R
Reproduction ............................... 99, 102, 104, 368, 371
RIP-seq .......................................................................... 454
RNA-binding protein ................................. 453, 454, 459
RNA immunoprecipitation.................................. 455, 456
RNA silencing ...................................................... 122, 225
Rootstocks ............................................................ 113–118
S
Scions .................................................................... 113–118
Seedling transformation................................................ 148
Seeds
germination ........................................ 11, 14, 18, 101,
102, 117, 149, 151, 176, 182, 207, 314, 356
quality ..................................................................11, 14
Sequence-specific nucleases (SSNs) ............................. 122
Small RNAs .................................... 35, 79, 225–252, 265
Sorting ........................................ 157, 158, 249, 255–287
Spectral analysis ..........................261, 265, 276, 279, 280
Subcellular localizations.......................................... 52, 64,
70, 74, 147, 157, 158, 326
Super-resolution imaging .................................... 158, 163
Systemic acquired resistance (SAR) .................... 416, 421
T
Transcription .............................................. 31, 43, 46, 48,
50–52, 64, 68, 70, 74, 78, 122, 213, 233, 239,
244, 265, 305, 394, 438, 453
Transcriptomics ............................................................... 46
Transient expression............................................ 148, 151,
157–164, 168, 426
Tripartite split-sfGFP ........................................... 323–334
V
Vacuum infiltration ...................... 15, 147–155, 287, 454
Vegetative meristems............................................ 295–300
ARABIDOPSIS PROTOCOLS
Index 465
Reproduction ............................... 99, 102, 104, 368, 371
RIP-seq .......................................................................... 454
RNA-binding protein ................................. 453, 454, 459
RNA immunoprecipitation.................................. 455, 456
RNA silencing ...................................................... 122, 225
Rootstocks ............................................................ 113–118
S
Scions .................................................................... 113–118
Seedling transformation................................................ 148
Seeds
germination ........................................ 11, 14, 18, 101,
102, 117, 149, 151, 176, 182, 207, 314, 356
quality ..................................................................11, 14
Sequence-specific nucleases (SSNs) ............................. 122
Small RNAs .................................... 35, 79, 225–252, 265
Sorting ........................................ 157, 158, 249, 255–287
Spectral analysis ..........................261, 265, 276, 279, 280
Subcellular localizations.......................................... 52, 64,
70, 74, 147, 157, 158, 326
Super-resolution imaging .................................... 158, 163
Systemic acquired resistance (SAR) .................... 416, 421
T
Transcription .............................................. 31, 43, 46, 48,
50–52, 64, 68, 70, 74, 78, 122, 213, 233, 239,
244, 265, 305, 394, 438, 453
Transcriptomics ............................................................... 46
Transient expression............................................ 148, 151,
157–164, 168, 426
Tripartite split-sfGFP ........................................... 323–334
V
Vacuum infiltration ...................... 15, 147–155, 287, 454
Vegetative meristems............................................ 295–300
ARABIDOPSIS PROTOCOLS
Index 465
