. . . . . . 295
Monica Pia Caggiano, Xiulian Yu, Carolyn Ohno,
Pia Sappl, and Marcus G. Heisler
14 Using Genetically Encoded Fluorescent Biosensors for Quantitative
In Vivo Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 303
Akira Yoshinari, Jacob Moe-Lange, Thomas J. Kleist,
Heather N. Cartwright, David A. Quint, David W. Ehrhardt,
Wolf B. Frommer, and Masayoshi Nakamura
15 Using Tripartite Split-sfGFP for the Study of Membrane
Protein–Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 323
Tzu-Yin Liu
16 High-Pressure Freezing and Freeze Substitution for Transmission
Electron Microscopy Imaging and Immunogold-Labeling . . . . . . . . . . . . . . . . . . . 337
Marisa S. Otegui
17 Atomic Force Microscopy to Study Cell Wall Mechanics in Plants . . . . . . . . . . . . 349
Mateusz Majda
18 Identification and Characterization of Reproductive
Mutations in Arabidopsis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 371
Marı ´a Flores-Tornero, Stefanie Sprunck, and Thomas Dresselhaus
PART IV BIOCHEMICAL AND PHYSIOLOGICAL TECHNIQUES IN ARABIDOPSIS
19 Metabolomic Analysis of Natural Variation in Arabidopsis . . . . . . . . . . . . . . . . . . . 393
Si Wu, Saleh Alseekh, Yariv Brotman, and Alisdair R. Fernie
20 Untargeted Metabolomics of Arabidopsis Stomatal Immunity . . . . . . . . . . . . . . . . 413
Lisa David, Jianing Kang, and Sixue Chen
21 Biotin-Based Proximity Labeling of Protein Complexes in Planta . . . . . . . . . . . . . 425
Madiha Khan, Rajagopal Subramaniam, and Darrell Desveaux
22 Phosphoproteomic Analysis of Plant Membranes . . . . . . . . . . . . . . . . . . . . . . . . . . . 441
Lin Xi, Waltraud X. Schulze, and Xu Na Wu
23 RNA-Binding Protein Immunoprecipitation and High-Throughput
Sequencing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 453
Tino Ko ¨ster and Dorothee Staiger
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 463
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Contents