on protocol using the original BirA*, a similar method should be
applicable to TurboID or miniTurbo with the exception of modifying the concentration of biotin provided to the plant as well as the
labeling time (see Note 1; Table 1).
•
•
•
•
•
•
•
•
•
N
M
C
ER
G
PM
•
MO
C
Fig. 1 BioID experimental protocol (a) pDEX BASTA (pDB) BioID vector multiple cloning site sequence for
cloning in frame with an HA epitope tag (in bold green and underlined). (b) Flowchart of BioID experimental
protocol to identify (1) Global proteome of plant organelles/compartments (2) Bait-specific interactions.
Abbreviations: M mitochondria, G golgi apparatus, C chloroplast, N nucleus, ER endoplasmic reticulum, MO
membraneless organelles, PM plasma membrane
Biotin-Based Proximity Labeling of Protein Complexes in Planta
429
applicable to TurboID or miniTurbo with the exception of modifying the concentration of biotin provided to the plant as well as the
labeling time (see Note 1; Table 1).
•
•
•
•
•
•
•
•
•
N
M
C
ER
G
PM
•
MO
C
Fig. 1 BioID experimental protocol (a) pDEX BASTA (pDB) BioID vector multiple cloning site sequence for
cloning in frame with an HA epitope tag (in bold green and underlined). (b) Flowchart of BioID experimental
protocol to identify (1) Global proteome of plant organelles/compartments (2) Bait-specific interactions.
Abbreviations: M mitochondria, G golgi apparatus, C chloroplast, N nucleus, ER endoplasmic reticulum, MO
membraneless organelles, PM plasma membrane
Biotin-Based Proximity Labeling of Protein Complexes in Planta
429